This study aimed to investigate the existence of Herpes simplex virus (HSV) type1 and type 2 in abortion women as well as to detect the role of cytokines in the induction of abortion. Over the period from November (2019) to February (2020), a total of 68 cases of abortion women Collected from Al-Zahraa Teaching Hospital in AL-Najaf city, and the women’s generating of children teaching hospital Kerbala. Furthermore, the control group relied on 20 healthy pregnant women without any evidence of chronic inflammatory disease, two types of cases with ages ranging from 16-40 years. Blood samples were obtained from abortion women and healthy controls were tested for HSV-1 and HSV-2 tested first on Rapid ICT Kits. Of the 68 samples, 9(13%) were positive, and 59(87%) were negative in Rapid Immunochromatographic Test (ICT), While the results of pregnant women’s investigation showed Nil(0%) negative result for every 20 samples these findings were then verified by using Enzyme-Linked Immunosorbent Assay (ELISA) technique to investigate HSV type1 and type 2 where attained 38(56%) positive samples in the aborted women and 8(40%) positive samples in the pregnant women respectively. Collected samples from abortion women and control estimate immunological level (IL-2, IL-10 & TFN-α, C3) Used the ELISA and radial immunodiffusion method. The results revealed the sensitivity of rapid (ICT) compare to the ELISA technique was 11 %. This ratio indicates the true positive HSV infection and the specificity was 92% indicate to the true negative infection with HSV. And the positive cases of HSV-1and HSV-2 in aborted women were recorded 27(40%) at the first trimester. In another side, the other cases of abortion occurred in the second and third trimester were reached (16/68) and (1/68) respectively. The Cytokine tests. High significance (p<0.05) of IL-2 in aborted + HSV women (3.994±0.9354) and aborted -HSV women (4.655±1.423) compared to control pregnant women (2.903±0.7484) whereas there was a non-significant difference between aborted + HSV women and aborted -HSV women. The results of this study revealed a high level of IL-2 in the serum of aborted women as positive and negative for HSV compared with pregnant women. While the result of IL-10 level showed great significance (p<0.05) in aborted +HSV women(2.709 ± 0.3877),aborted -HSV women(2.905 ± 0.3368) compared with control (pregnant) women (2.267 ± 0.2332) But there is no -significant difference (p<0.05) among aborted +HSV women and aborted –HSV . On the other hand Results showed great significance (p<0.05) of TNF-α level in aborted +HSV women(4.859 ± 0.6083),aborted -HSV women(4.770 ± 0.4548) compared with control (pregnant) women (4.841 ± 0.4153) while there was non-significant difference (p<0.05) among aborted +HSV women and aborted –HSV women. In the present study, the results detected high significance (p<0.05) in the levels of C3 for aged group ≥ 42 (85.80±24.63 mg/dl)in comparison to the control(79.10 ±4.890 mg/dl).

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