Evaluating bioactivity of benzo[a]pyrene toward expression of cd4+cd62l+, cd8+cd62l+, and cd4+ifnÿ in the biology of measles paramyxovirus-vaccinated mice


Current prevention strategies suggest that immunization is still the best option against measles and can manipulate the biologic characters of these viruses. However, immunization is often ineffective in a number of children because of the biological trait of different ages. To a significant extent, this phenomenon is caused by internal and external factors that influence children’s immunity. One of the external factors in this regard is the benzo[a]pyrene pollutant, both directly and indirectly. Accumulated benzo[a]pyrene can infiltrate deoxyribonucleic acid (DNA) to form benzo[a]pyrene and AhR bonds (Aryl Hydrocarbon Receptor). Such bonds affect the differentiation of immune cells, resulting in immunosuppressive activity. This study aims to analyze the bioactivity of benzo[a]pyrene with respect to the expression of CD4+CD62L+, CD8+CD62L+, and CD4+IFNŸ in a measles paramyxovirus-vaccinated mouse. Approximately 20 mg/kg BW of benzo[a]pyrene was injected using an intramuscular approach twice per week for one month. Furthermore, flow cytometric analysis was performed to determine immune cell expression. The results showed that mice under-vaccination and benzo[a]pyrene treatment suppress the expression of CD4+CD62L+ naïve T cells, increases the expression of CD8+CD62L+ naïve T cells, and reduces the expression of CD4+IFNŸ. Controlling internal and external factors enhanced the effectiveness of measles immunization in children.


  • Akpan EA, Udoh VS (2017) Evaluation of Cassava (Manihot Esculenta crantz) Genotype for Yield and Yield Component, Tuber Bulking, Early Maturity in Cross River Basin Flood Plains, Itu, Akwa Ibom State, Nigeria. Canadian Journal of Agriculture and Crops 2(2): 68-73.
  • Al–Taai EMF (2016) Protective Effects of Sweet Orange Peel (Citrus Sinensis L.) The Induction of Micronuclei Induced by Cyclophosphamide in Human Peripheral Lymphocytes. Journal of Food Technology Research 3(1): 28-35.
  • Baratawidjaya KG (2010) Imunologi Dasar, Edisi Ketiga, Penerbit Fakultas Kedokteran Universitas Indonesia, Jakarta.
  • Brown WH, Foote CS, Iverson BL, Anslyn EV (2009) Organic Chemestry. USA, Brooks/Cole Cengange Learning.
  • Christopher L Karp (2006) Imunological Reviews 168(1): 91-101. Retrieved from https://e-resources,perpusnas,go.id:2619/doi/10.1111/j.1600-065X.1999.tb01282.X
  • Davila DR (1996) Human T Cells Are Heigly Sensitive To Supression of Mitogenesis By Polycyclic Aromatic Hidrocarbons and This Effect Differentially Reserved By Alpha-Naphthoflavone, Toxicol Appt,Pharmacol.
  • Errayes AO, Abdussalam-Mohammed W, Darwish MO (2020) Review of Phytochemical and Medical Applications of Annona Muricata Fruits. Journal of Chemical Reviews 2(1): 70-79.
  • Esser C (2009) The Immune Phenotype of AhR Null Mouse Mutants : Not a Simple Miror of Xenobiotik Receptor Over-Activation. Biochem Pharmacol 77: 597-607.
  • Fanali LZ, Franco-Belussi L, Bonini-Domingos CR, de Oliveira C (2018) Effects of benzo [a] pyrene on the blood and liver of Physalaemus cuvieri and Leptodactylus fuscus (Anura: Leptodactylidae). Environmental pollution. Jun 1;237: 93-102.
  • Hengartner H (1996) Decreased Tumor Surveilance in Perforin Deficient Mice. J. Exp. Med 184(1): 1781-1790.
  • Holladay SD, Smith BJ (1994) Fetal Hematopoietic Alterations After Maternal Exposure to Benzo[a]pyrene: a cytometric evaluation. J. Toxicol Environ Health 42: 259-73.
  • Izah, S. C. (2019). Activities of crude, acetone and ethanolic extracts of Capsicum frutescens var. minima fruit against larva of Anopheles gambiae. J Environ Treat Tech, 7, 196-200.
  • Kendrad Nelson (2015) John Hopkins; WHO; Unaids.
  • Kumar A, Upaddhyay G, Modi DR, Sing MP (2007) The Involvementnof Secondary Signaling Molecules in Cytochrome P-450 1A1- Mediated Inducible Nitric Oxide Synthase Expression in Benzo[a]pyrene-Treated Rat Polymorphonuclear Life Sci, 2007, Nuv 30:81 (23-24): 1575-1584. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/177991490?dopt=abstract
  • Laupeze B, Amiot L, Sparfel L, Ferrec EL, Fauchet R, Fardel O (2002) Polycyclic aromatic hydrocarbons effect functional differentiation and maturation of human monocyte derived dendritic cells. J. Imunol. 168:2652-2658
  • Platonova RI, Pankaj V, Olesova MM, Osipova VV, Platonova AZ, Evseeva MM (2019) Modernization of Secondary Vocational Education System. Journal of Environmental Treatment Techniques 7(4): 562-565.
  • Rifa’i M (2013) Imunologi & Alergi Hiper Sensitif, UB Press, Malang.
  • Rifa’i M (2018) Autoimun & Bioregulator Edisi Revisi, UB Press, Malang.
  • Rudolph A (2006) Buku ajar pediatri, Jakarta, EGC
  • Sarnia S, Ibrahim MN, Isamu KT (2018) Karakteristik Ikan Gabus (Channa Striata) Asap Dari Produsen Yang Berbeda Di Kabupaten Konawe Sulawesi Tenggara. Jurnal Fish Protech 1(1).
  • Sysa A, Labai M, Kvasyuk E, Ivuts U, Khanchevskii M (2019) Influence of arabinofuranosylcytosine-5-monophosphate and its emoxipin salt on viability and functional status of peripheral blood lymphocytes subpopulations. Progress in Chemical and Biochemical Research: 178-184.
  • Untung Suseno (2014) Profil Kesehatan Indonesia, Kementerian Kesehatan Republik Indonesia.


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