In vitro biochemical evaluation the effect of (Cobalt and Nickel-Zinc) ferrite Nanoparticles on beta -thalassemia major erythrocytes

Abstract

Objective: this study evaluate the interaction of Cobalt Ferrite (CoFe2O4) and Nickel-Zinc Iron Oxide (Ni0.5Zn0.5Fe2O4) nanoparticles with human erythrocytes in relation to the hemolytic activity and the effect of the above nanoparticles on human plasma albumin of patients with β – thalassemia major compared to healthy subjects. Design and Methods: the study include 40 β – thalassemia major patients and 20 apparently healthy subjects. The hemolysis % assay and in vitro anti-inflammatory effect of the above nanoparticles were used. Results: The results indicated that Co ferrite nanoparticles increases the fragility (the hemolysis % was higher) of the erythrocytes more readily in the case of β – thalassemia patients compared to healthy subjects and the Ni-Zn ferrite nanoparticles increase the fragility of healthy subjects erythrocytes while in the case of thalassemia patients the hemolysis % were decrease with increasing the concentration of nanoparticles. The anti-inflammatory effect of the above nanoparticles was evaluated using the inhibition of denaturation method for human plasma albumin, the results have shown significant in-vitro anti-inflammatory effect of Co ferrite nanoparticles on plasma albumin but lower than the effect of Ni-Zn ferrite nanoparticles. Conclusions: Changes in the morphological features of erythrocytes were noticed due to the interaction of the above nanoparticles. Stability of erythrocytes was observed at lower concentration of these nanoparticles and the possible mechanisms of interaction have been described.

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