Background: Radiographic processing involves intraoral film which contaminated with oral cavity that has Streptococcus mutans in it, then it can contaminate equipment in a dark room. Therefore, it can be the source to cross-infection. Developer solution made at Universitas Airlangga Radiology Clinic uses tap water from river in Surabaya as its solvent. The results of the tap water quality test shows various minerals such as manganese, zinc, and iron. Manganese, iron, and zinc are proven can influence the growth of Streptococcus mutans and biofilm development. This bacteria can contaminate the developer solution during the radiographic process and its growth can be affected by the mineral in the tap water as a developer solvent.
Purpose: To determine the growth of Streptococcus mutans in developer solution with water supply and aquadest as solvent and to know the differences between them.
Method: The concentrated developer was dissolved in the tap water and aquadest then routinely used at radiology clinic in dental hospital of Universitas Airlangga. Samples was taken before any films washed as control and sample of test taken 16 times each. Every sample can be taken after 8 films were washed. Control and test samples were diluted on BHIB media, planted in TYC media, and incubated for 2x24 hours. Bacteria were identified and then the number of colonies can be counted.
Result: There was growth of Streptococcus mutans as much as one colony in two samples of developer solution with a water supply solvent. There was no growth of Streptococcus mutans bacteria in the developer solution with aquadest solvent. There was no significant difference in growth of Streptococcus mutans bacteria in the solution of the developer of the water supply and the aquadest solvent.
Conclusion: The growth of Streptococcus mutans bacteria in the developer solution with a water supply solvent is more increasing than the aquadest solvent.

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