Hatching of migratory locust (Locusta migratoria, L.) (Orthoptera: acrididae) eggs at several of texture and moisture levels in semi-field laboratory


Migration locust (Locusta migratoria, L.) or Kembara (Indonesian) grasshopper is one of the herbivorous insects that lay eggs on the ground. The spawning process requires the right place, humidity, and type of soil. Moist soil and suitable soil texture can accelerate the hatching of grasshoppers into nymphs. This study aims to analyze the effect of texture and soil moisture level on hatching eggs of migratory locust (Locusta migratoria, L.). This research was carried out in a semi-field laboratory in Kefamenanu, North Central Timor Regency. The study was conducted for three months, from October to December 2018. The research method used completely randomized designs and data analysis using the Tukey's HSD (honestly significant difference) 5% test. The results showed the texture of sandy clay loam with a humidity level of 60% (24.67 days) and 80% (22.33 days) can shorten the time of hatching eggs, and at 40% humidity on the texture of the clay lasts longer for 32.33 days.


  • Clark X, Clissold FJ, Charleston MA, Simpson SJ. 2013. Foraging in a nutritionally complex world: tests using agent-based models and locusts. JOxford Univ Press Inc Dept.
  • Crooks WT, Cheke RA. 2014. Soil moisture assessments for brown locust Locustana pardalina breeding potential using synthetic aperture radar. J Applied Remote Sensing. DOI: 10.1117/1.JRS.8.084898
  • Deveson ED, Woodman JD. 2014. Embryonic diapause in the Australian plague locust relative to parental experience of comulative photophase decline. J. Insect Physiol. 70:1-7.
  • Dkhili J, Maeno K, Hassani LMI, Ghaout S, Piou C. 2019. Effects of starvation and vegetation distribution on Locust Collective Motion. Journal of Insect Behavior, Vol. 32, pp. 207–217.
  • Escorihuela MJ, Merlin O, Stefan V, Moyano G, Eweys OA, Zribi M, Kamara S, Benahi AS, Ebbe MAB, Chihrane J, Ghaout S, Cissé S, Diakité F, Lazar M, Pellarin T, Grippa M, Cressman K, Piou C. 2018. SMOS based high resolution soil moisture estimates for desert locust preventive management. Remote Sensing Applications: Society and Environment, Volume 11, pp.140-150.
  • Georgis R, Poinar GO. 2010. Effect of Soil Texture on the Distribution and Infectivity of Neoaplectana carpocapsae (Nematoda: Steinernematidae). J Nematology 15(2):308-311.
  • Gomez D, Justo JS, Salvador P, Casavova C. 2018. Machine learning approach to locate desert locust breeding areas based on ESA CCI soil moisture. J Apllied remote Sensing 12 (03):1. DOI: 10.1117/1.JRS.12.036011.
  • He J, Chen Q, Wei Y, Jiang F, Yang M, Hao S, Guo X, Chen D, Kang L. 2016. MicroRNA-276 promotes egg-hatching synchrony by up-regulating brm in locusts. DOI: 10.1073/pnas.1521098113
  • Hou L, Wang X, Yang P, Li B, Lin Z_bookmark0, Kang L, Wang X. (2020) DNA methyltransferase 3 participates in behavioral phase change in the migratory locust. Insect Biochemistry and Molecular Biology. 121.
  • Howe HF, Westley LC. 1998. Ecological Relationships of Plants and Animals. Oxford. Oxford University Press.
  • Johnson SN, McNicol JW, Gregory PJ, Oodaly Y. 2010. Effects of soil conditions and drought on egg hatching and larval survival of the clover root weevil (Sitonalepidus) J Applied Soil Ecology 44(1):75-79.
  • Jones HP. 1966. Rearing and breeding in the laboratory. Anti Locust Res. Centre. London. 12 p
  • Kalshoven LGE. 1950. De Plagen van de Cultuurgewassen in Indonesia, Dell I. Uitgev. W van Hoeve,’s Gravenhage. 512 p.
  • Kim E, Seo Y, Su Kim Y, Park Y, Ho Kim Y. 2017. Effects of Soil Textures on Infectivity of Root-Knot Nematodes on Carrot. J Plant Pathol 33(1):66-74
  • Latchininsky A, Sword G, Sergeev M, Cigliano MM, Lecoq M. 2011. Locusts and Grasshoppers: Behavior, Ecology, and Biogeography. Psyche: A Journal of Entomology, Volume 2011. DOI: 10.1155/2011/578327
  • Lazar M, Piou C, Doumandji-Mitiche B, Lecoq M. 2019. Importance of solitarious desert locust population dynamics: lessons from historical survey data in Algeria. Conference: 13th International Congress of Orthopterology, Agadir, Morocco. DOI: 10.1111/eea.12505
  • Le Gall M, Overson R, Cease A. 2019. A Global Review on Locusts (Orthoptera: Acrididae) and Their Interactions with Livestock Grazing Practices. Front. Ecol. Evol., 23 July 2019. DOI: 10.3389/fevo.2019.00263
  • Li A, Yin Y, Zhang Y, Zhang L, Zhang K, Shen J, Tan S, Shi W. 2020. Effects of Paranosema locustae (Microsporidia) on the development and morphological phase transformation of Locusta migratoria (Orthoptera: Acrididae) through modulation of the neurotransmitter taurine. Journal of Integrative Agriculture, 19 (1): 204–210. DOI: 10.1016/S2095-3119(19)62637-7
  • Lomer CJ, Johnson DL, Bateman R, Langewald J. 2001. Biological Control of Locusts and Grasshoppers. DOI: 10.1146/annurev.ento.46.1.667
  • Löw F, Walder F, Latchininsky A, Biradar C, Bolkart M, Colditz RR. 2016. Timely monitoring of Asian Migratory locust habitats in the Amudarya delta, Uzbekistan using time series of satellite remote sensing vegetation index. Journal of Environmental Management. Vol. 183, Part 3, pp. 562-575. DOI: 10.1016/j.jenvman.2016.09.001
  • Ma Z, Guo W, Guo X, Wang X, Kang L. 2011. Modulation of behavioral phase changes of the migratory locust by the catecholamine metabolic pathway. J Proc. Natl. Acad. Sci. U.S.A. 108: 3882–3887.
  • Maeno K., Tanaka S. 2012. Adult female desert locust require contact chemicals and light for progeny gregarization. J Physiol. Entomol. 37:109–118
  • Maeno KO, Ould Ely S, Nakamura S, et al. 2016. Daily microhabitat shifting of solitarious-phase Desert locust adults: implications for meaningful population monitoring. SpringerPlus 5, 107. DOI: 10.1186/s40064-016-1741-4
  • Malakhov DV, Tsychuyeva NY, Kambulin VE. 2018. Ecological Modeling of Locusta Migratoria L. Breeding Conditions in South-Eastern Kazakhstan. Russian Journal of Ecosystem Ecology, 3(1). DOI: 10.21685/2500-0578-2018-1-5
  • Matttjik AA. 2000. Perancangan Percobaan dengan Aplikasi SAS dan MINITAB. IPB Press. Bogor.
  • Mohamed Elballa TA, AbdallaYahia AM, Elkhidir EI. 2020. Studies on the Population Structure of the Tree Locust, Anacridiummelanorhodonmelanorhodon at Ennohoud Locality, West Kordofan State, Sudan. Vol. (6) 1:27-31 www.aiscience.org/journal/absj.
  • Mukai H, HironakaM,Tojo S, Nomakuchi S. 2012. Maternal vibration induces synchronous hatching in a subsocial burrower bug. J Animal Behaviour 84: 1443–1448.
  • Nishide Y, Tanaka S, Suzuki T. 2017.The hatching time of Locustamigratoria under outdoor conditions: role of temperature and adaptive significance: Locust hatching under outdoor conditions. DOI: 10.1111/phen.12184.
  • Nishide Y, Tanaka S. 2016. Desert locust, Schistocercagregaria, eggs hatch in synchrony in a mass but not when separated. J Behavioral Ecology and Sociobiology 70: 1507–1515.
  • Ping Wang Y, Ming-Fei Wu, Pei-Jiong Lin, Yao Wang, Ai-Dong Chen, Yu-Ying Jiang, Bao-Ping Zhai, Gao Hu. 2020. Plagues of Desert Locust: No invasion risk to China. doi.org/10.1101/2020.03.03.973834.
  • Qi, Xian–Lei.,Huang, Xian.,Fuxu, Hong.,& Kang, Le.,(2007). Influence of soil moisture on egg cold hardiness in the migratory locust Locustamigratoria(Orthoptera: Acridiidae) Physiological Entomology,32,219–224. DOI: 10.1111/j.1365-3032.2007.00564.x
  • Ribuan Belalang Nimfa Muncul Di Sumba Timur. Retrieved from https://www.mistar.id/nasional/ribuan-belalang-nimfa-muncul-di-sumba-timur/, June 11, 2020
  • Roonwal ML. 1936. The growth changes and structure of the egg of the African migratory locust (Locustamigratoriamigratorioides R&F (Orthoptera:Acrididae). Bull. of Entomo. Res. 27:1-14.
  • Schowalter TD. 2018. Biology and Management of the Eastern Lubber Grasshopper (Orthoptera: Acrididae). J Integrated Pest Management 9(1): 10: 1–7
  • Shmuel NB, Elena Rogovin E, Shimon Rachmilevitch S, Leonid Friedman AL, Shelef O, Hofmann I, Rosenberg T, Behar A, Shavit R, Meng F, Segoli M. 2018. Tripartite symbiosis of plant-weevil-bacteria is a widespread phenomenon in the Negev Desert. www.nature.com/scientific
  • Tanaka S, Sugahara R. 2017. Desert locusts Schistocercagregaria (Acrididae: Orthoptera) do not lay eggs in old sand: Why? DOI: 10.1007/s13355-017-0518-8.
  • Tanaka S, Toyomi K, Yudai N, et al. 2019. Effects of water extracts of frass from three locust species and various plants on oviposition and embryonic development in the desert locust, Schistocercagregaria. J Orthoptera Research 28(2): 195-204.
  • Tanaka S. 2017. Locustamigratoria (Orthoptera: Acrididae) embryos monitor neighboring eggs for hatching synchrony. J Orthoptera Research 26(2): 103-115.
  • Tokuda M, Tanaka S, Zhu D. 2010. Multiple origins of Locustamigratoria (Orthoptera: Acrididae) in the Japanese Archipelago and the presence of two major clades in the world: evidence from a molecular approach. Biological Journal of the Linnean Society, Vol. 99 (3), pp. 570–581. DOI:10.1111/j.1095-8312.2010.01386.x
  • Tu X, Hu G, Fu X, Zhang Y, Ma J, Wang Y, et al. (2019). Mass windborne migrations extend the range of the migratory locust in East China. Agricultural and Forest Entomology, DOI: 10.1111/afe.12359
  • Van Wielendaele P, Dillen S, Marchal E, et al. 2012. CRF-Like Diuretic Hormone Negatively Affects Both Feeding and Reproduction in the Desert Locust, Schistocercagregaria. PLoS ONE, 7(2): e31425. DOI: 10.1371/journal.pone.0031425
  • Woodman JD. 2015. Surviving a flood: efects of inundation period temperature and embryonic development stage in locust eggs. Bull. Entomol. Res. 105: 441-447.
  • Woodman JD. 2017. Effects of substrate salinity on oviposition, embryonic development and survival in the Australian plague locust, Chortoicetesterminifera (Walker). Journal of Insect Physiology, Vol.96, pp.9-13.
  • Yang P, Hou L, Wang X, Kang L. 2019. Core transcriptional signatures of phase change in the migratory locust. Protein & Cell. DOI: 10.1007/s13238-019-0648-6
  • Zhang L, Lecoq M, Latchininsky A, Hunter D. 2019. Locust and Grasshopper Management. Annual Review of Entomology, Vol. 64, pp.15-34. DOI: 10.1146/annurev-ento-011118-112500


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