Genetic study of Thiopurin S-Methyltransferase and nudix hydrolase genes in a sample of Iraqi children
AMA 10th edition
In-text citation: (1), (2), (3), etc.
Reference: Alani MT, Al-Ani LM, Al-Halbosiy MMF. Genetic study of Thiopurin S-Methyltransferase and nudix hydrolase genes in a sample of Iraqi children. Eurasia J Biosci. 2020;14(1), 1065-1070.

APA 6th edition
In-text citation: (Alani et al., 2020)
Reference: Alani, M. T., Al-Ani, L. M., & Al-Halbosiy, M. M. F. (2020). Genetic study of Thiopurin S-Methyltransferase and nudix hydrolase genes in a sample of Iraqi children. Eurasian Journal of Biosciences, 14(1), 1065-1070.

Chicago
In-text citation: (Alani et al., 2020)
Reference: Alani, Meena Thaaer, Louay M. Al-Ani, and Mohammed M. F. Al-Halbosiy. "Genetic study of Thiopurin S-Methyltransferase and nudix hydrolase genes in a sample of Iraqi children". Eurasian Journal of Biosciences 2020 14 no. 1 (2020): 1065-1070.

Harvard
In-text citation: (Alani et al., 2020)
Reference: Alani, M. T., Al-Ani, L. M., and Al-Halbosiy, M. M. F. (2020). Genetic study of Thiopurin S-Methyltransferase and nudix hydrolase genes in a sample of Iraqi children. Eurasian Journal of Biosciences, 14(1), pp. 1065-1070.

MLA
In-text citation: (Alani et al., 2020)
Reference: Alani, Meena Thaaer et al. "Genetic study of Thiopurin S-Methyltransferase and nudix hydrolase genes in a sample of Iraqi children". Eurasian Journal of Biosciences, vol. 14, no. 1, 2020, pp. 1065-1070.

Vancouver
In-text citation: (1), (2), (3), etc.
Reference: Alani MT, Al-Ani LM, Al-Halbosiy MMF. Genetic study of Thiopurin S-Methyltransferase and nudix hydrolase genes in a sample of Iraqi children. Eurasia J Biosci. 2020;14(1):1065-70.

Abstract

An essential anti-cancer drugs in Acute Lymphoblastic Leukemia treatment protocols since long time are Thiopurines. In addition, thiopurines are also used in the treatment of chronic inflammatory bowel diseases, autoimmune diseases and as immunosuppressant after organ transplantation. However, 6-Mercaptopurine can cause unexpected frequent toxicities in Acute Lymphoblastic Leukemia patients because of low activity of thiopurine S-methyltransferase. Thiopurine, intolerance is related to genetic polymorphism in Nudix hydrolase 15 and thiopurine S-methyltransferase. To measure the frequency of thiopurine S-methyltransferase and Nudix hydrolase 15 polymorphisms and assess whether they are predictors of 6-Mercaptopurine intolerance in Iraqi children treated for Acute Lymphoblastic Leukemia. Results showed that mutant paterns AG present in patients and controls, with genotypic frequency of (18%) in patients and (12%) in controls. Whereas, the homozygous mutant type (GG) for thiopurine S methyltransferase gene and homozygous and heterozygous mutant type (CT/TT) for Nudix hydrolase Nudix hydrolase 15 did not detected in patients and controls. Heterozygous mutant type (AG) of SNP 719A>G was significant in 6-Mercaptopurine resistant to Acute Lymphoblastic Leukemia patients as compared with patients don’t have resistant to 6-Mercaptopurine and showed good response in Acute Lymphoblastic Leukemia patients with P≤0.05. These explorative results lead to propose the prospect of exploration the SNPs 719A>G as biomarkers to predict the expected respons to 6-Mercaptopurine in Acute Lymphoblastic Leukemia patients before using 6-Mercaptopurine as a treatment.

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