Background: Diabetes Mellitus (DM) is the most prevalent metabolic unrest affecting the people all over the world. Vitamin B12 (Vit B12), is a water-soluble vitamin, one of the eight B vitamins, metabolically important Vit B12 deficiency later will consequence in diabetes-related complications. current data was undertaken to exploration of the relationship of Vit B12 with some anthropometric measurements in type2 diabetic patients. Materials and methods: This study consisted of 60 patients with T2DM, attending Al- Ramadi teaching hospital and Al-Fallujah teaching hospital and 24 healthy individuals as controls. Serum Vit. B12 level was determined by ELISA technique while weight, height, waist, thoracic, neck, and hip circumferences carefully measured, also body mass index (BMI), waist/neck ratio (W/N), waist/thoracic ratio (W/T), waist/hip ratio (W/H) and were documented. Results: Vitamin B12, was found to be decreased in the patients with T2DM as compared to controls (P< 0.000l), and waist to hip ratio, waist to thoracic, and Body mass index were importantly greater in T2DM patients than in HCs with (P < 0.000l) for these parameters. Vit. B12 has important negative correlation with fasting serum glucose (FSG), weight, (P<0.001), HC, NC, BMI (p<0.05), W/H, W/T (p>0.05), while positive relationship between thoracic circumference (TC) and W/N (p= 0.05) was noticed. Studied variables presented the following descending order of area under the receiver operating characteristic (AUROC) FSG (1), BMI (0.875 (Vit. B12 (0.825), W/H (0.8743), W/T (0.7837), weight (0.764), W/N (0.6715), W. C (0.7646) H. C (0.6444). Conclusion: Serum Vit. B12 level can be used as a novel biomarker in identification type 2 DM, also BMI, W/H and W/T may be a good biomarker in diagnosis type 2 DM.

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