Histopathology of Spodoptera Litura larva infected by Multiple Nucleopolyhedrosis Virus (SpltMNPV) in photo-protectant formulation
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2019 - Volume 13 Issue 1, pp. 185-191
  • Published Online: 05 May 2019
  • Article Views: 205 | Article Download: 92
  • Open Access Full Text (PDF)
AMA 10th edition
In-text citation: (1), (2), (3), etc.
Reference: Asri MT. Histopathology of Spodoptera Litura larva infected by Multiple Nucleopolyhedrosis Virus (SpltMNPV) in photo-protectant formulation. Eurasia J Biosci. 2019;13(1), 185-191.

APA 6th edition
In-text citation: (Asri, 2019)
Reference: Asri, M. T. (2019). Histopathology of Spodoptera Litura larva infected by Multiple Nucleopolyhedrosis Virus (SpltMNPV) in photo-protectant formulation. Eurasian Journal of Biosciences, 13(1), 185-191.

Chicago
In-text citation: (Asri, 2019)
Reference: Asri, Mahanani Tri. "Histopathology of Spodoptera Litura larva infected by Multiple Nucleopolyhedrosis Virus (SpltMNPV) in photo-protectant formulation". Eurasian Journal of Biosciences 2019 13 no. 1 (2019): 185-191.

Harvard
In-text citation: (Asri, 2019)
Reference: Asri, M. T. (2019). Histopathology of Spodoptera Litura larva infected by Multiple Nucleopolyhedrosis Virus (SpltMNPV) in photo-protectant formulation. Eurasian Journal of Biosciences, 13(1), pp. 185-191.

MLA
In-text citation: (Asri, 2019)
Reference: Asri, Mahanani Tri "Histopathology of Spodoptera Litura larva infected by Multiple Nucleopolyhedrosis Virus (SpltMNPV) in photo-protectant formulation". Eurasian Journal of Biosciences, vol. 13, no. 1, 2019, pp. 185-191.

Vancouver
In-text citation: (1), (2), (3), etc.
Reference: Asri MT. Histopathology of Spodoptera Litura larva infected by Multiple Nucleopolyhedrosis Virus (SpltMNPV) in photo-protectant formulation. Eurasia J Biosci. 2019;13(1):185-91.

Abstract

Spodoptera litura Multiple Nucleopolyhedrosis Virus (SpltMNPV) is a viral pathogen with the potential to control Spodoptera litura pests. The virus is effective in controlling S. litura, with mortality of 80–90%. Histopathologic preparations were made over the course of two ways using paraffin with hematoxylin-eosin staining and IHC methods. SpltMNPV was detected in S. litura and histopathologically characterized by the existence of polyhedral inclusion bodies of adistinctive color (dark brown), which had spread throughout the midgut lumen and epithelium. Tissues and organs were infected by SpltMNPV in vitro in a photo-protectant formula with incubation times of 0, 1, 2 and 3 days, using the paraffin method. Virus infected the lumen, midgut epithelium, epithelial skin, trachea, blood vessels, muscle cells and Malpighian tubules but not the cuticle or nerve cells. In addition, IHC revealed no SpltMNPV in muscle cells.

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