Condylomata acuminata also known as genital wart result from the infection with human papilloma viruses particularly HPV type6 &11.Thirty three samples (blood and skin tissue) were accumulated from patient with genital warts (females) whom visit dermatology department of Hilla hospital/Iraq, with thirty three sera gathered from healthy persons act as control group. The diagnosis was achieved via dermatologist and by applied in situ hybridization (ISH) test to detect HPV types 6&11 .The estimation of IgE and TNF α levels at patient sera were done by using ELISA test. The result show that About 92% of patient with genital wart donated appositive result to the infection with HPV type 11 and 6,and there were significant increasing in IgE levels at patients sera compared with control group Mean ± S.E[202.36 ±10.16, 24.12± 1.68 IU/ML]and also TNF α show increasing in their level at patients sera compared with control groups Mean ± S.E[68.18 ± 1.72, 46.68 ± 1.13 pg/ml].

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