Hydrothermal assisted biological synthesis of Silver nanoparticles by using honey and gomutra (Cow Urine) for qualitative determination of its antibacterial efficacy against Pseudomonas sp.isolated from contact lenses
AMA 10th edition
In-text citation: (1), (2), (3), etc.
Reference: Jain N, Bhosale P, Tale V, Henry R, Pawar J. Hydrothermal assisted biological synthesis of Silver nanoparticles by using honey and gomutra (Cow Urine) for qualitative determination of its antibacterial efficacy against Pseudomonas sp.isolated from contact lenses. Eurasia J Biosci. 2019;13(1), 27-33.

APA 6th edition
In-text citation: (Jain et al., 2019)
Reference: Jain, N., Bhosale, P., Tale, V., Henry, R., & Pawar, J. (2019). Hydrothermal assisted biological synthesis of Silver nanoparticles by using honey and gomutra (Cow Urine) for qualitative determination of its antibacterial efficacy against Pseudomonas sp.isolated from contact lenses. Eurasian Journal of Biosciences, 13(1), 27-33.

Chicago
In-text citation: (Jain et al., 2019)
Reference: Jain, Nishita, Preeti Bhosale, Vidya Tale, Rabinder Henry, and Jayant Pawar. "Hydrothermal assisted biological synthesis of Silver nanoparticles by using honey and gomutra (Cow Urine) for qualitative determination of its antibacterial efficacy against Pseudomonas sp.isolated from contact lenses". Eurasian Journal of Biosciences 2019 13 no. 1 (2019): 27-33.

Harvard
In-text citation: (Jain et al., 2019)
Reference: Jain, N., Bhosale, P., Tale, V., Henry, R., and Pawar, J. (2019). Hydrothermal assisted biological synthesis of Silver nanoparticles by using honey and gomutra (Cow Urine) for qualitative determination of its antibacterial efficacy against Pseudomonas sp.isolated from contact lenses. Eurasian Journal of Biosciences, 13(1), pp. 27-33.

MLA
In-text citation: (Jain et al., 2019)
Reference: Jain, Nishita et al. "Hydrothermal assisted biological synthesis of Silver nanoparticles by using honey and gomutra (Cow Urine) for qualitative determination of its antibacterial efficacy against Pseudomonas sp.isolated from contact lenses". Eurasian Journal of Biosciences, vol. 13, no. 1, 2019, pp. 27-33.

Vancouver
In-text citation: (1), (2), (3), etc.
Reference: Jain N, Bhosale P, Tale V, Henry R, Pawar J. Hydrothermal assisted biological synthesis of Silver nanoparticles by using honey and gomutra (Cow Urine) for qualitative determination of its antibacterial efficacy against Pseudomonas sp.isolated from contact lenses. Eurasia J Biosci. 2019;13(1):27-33.

Abstract

Background: Biological synthesis of Silver Nanoparticles (AgNPs) by using honey and gomutra shall become biocompatible and cost effective alternative method wherein no extra reducing agents were used which may create problems for living systems and also adds extra cost to the system.
Materials and Methods: The crude gomutra and purified honey (1 mg/mL) in sterile distilled water were used for the synthesis of Ag NPs by hydrothermal method. The characterization of Ag NPs was done by UV-Vis Spectroscopy and Scanning Tunneling Microscopy (STM) to study optical and morphological characteristics, correspondingly. The isolation of bacterial culture was carried out by incubation of swabs taken from contact lenses of healthy volunteers, followed by its identification through microscopic and biochemical studies. Antibacterial activity of Ag NPs was performed against Pseudomonas species by anti-well diffusion assay and broth dilution method. Studies were also performed for determination of biofilm reduction by Ag NPs by congo red agar test and tube assay.
Results: Silver Nanoparticles were synthesized by using honey and gomutra by hydrothermal method The absorption spectrum of Ag NPs and band gap was found to be at 350 nm and 3.54 eV respectively, for honey and 275 nm and 4.50 eV, respectively, for gomutra by UV-Vis Spectroscopy. STM image showed Ag NP’s cluster morphology on surface at 200 nm for honey and 50 nm for gomutra. Ag NPs was found to be effective antibacterial agent at the concentration of 1mg/mL against Pseudomonas predominantly present in contact lenses and therefore have a may have applications in ophthalmology.

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