Present study aimed to characterization Pishdar shepherd dogs which is a native breed specified in Kurdistan- Iraq. For this purpose the RAPD markers were used to study genetic diversity among nine geographical locations in Suliamani governorate. A total of 74 samples were typed using twenty RAPD primers. Moreover, fourteen out of the twenty primers had clear bands. A total of 709 bands were scored, of which 57 bands (51.48%) were polymorphic and 15 of polymorphic band were unique bands. For all regions, Nei’s gene diversity, Shannon index, percentage of polymorphic loci and unique bands are in the range of 0.19 to 0.49, 0.34 to 0.68, 10 to 100, and 1 to 5, respectively. The UPGMA dendrograms showed three clusters, the 1st cluster branch consisted of the Sitak and Halabja, the 2nd cluster was include both of the Qala-Diza and Rania and the 3rd one included constitutes four sub-clumps the 1st branch consist of (Dokan and Suliamani) region, the 2nd branch harbored the Huwana region only. The 3rd one covers the Sangasar region. Finally, the 4th sub-cluster possesses the Pishdar group. The results indicated that impressive logical result, showed low genetic distance between the Dokan and Suliamani population, in addition to small genetic distance between Qala-Diza and Rania, and moderate genetic distance between Sitak and Halabja. Which means there was no genetic variation in between these populations according to the near geographical distance between these areas. Thus, the inbreeding mating among these areas records high value. Meanwhile, the Huwana, Sangasar and Pishdar sub-clusters population documented a moderate genetic distance between them. Nevertheless, the high genetic distance that recorded (56.13%) among the region’s population of Pishdar dog showed ample ground for mating within this breed in suliamani province.


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