In vivo effects of different doses of ciprofloxacin on glutathione reductase activity in some rat tissues
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2020 - Volume 14 Issue 2, pp. 3019-3023
  • Published Online: 17 Sep 2020
  • Open Access Full Text (PDF)


This study evaluated the effect of a fluoroquinolone antibiotic, ciprofloxacin on the enzymatic activity of glutathione reductase (GR) in the brain, cardiac, small intestine, and testes tissues of rats. GR enzymes contribute to maintaining appropriate concentrations of intracellular glutathione (GSH). Seventy-two adult male albino rats were divided into four groups (I–IV). Rats in group I (control) were injected with sterile distilled water, whereas animals in experimental groups (II-IV), were injected intraperitoneally with ciprofloxacin in single doses of 100, 150, and 200 mg/kg, respectively. Dosages effects of antibiotic were investigated at three specific periods (in the first, the third, and the seventh hour). The obtained results showed that brain GR activity in ciprofloxacin groups was inhibited compared to the control group all periods (p <0.05). After seven hours, ciprofloxacin found to significantly inhibited the enzyme activity in cardiac tissues especially in group IV (p <0.05). In the II and III groups, however, the small intestine reductase enzyme activity was not affected by ciprofloxacin doses, excluding group IV, where the enzyme activity decreased after seven hours of treatment (P <0.05). As for the GR enzyme activity in rat testis, it was a decrease compared to the control in the treated groups from the start of the third hour to the end of the period (P <0.05).


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