Evaluating the effect of different doses of gamma radiation on carbohydrates, proteins, and lipids content of arthrospira fusiformis
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2020 - Volume 14 Issue 1, pp. 869-876
  • Published Online: 15 Apr 2020
  • Open Access Full Text (PDF)

Abstract

Microalgae, particularly Arthrospira sp., has been considered as an alternative energy for biofuel worldwide. It is one of the world’s largest organic nutrient sources and a potential source of biodiesel. Genetic analysis is an essential process of morphological identification confirming cyanobacterium homologous to the isolates identified in 100% of Arthrospira fusiformis at the National Center for Biotechnology Information (NCBI) GenBank. The 16S rRNA sequences obtained by the genetic analyzer were deposited in the NCBI GenBank with accession number: MN160402.1. Arthrospira fusiformis was exposed to different doses of gamma irradiation 1, 2, 4, 6, 8, 10, and 12 Gray (Gy) using Co60 as a gamma source. While the highest value of chlorophyll-a and carotenoid registered in the control unit was 7.437 and 6.733 µg/L, the lowest value was 0.423 and 0.643 µg/L at radiation treatment with 12gy, respectively. Likewise, carbohydrates reached the highest value (1.383 mg/L) in the control unit and the lowest (0.713 mg/l) in the radiation treatment with 12 Gy. Regarding proteins, the highest value was 640,387 mg/L when treated with 1 Gy, and the lowest value was 64,187 mg/L in the control unit. The highest value of total lipids was 0.633 g/L in the radiation treatment with 12 Gy, whereas the lowest value in the control unit was 0.283 g/L. These results confirmed the role of gamma irradiation in stimulation of proteins and total lipid content in Arthrospira fusiformis, which indicate the importance of gamma radiation in biodiesel production by algae species.

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