Genetic detection of in vA, sipB, SopB and sseC genes in Salmonella spp isolated from diarrheic children patients
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2020 - Volume 14 Issue 2, pp. 3085-3091
  • Published Online: 17 Sep 2020
  • Open Access Full Text (PDF)

Abstract

Salmonella Gram-negative bacteria infect human and animals and cause gastroenteritis and typhoid fever. Type III secretion system are important virulence factor of salmonella it basically and functionally associated with flagella meeting systems and typically comprise more than 20 proteins subunits that are found in the internal and external membrane of the bacterial cell. The current study aims to conduct an detection the candidates genes of type III secretion system as important virulence factor of a clinical Salmonella spp. The Patient and methods: current study includes 120 stool samples collected from 120 diarrheic children, age range from (2 to 3 years) for the period from November (2018) to December (2018) from Babylon province Iraq. After culturing samples, Salmonella spp.diagnosed. DNA extraction and PCR were achieved for detection type III secretion system genes of Salmonella spp. The results: out of 120 stool sample, 58 samples were positive for Salmonella spp. we revealed that Salmonella typhi, Salmonella typhimurium, Salmonella arizonae, and Salmonella paratyphi, the most common serovar of Salmonella enterica, was Salmonella typhi at 29.3%. Genetic detection of type III secretion system by PCR technique explains that Inva A was found about at all isolates except one isolate at percentage (93.1%) and SipB occurrence was (18.9%) finally SseC occurrence was (1.7%) whereas SopB was not detected in all salmonella isolates. inva A gene is most reliable gene in the diagnosis of Salmonella spp. and not all isolates contain a different genes of type III secretion system.

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