Eurasian Journal of Biosciences


Aim: the aim of the present systematic review and meta-analysis was evaluate the levels changes of periodontal pathogens in children during orthodontic treatments.
Method: From the electronic databases, PubMed, Cochrane Library, Embase, ISI have been used to perform a systematic literature until September 2020. Therefore, a software program (Endnote X8) has been utilized for managing the electronic titles. Searches were performed with mesh terms. Odds ratio between before and one or three months after treatment with 95% confidence interval (CI), Mantel-Haenszel method were calculated. Random effects were used to deal with potential heterogeneity and I2 showed heterogeneity. The Meta analysis and forest plots have been evaluated with the use of a software program available in the market (i.e., Comprehensive Meta-Analysis Stata V16).
Result: A total of 46 potentially relevant titles and abstracts were found during the electronic and manual search. Finally, a total of three publications fulfilled the inclusion criteria required for this systematic review. Odds ratio of P.gingivalis was (OR, -0.58 95% CI -1.34, 0.18. P= 0.14) and
Odds ratio of T. forsythensis was (OR, -1.44 95% CI -2.16, 0.73. P= 0.00), also odds ratio of P. intermedia was (OR, -1.09 95% CI -2.09, -0.10. P= 0.03).
Conclusion: The present systematic review and met analysis showed the levels of periodontal pathogens in children during orthodontic treatments was increased after orthodontic treatments vs before orthodontic treatments. Only no change in P.gingivalis was observed.


  • Abed Al Jawad, F., Cunningham, S. J., Croft, N., & Johal, A. (2012). A qualitative study of the early effects of fixed orthodontic treatment on dietary intake and behaviour in adolescent patients. The European Journal of Orthodontics, 34(4), 432-436.
  • Alajmi, S., Shaban, A., & Al-Azemi, R. (2020). Comparison of short-term oral impacts experienced by patients treated with Invisalign or conventional fixed orthodontic appliances. Medical Principles and Practice, 29(4), 382-388.
  • Behr, M., Zeman, F., Baitinger, T., Galler, J., Koller, M., Handel, G., & Rosentritt, M. (2014). The clinical performance of porcelain-fused-to-metal precious alloy single crowns: chipping, recurrent caries, periodontitis, and loss of retention. International Journal of Prosthodontics, 27(2).
  • Cha, S., Zhang, C., & Zhao, Q. (2020). Treatment of Class II malocclusion with tooth movement through the maxillary sinus. American Journal of Orthodontics and Dentofacial Orthopedics, 157(1), 105-116.
  • Ehsani, S., Nebbe, B., Normando, D., Lagravere, M. O., & Flores-Mir, C. (2015). Dental and skeletal changes in mild to moderate Class II malocclusions treated by either a Twin-block or Xbow appliance followed by full fixed orthodontic treatment. The Angle Orthodontist, 85(6), 997-1002.
  • Golden, B. A., Baldwin, C., Sherwood, C., Abdelbaky, O., Phillips, C., Offenbacher, S., & White Jr, R. P. (2015). Monitoring for periodontal inflammatory disease in the third molar region. Journal of Oral and Maxillofacial Surgery, 73(4), 595-599.
  • Guo, L., Feng, Y., Guo, H. G., Liu, B. W., & Zhang, Y. (2016). Consequences of orthodontic treatment in malocclusion patients: clinical and microbial effects in adults and children. BMC oral health, 16(1), 1-7.
  • Guo, L., Feng, Y., Guo, H. G., Liu, B. W., & Zhang, Y. (2016). Consequences of orthodontic treatment in malocclusion patients: clinical and microbial effects in adults and children. BMC oral health, 16(1), 1-7.
  • Guo, R., Lin, Y., Zheng, Y., & Li, W. (2017). The microbial changes in subgingival plaques of orthodontic patients: a systematic review and meta-analysis of clinical trials. BMC Oral Health, 17(1), 90.
  • Kim, S. H., Choi, D. S., Jang, I., Cha, B. K., Jost-Brinkmann, P. G., & Song, J. S. (2012). Microbiologic changes in subgingival plaque before and during the early period of orthodontic treatment. The Angle orthodontist, 82(2), 254-260.
  • Liberati, A., Altman, D. G., Tetzlaff, J., Mulrow, C., Gøtzsche, P. C., Ioannidis, J. P.,... & Moher, D. (2009). The PRISMA statement for reporting systematic reviews and meta-analyses of studies that evaluate health care interventions: explanation and elaboration. Journal of clinical epidemiology, 62(10), e1-e34.
  • Liu, P., Liu, Y., Wang, J., Guo, Y., Zhang, Y., & Xiao, S. (2014). Detection of fusobacterium nucleatum and fadA adhesin gene in patients with orthodontic gingivitis and non-orthodontic periodontal inflammation. PLoS One, 9(1), e85280.
  • Mártha, K., Lőrinczi, L., Bică, C., Gyergyay, R., Petcu, B., & Lazăr, L. (2016). Assessment of periodontopathogens in subgingival biofilm of banded and bonded molars in early phase of fixed orthodontic treatment. Acta Microbiologica et Immunologica Hungarica, 63(1), 103-113.
  • Mummolo, S., Tieri, M., Nota, A., Caruso, S., Darvizeh, A., Albani, F.,... & Tecco, S. (2020). Salivary concentrations of Streptococcus mutans and Lactobacilli during an orthodontic treatment. An observational study comparing fixed and removable orthodontic appliances. Clinical and Experimental Dental Research, 6(2), 181-187.
  • Pan, S., Liu, Y., Si, Y., Zhang, Q., Wang, L., Liu, J.,... & Xiao, S. (2017). Prevalence of fimA genotypes of Porphyromonas gingivalis in adolescent orthodontic patients. Plos one, 12(11), e0188420.
  • Potârnichie, O., Solomon, S., Păsărin, L., Mârțu, A., Ursărescu, I., Niţescu, D. C., & Mârțu, S. (2013). Statistical study regarding the prevalence of the periodontal pathology on the teenager patient. Romanian J Oral Rehabilit, 5(2), 80.
  • Rafiei, M., Kiani, F., Sayehmiri, F., Sayehmiri, K., Sheikhi, A., & Azodi, M. Z. (2017). Study of Porphyromonas gingivalis in periodontal diseases: A systematic review and meta-analysis. Medical journal of the Islamic Republic of Iran, 31, 62.
  • Ren, W., Yang, L., Du, F., Huang, J., Huang, Z., Liang, T.,... & Guo, L. (2020). Chipped porcelain-fused-to-metal restoration repaired by the novel self-glazed zirconia veneering with a digital workflow. Advances in Applied Ceramics, 1-6.
  • Rodrigues, R. S., Silveira, V. R., & Rego, R. O. (2020). Analysis of Porphyromonas gingivalis fimA genotypes in severe periodontitis patients. Brazilian Oral Research, 34.
  • Rosa, E. P., Murakami-Malaquias-Silva, F., Schalch, T. O., Teixeira, D. B., Horliana, R. F., Tortamano, A.,... & Bussadori, S. K. (2020). Efficacy of photodynamic therapy and periodontal treatment in patients with gingivitis and fixed orthodontic appliances: Protocol of randomized, controlled, double-blind study. Medicine, 99(14), e19429.
  • Takahashi, N., & Nyvad, B. (2011). The role of bacteria in the caries process: ecological perspectives. Journal of dental research, 90(3), 294-303.
  • Tefiku, U., Popovska, M., Cana, A., Zendeli-Bedxeti, L., Recica, B., Spasovska-Gjorgovska, A., & Spasovski, S. (2020). Determination of the Role of Fusobacterium Nucleatum in the Pathogenesis in and Out the Mouth. prilozi, 41(1), 87-99.
  • Yang, N. Y., Zhang, Q., Li, J. L., Yang, S. H., & Shi, Q. (2014). Progression of periodontal inflammation in adolescents is associated with increased number of P orphyromonas gingivalis, P revotella intermedia, T annerella forsythensis, and F usobacterium nucleatum. International journal of paediatric dentistry, 24(3), 226-233.
  • Zou, J., Meng, M., Law, C. S., Rao, Y., & Zhou, X. (2018). Common dental diseases in children and malocclusion. International journal of oral science, 10(1), 1-7.


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