Evaluation of cellular oxidative stress levels in aedes aegypti mosquitoes as a reaction of photo catalyst modify nanoparticles exposure


Objectives to estimate the cellular malondialdehyde (MDA) level– as a measure of lipid peroxidation in Aedes aegypti with exposure by Nanoparticles Photo Catalysts, Mg-doped TiO2, this study also aimed to evaluate the difference in MDA levels in each of life stage and between treatment group and control. Treatment and a case-control study including 100 adults (50 females and 50 male) 100 larva and 100 pupae Aedes aegypti whose follow-up was carried out at the Virology Lab. All the subjects were subjected to estimation malondialdehyde (MDA) at the time of admission, as a marker of lipid peroxidation, and hence an indicator of free radical activity by Nanoparticles Photo Catalysts, Mg-doped TiO2, the Nano powder prepared by modification of the TiO2 band gap by doping with Mg ،Mg) atoms using sol-gel method. XRD and AFM recognition shows clear peaks assigned to TiO2 (mainly peaks: 2theta = 45 and 52o) indicating that a Mg, ions were safely incorporated into the titanium anatase framework. All sample treated with manpower than This method is based on the principle that acetic acid detaches the lipid and protein of a tissue, thiobarbituric acid reacting with lipid peroxide, hydroperoxide, and oxygen-labile double bond to form the color adducts with maximal absorbance at 530 nm. Student ‘t’ test was used for unpaired samples to compare the means of the control and the cases and also the various inter-group differences. Significance was accepted if the null hypothesis was rejected at p < 0.05. The difference in MDA levels in cases and controls was seen to be statistically significant (p < 0.001), suggesting an increase in the level of lipid peroxides The mean MDA level in the control population was 1.9 ± 0.4 nmol/dl (1.9 ± 0.1 in males; 1.9 ± 0.8 in females), while the corresponding value in pupae and larva groups were 4.16 ± 1.04 nmol/dl (4.01 ± 1.9 in pupae; 4.5 ± 1.1 in larva) and 4.03 ± 1.1 nmol/dl (3.76 ± 0.7 in males. In conclusion, in present found high concentrations of products derived from lipid peroxidation while assessing levels of an adult, larvae, pupae, and the oxidative damage of circulating protein according to the carbonyl content of cellular protein peroxidation. Our results suggest the association of cellular damage caused by oxygen free radicals with the pathogenesis of antioxidant system in Aedes aegypti of presented highly marked modifications related to the presence of nanoparticles oxidative stress, characterized by intense lipid and protein peroxidation and reduced antioxidant defense system of Aedes aegypti.


  • Ahmad S. (1995), ‘Oxidative Stress From Environmental Pollutants’, Archives of Insect Biochemistry and Physiology, 29(2): 135-157.
  • Alexander Aguirre-Obando O. & Julia Pietrobon A. & Caroline Dalla Bona A. & Mário Antônio Navarro S. (2019), Contrasting patterns of insecticide resistance and knockdown resistance (kdr) in Aedes aegypti populations from Jacarezinho (Brazil) after a Dengue Outbreak. open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).http://dx.doi.org/10.1016/j.rbe.2015.11.009.
  • Al-Seeni, M. N., M Ali, A., Elsawi, N. M., & Abdo, A. S. (2017). Assessment of the Secondary Metabolite Patulin and Lycium Barbarum Fruit on INS-1 Rat Pancreatic?-Cells. Agriculture and Food Sciences Research, 4(1), 24-29
  • Ananpattarachai J. & Kajitvichyanukul P. & Seraphin S. (2009), Visible light absorption ability and photocatalytic oxidation activity of various interstitial N-doped TiO2 prepared from different nitrogen dopants. J. Hazard. Mater, 168, 253-261.
  • Asahi R. & Morikawa T. & Irie H. & Ohwaki T. (2014), Nitrogen-Doped Titanium Dioxide as Visible-Light-Sensitive Photocatalyst: Designs,Developments, and Prospects. Chem. Rev., 114, 9824-9852.
  • Barkul R. & Koli V. & Shewale V. & Patil M. & Delekar S. (2016), Visible active nanocry stalline N-dopedanatase TiO2 particles for photocatalytic mineralization studies. Mater. Chem. Phys., 173, 42–51.
  • Beauchamp C. & Fridovich I. (1971), ‘Superoxide Dismutase: Improved Assays and an Assay Applicable to Acrylamide Gels’, Analytical Biochemistry, 44, 276-287.
  • Bradford M. (1976), ‘A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-dye Binding’, Analytical Biochemistry, 72, 248-254.
  • Caratto V. & Setti L. & Campodonico S. & Carnasciali M. M. & Botter R. & Ferretti M. (2012), Synthesis and characterization of nitrogen-doped TiO2 nanoparticles prepared by sol–gel method. J Sol-Gel Sci Technol DOI 10.1007/s10971-012-2756-0.
  • Chance B. & Machly A. (1955), ‘Assay of Catalases and Peroxidases’, in Methods in Enzymology, eds. S.P. Colowick and N.O. Kaplan, New York: Academic Press, pp. 764-775.
  • Cherng Shii L. & Indra Vythilingam M. & Li Wong Wan Y. & Wan Sulaiman Y. & Ling L. (2019), Aedes aegypti (Linnaeus) larvae from dengue outbreak areas in Selangor showing resistance to pyrethroids but susceptible to organophosphates. Acta Tropica 185 115–126. https://doi.org/10.1016/j.actatropica.2018.05.008.
  • Felton G. & Summers C. (1995), ‘Antioxidant Systems in Insects’, Archives of Insect Biochemistry and Physiology, 29(2): 187-197.
  • Gomes J. & Leal I. & Bednarczyk K. & Gmurek M. & Stelmachowski M. & Zaleska-Medynska A. & Bastos F. & Quinta-Ferreira M. & Costa R. & Quinta-Ferreira R. (2017), Detoxification of Parabens Using UV-A enhanced by Noble Metals-TiO2 Supported Catalysts. J. Environ. Chem. Eng, 5, 3065–3074.
  • Gomes J. & Lopes A. & Gmurek M. (2019), Quinta-Ferreira, R.M.; Martins, R.C. Study of the influence of the matrix characteristics over the photocatalytic ozonation of parabens using Ag-TiO2. Sci. Total Enviorn, 646, 1468–1477. .
  • Han-Dong W. & Qi-Zhi L. (2012), Antioxidative responses in Galleria mellonella larvae infected with the entomopathogenic nematode Heterorhabditis sp. Beicherriana. Biocontrol Science and Technology, Vol. 22, No. 5, 601-606.
  • Henderson M. (2011), A surface science perspective on TiO2 photocatalysis. Surf. Sci. Rep., 66, 185–297.
  • Irie H. & Watanabe Y. & Hashimoto K. (2003), Nitrogen concentration dependence onphotocatalytic activity fTiO2-x Nx Powders. J. Phys. Chem. B, 107, 5483–5486.
  • Jain S. & Levine S. (1995), ‘Elevated Lipid Peroxidation and Vitamin E-quinone Levels in Heart Ventricles of Streptozotocin-treated Diabetic Rats’, Free Radical Biology and Medicine, 18, 337341.
  • Jirakanjanakit N. & Saengtharatip S. & Rongnoparut P. & Duchon S. & Bellec C. Yoksan S. (2007), Trend of Temephos resistance in Aedes (Stegomyia) mosquitoes in Thailand during 2003–2005. Environ. Entomol. 36, 506–511.
  • João G. & João L. & Eva D. & Rosa M. & Quinta F. & Rui C. (2019), (N–TiO2 Photocatalysts: A Review of Their Characteristics and Capacity for Emerging Contaminants Removal. www.mdpi.com/journal/water. Water 11, 373; doi: 10.3390/w11020373
  • Macoris M. & Andrighetti M. & Garbelot V. & De Carvalho L. & Júnior A. & Brogdon W. (2007), Association of insecticide use and alteration on Aedes aegypti susceptibility status. Mem. Inst. Oswaldo Cruz, 102(8): 895–900.
  • Macoris M. & Camargo M. & Silva I. & Takaku L. & Andrighetti M. (1995), Modificac¸ ão da susceptibilidade de Aedes (Stegomyia) aegypti ao temephos. Rev. Patol. Trop. 24, 31-40.
  • Maxwell P. & Chen G. & Webster J. & Dunphy G. (1994), ‘Stability and Activities of Antibiotics Produced During Infection of the Insect Galleria mellonella by Two Isolates of Xenorhabdus nematophilus’, Applied Environmental Microbiology, 60, 715-721.
  • Mracek Z. & Webster J. (1993), ‘Survey of Heterorhabditidae and Steinernematidae (Rhabditida: Nematoda) in Western Canada’, Journal of Nematology, 25, 710-717.
  • Nolan N. & Synnott D. & Seery M. & Hinder S. & Wassenhoven A. & Pillai S. (2012), Effect of N-doping on the photocatalytic activity of sol–gel TiO2. J. Hazard. Mater, 211–212, 88–94.
  • Rahman I. & Macnee W. (2000), ‘Regulation of Redox Glutathione Levels and Gene Transcription in Lung Inflammation: Therapeutic Approaches’, Free Radical Biology and Medicine, 28(9): 1405-1420.
  • Ramandi S. & Entezari M. & Ghows N. (2017), Sono-synthesis of solar light responsive S-N-C-tri doped TiO2 photo-catalyst under optimized conditions for degradation and mineralization of Diclofenac. Ultrason. Sonochem, 38, 234–245.
  • Selvaraj A. & Sivakumar S. & Ramasamy A. & Balasubramanian V. (2013), Photocatalytic degradation of triazine dyes over N-doped TiO2 in solar radiation. Res. Chem. Intermed., 39, 2287–2302.
  • Simon L. & Fatrai Z. & Jonas D. & Matkovics B. (1974), ‘Study of Peroxide Metabolism Enzymes During the Development of Phaseolus vulgris’, Biochemie und Physiologie der Pflanzen, 166, 387-392.
  • Toubarro D. & Lucena-Robles M. & Nascimento G. & Costa G. & Montiel R. & Coelho A.& Simo ˜es N. (2009), ‘An Apoptosis-inducing Serine Protease Secreted by the Entomopathogenic Nematode Steinernema carpocapsae’, International Journal for Parasitology, 39(12): 1319-1330.
  • Valentin C. & Pacchioni G. & Selloni A. (2004), Origin of the different photoactivity ofN-doped anatase and rutile TiO2. Phys. Rev. B Condens. Matter Mater. Phys., 70, 1–4.
  • Wang P. & Huang B. & Dai Y. & Whangbo M. (2012), Plasmonic photocatalysts: Harvesting visible light with noble metal nanoparticles, Phys Chem. Chem. Phys., 14, 9813-9825.
  • Wang Y. & Oberley L.& Murhammer D. (2001), ‘Evidence of Oxidative Stress Following the Viral Infection of Two Lepidopteran Insect Cell Lines’, Free Radical Biology and Medicine, 31(11): 1448-1455.
  • Zhang J. & Ahmad S. & Wang Y. & Han Q. & Zhang J. & Luo Y. (2019), Cell death induced by α-terthienyl via reactive oxygen species-mediated mitochondrial dysfunction and oxidative stress in the midgut of Aedes aegypti larvae, Free Radical Biology and Medicine, doi: https:// doi.org/10.1016/j.freeradbiomed.2019.04.021.
  • Zheng Z. & Huang B. & Qin X. & Zhang X. & Dai Y. & Whangbo M. (2011), Facile in situ synthesis of visible-light plasmonic photocatalysts M@TiO2 (M = Au, Pt, Ag) and evaluation of their photocatalytic oxidation of benzene to phenol. J. Mater. Chem., 21, 9079-9087.


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