AMA 10th edition
In-text citation: (1), (2), (3), etc.
Reference: Qadir OH, Agi E, Bolhassani A, et al. Genotyping of intron 1 and 22 inversion of factor VIII gene using IS-PCR in Kurdish patients of Iraq. Eurasia J Biosci. 2020;14(1), 1181-1185.

APA 6th edition
In-text citation: (Qadir et al., 2020)
Reference: Qadir, O. H., Agi, E., Bolhassani, A., Saraji, A. A., Namvar, A., Alizadeh, S., & Shallal, A. F. (2020). Genotyping of intron 1 and 22 inversion of factor VIII gene using IS-PCR in Kurdish patients of Iraq. Eurasian Journal of Biosciences, 14(1), 1181-1185.

Chicago
In-text citation: (Qadir et al., 2020)
Reference: Qadir, Omed Hasan, Elnaz Agi, Azam Bolhassani, Alireza Azizi Saraji, Ali Namvar, Shaban Alizadeh, and Ahmed Farhan Shallal. "Genotyping of intron 1 and 22 inversion of factor VIII gene using IS-PCR in Kurdish patients of Iraq". Eurasian Journal of Biosciences 2020 14 no. 1 (2020): 1181-1185.

Harvard
In-text citation: (Qadir et al., 2020)
Reference: Qadir, O. H., Agi, E., Bolhassani, A., Saraji, A. A., Namvar, A., Alizadeh, S., and Shallal, A. F. (2020). Genotyping of intron 1 and 22 inversion of factor VIII gene using IS-PCR in Kurdish patients of Iraq. Eurasian Journal of Biosciences, 14(1), pp. 1181-1185.

MLA
In-text citation: (Qadir et al., 2020)
Reference: Qadir, Omed Hasan et al. "Genotyping of intron 1 and 22 inversion of factor VIII gene using IS-PCR in Kurdish patients of Iraq". Eurasian Journal of Biosciences, vol. 14, no. 1, 2020, pp. 1181-1185.

Vancouver
In-text citation: (1), (2), (3), etc.
Reference: Qadir OH, Agi E, Bolhassani A, Saraji AA, Namvar A, Alizadeh S, et al. Genotyping of intron 1 and 22 inversion of factor VIII gene using IS-PCR in Kurdish patients of Iraq. Eurasia J Biosci. 2020;14(1):1181-5.

Abstract

Since Hemophilia A (HA) is an endemic hereditary disease in the Middle East without a complete cure, determination of factor VIII mutations can lead to genetic diagnosis of HA in the prenatal stage. Several studies indicated that inversions of intron 22 and intron 1 (Inv22 and Inv1) in factor VIII gene are responsible for at least half of severe hemophilia A cases. In the current study, an inverse shifting-polymerase chain reaction (IS-PCR) approach was used for the molecular diagnosis of Inv22 and Inv1 in 28 Kurdish patients. Then, the coding region of the factor VIII gene was sequenced. 11 out of 28 patients had Inv22 and 2 missense, one nonsense, and deletion were found among all patients.

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