Evaluation of cellular oxidative stress levels in aedes aegypti mosquitoes as a reaction of photo catalyst modify nanoparticles exposure

Abstract

Objectives to estimate the cellular malondialdehyde (MDA) level– as a measure of lipid peroxidation in Aedes aegypti with exposure by Nanoparticles Photo Catalysts, Mg-doped TiO2, this study also aimed to evaluate the difference in MDA levels in each of life stage and between treatment group and control. Treatment and a case-control study including 100 adults (50 females and 50 male) 100 larva and 100 pupae Aedes aegypti whose follow-up was carried out at the Virology Lab. All the subjects were subjected to estimation malondialdehyde (MDA) at the time of admission, as a marker of lipid peroxidation, and hence an indicator of free radical activity by Nanoparticles Photo Catalysts, Mg-doped TiO2, the Nano powder prepared by modification of the TiO2 band gap by doping with Mg ،Mg) atoms using sol-gel method. XRD and AFM recognition shows clear peaks assigned to TiO2 (mainly peaks: 2theta = 45 and 52o) indicating that a Mg, ions were safely incorporated into the titanium anatase framework. All sample treated with manpower than This method is based on the principle that acetic acid detaches the lipid and protein of a tissue, thiobarbituric acid reacting with lipid peroxide, hydroperoxide, and oxygen-labile double bond to form the color adducts with maximal absorbance at 530 nm. Student ‘t’ test was used for unpaired samples to compare the means of the control and the cases and also the various inter-group differences. Significance was accepted if the null hypothesis was rejected at p < 0.05. The difference in MDA levels in cases and controls was seen to be statistically significant (p < 0.001), suggesting an increase in the level of lipid peroxides The mean MDA level in the control population was 1.9 ± 0.4 nmol/dl (1.9 ± 0.1 in males; 1.9 ± 0.8 in females), while the corresponding value in pupae and larva groups were 4.16 ± 1.04 nmol/dl (4.01 ± 1.9 in pupae; 4.5 ± 1.1 in larva) and 4.03 ± 1.1 nmol/dl (3.76 ± 0.7 in males. In conclusion, in present found high concentrations of products derived from lipid peroxidation while assessing levels of an adult, larvae, pupae, and the oxidative damage of circulating protein according to the carbonyl content of cellular protein peroxidation. Our results suggest the association of cellular damage caused by oxygen free radicals with the pathogenesis of antioxidant system in Aedes aegypti of presented highly marked modifications related to the presence of nanoparticles oxidative stress, characterized by intense lipid and protein peroxidation and reduced antioxidant defense system of Aedes aegypti.

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