Immediate cryopreservation of isolated Ghezel ram spermatogonial stem cells and subsequent co-culture with Sertoli cells preserve their survival and functional capacity
AMA 10th edition
In-text citation: (1), (2), (3), etc.
Reference: Zargarzadeh M, Tajik P, Movahedin M, Akbari G, Qasemi-Panahi B. Immediate cryopreservation of isolated Ghezel ram spermatogonial stem cells and subsequent co-culture with Sertoli cells preserve their survival and functional capacity. Eurasia J Biosci. 2019;13(2), 1129-1135.

APA 6th edition
In-text citation: (Zargarzadeh et al., 2019)
Reference: Zargarzadeh, M., Tajik, P., Movahedin, M., Akbari, G., & Qasemi-Panahi, B. (2019). Immediate cryopreservation of isolated Ghezel ram spermatogonial stem cells and subsequent co-culture with Sertoli cells preserve their survival and functional capacity. Eurasian Journal of Biosciences, 13(2), 1129-1135.

Chicago
In-text citation: (Zargarzadeh et al., 2019)
Reference: Zargarzadeh, Moein, Parviz Tajik, Mansoureh Movahedin, Ghasem Akbari, and Babak Qasemi-Panahi. "Immediate cryopreservation of isolated Ghezel ram spermatogonial stem cells and subsequent co-culture with Sertoli cells preserve their survival and functional capacity". Eurasian Journal of Biosciences 2019 13 no. 2 (2019): 1129-1135.

Harvard
In-text citation: (Zargarzadeh et al., 2019)
Reference: Zargarzadeh, M., Tajik, P., Movahedin, M., Akbari, G., and Qasemi-Panahi, B. (2019). Immediate cryopreservation of isolated Ghezel ram spermatogonial stem cells and subsequent co-culture with Sertoli cells preserve their survival and functional capacity. Eurasian Journal of Biosciences, 13(2), pp. 1129-1135.

MLA
In-text citation: (Zargarzadeh et al., 2019)
Reference: Zargarzadeh, Moein et al. "Immediate cryopreservation of isolated Ghezel ram spermatogonial stem cells and subsequent co-culture with Sertoli cells preserve their survival and functional capacity". Eurasian Journal of Biosciences, vol. 13, no. 2, 2019, pp. 1129-1135.

Vancouver
In-text citation: (1), (2), (3), etc.
Reference: Zargarzadeh M, Tajik P, Movahedin M, Akbari G, Qasemi-Panahi B. Immediate cryopreservation of isolated Ghezel ram spermatogonial stem cells and subsequent co-culture with Sertoli cells preserve their survival and functional capacity. Eurasia J Biosci. 2019;13(2):1129-35.

Abstract

Due to the limited number of spermatogonial stem cells (SSCs), an efficient preservation protocol is one of the main criteria for successful application of these cells. The aim of this study was to determine the efficiency of cryopreservation of Ghezel ram spermatogonial stem cells co-cultured with Sertoli cells. After digestion of the testicular samples from two month old lambs, obtained cells were randomly divided into 5 groups (each with 4 replicates) as follow: 1) Co-cultured with fresh Sertoli cells for 10 days (Control group). 2,3) Immediately cryopreserved (for one month) and then co-culture with/without Sertoli cells for 10 days (F0S and F0NS, respectively). 4,5) Cultured for 14 days and then cryopreserved (for one month) and finally cultured with/without Sertoli cells for 10 days (F14S and F14NS, respectively). Viability and colonization of SSCs were evaluated. The survival rate of freeze-thawed testicular cells in different treatments were not significantly different. The number of colonies in F14S, F0NS and F14NS were significantly higher than those of the control in days 5 and 10 (P < 0.05). The highest diameters of the colonies were observed in the F0S and F14S groups (P < 0.05) and the lowest diameter belonged to F0NS and F14NS treatments (P < 0.05). This study indicated that freezing the sheep SSCs can be a suitable way for their long-term maintenance. The suitable method to freeze sheep SSCs is to culture them on the day of enzymatic digestion and it can be concluded that culturing them before freezing leads to decreased ability of colonization.

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