In vitro germplasm cold storage of fruit and berry plants of Kazakhstan


In vitro germplasm cold storage is an important part of the plant biodiversity conservation strategy, which ensures long-term reliable conservation and use of fruit and berry crops. Screening of apple, pear, cherry, raspberry, blackcurrant and strawberry plants for Cold Storage Duration on Murashige and Skoog (MS) medium without plant growth regulators (PGRs). Murashige and Skoog (MS) medium without plant growth regulators (PGRs) showed that the viability depends on the breed and genotype, better preserved berry crops, worse fruit. The effect of plant growth regulators, carbon sources and nitrate nitrogen on the storage duration in vitro of various crops in low positive temperature and illumination conditions was studied. Many genotypes were well preserved at 4°C in semi-permeable plastic tissue-culture bags for cultivation in MS medium with 3% sucrose or 2% sucrose + 2% mannitol with or without PGRs. Reducing the nitrogen concentration increased the storage duration of some crops (apple, pear), but abscisic acid did not improve the storage time. In vitro cold collection is a reserve of the field collection and now has 196 samples of fruit and berry crops stored in bags for cultivation on MS medium with Nitrate nitrogen at 50%, 0.5 mg·L-1 N6 benzylaminopurine (BAP), 0.1 mg·L-1 indole-3-butyric acid (IBA) with 3% sucrose and 0.75% agar at pH 5.7.


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