Eurasian Journal of Biosciences


Background and aim: laser treatment protocols are challenging and the most effective method with desirable and excellent results is controversial, so the present study aims to evaluate the efficacy of laser therapy in root canal disinfection with different irrigants protocols.
Method: From the electronic databases, PubMed, Cochrane Library, Embase, ISI have been used to perform a systematic literature between 2010 and 2020. Therefore, a software program (Endnote X8) has been utilized for managing the electronic titles. Searches were performed with mesh terms.
Result: A total of 26 potentially relevant titles and abstracts were found during the electronic and manual search. Finally, a total of five publications fulfilled the inclusion criteria required for this systematic review. Mean difference of photodynamic therapy and control group was (MD, -3.29 95% CI -3.80, -2.79. P= 0.00) among five studies
Conclusion: The results show that the use of antimicrobial photodynamic therapy can be considered as an alternative to conventional treatment.


  • Asnaashari, M., Ashraf, H., Rahmati, A., & Amini, N. (2017). A comparison between effect of photodynamic therapy by LED and calcium hydroxide therapy for root canal disinfection against Enterococcus faecalis: A randomized controlled trial. Photodiagnosis and photodynamic therapy, 17, 226-232.
  • Bordea, I. R., Hanna, R., Chiniforush, N., Grădinaru, E., Câmpian, R. S., Sîrbu, A.,... & Benedicenti, S. (2020). Evaluation of the outcome of various laser therapy applications in root canal disinfection: A systematic review. Photodiagnosis and Photodynamic Therapy, 29, 101611.
  • Camacho-Alonso, F., Julián-Belmonte, E., Chiva-García, F., & Martínez-Beneyto, Y. (2017). Bactericidal efficacy of photodynamic therapy and chitosan in root canals experimentally infected with Enterococcus faecalis: an in vitro study. Photomedicine and laser surgery, 35(4), 184-189.
  • Chiniforush, N., Pourhajibagher, M., Shahabi, S., Kosarieh, E., & Bahador, A. (2016). Can antimicrobial photodynamic therapy (aPDT) enhance the endodontic treatment?. Journal of lasers in medical sciences, 7(2), 76.
  • de Miranda, R. G., & Colombo, A. P. V. (2018). Clinical and microbiological effectiveness of photodynamic therapy on primary endodontic infections: a 6-month randomized clinical trial. Clinical oral investigations, 22(4), 1751-1761.
  • Hoedke, D., Enseleit, C., Gruner, D., Dommisch, H., Schlafer, S., Dige, I., & Bitter, K. (2018). Effect of photodynamic therapy in combination with various irrigation protocols on an endodontic multispecies biofilm ex vivo. International endodontic journal, 51, e23-e34.
  • Lee, L. W., Lee, Y. L., Hsiao, S. H., & Lin, H. P. (2017). Bacteria in the apical root canals of teeth with apical periodontitis. Journal of the Formosan Medical Association, 116(6), 448-456.
  • Marinic, K., Manoil, D., Filieri, A., Wataha, J. C., Schrenzel, J., Lange, N., & Bouillaguet, S. (2015). Repeated exposures to blue light-activated eosin Y enhance inactivation of E. faecalis biofilms, in vitro. Photodiagnosis and photodynamic therapy, 12(3), 393-400.
  • Mohammadi, Z., Jafarzadeh, H., Shalavi, S., & Kinoshita, J. I. (2017). Photodvnamic Therapy in Endodontics. The journal of contemporary dental practice, 18(6), 534-538.
  • Moher, D., Liberati, A., Tetzlaff, J., Altman, D. G., Altman, D., Antes, G.,... & Clark, J. (2009). Preferred reporting items for systematic reviews and meta-analyses: The PRISMA statement (Chinese edition). Journal of Chinese Integrative Medicine, 7(9), 889-896.
  • Moritz, A., Gutknecht, N., Schoop, U., Goharkhay, K., Doertbudak, O., & Sperr, W. (1997). Irradiation of infected root canals with a diode laser in vivo: results of microbiological examinations. Lasers in Surgery and Medicine: The Official Journal of the American Society for Laser Medicine and Surgery, 21(3), 221-226.
  • Pinheiro, S. L., Azenha, G. R., Democh, Y. M., Nunes, D. C., Provasi, S., Fontanetti, G. M.,... & da Silveira Bueno, C. E. (2016). Antimicrobial activity of photodynamic therapy against Enterococcus faecalis before and after reciprocating instrumentation in permanent molars. Photomedicine and laser surgery, 34(12), 646-651.
  • Pourhajibagher, M., Chiniforush, N., Raoofian, R., Pourakbari, B., Ghorbanzadeh, R., Bazarjani, F., & Bahador, A. (2016). Evaluation of photo-activated disinfection effectiveness with methylene blue against Porphyromonas gingivalis involved in endodontic infection: an in vitro study. Photodiagnosis and photodynamic therapy, 16, 132-135.
  • Pourhajibagher, M., Ghorbanzadeh, R., Parker, S., Chiniforush, N., & Bahador, A. (2017). The evaluation of cultivable microbiota profile in patients with secondary endodontic infection before and after photo-activated disinfection. Photodiagnosis and Photodynamic Therapy, 18, 198-203.
  • Prażmo, E. J., Godlewska, R. A., & Mielczarek, A. B. (2017). Effectiveness of repeated photodynamic therapy in the elimination of intracanal Enterococcus faecalis biofilm: an in vitro study. Lasers in Medical Science, 32(3), 655-661.
  • Qi, M., Chi, M., Sun, X., Xie, X., Weir, M. D., Oates, T. W.,... & Xu, H. H. (2019). Novel nanomaterial-based antibacterial photodynamic therapies to combat oral bacterial biofilms and infectious diseases. International journal of nanomedicine, 14, 6937.
  • Shehab, N. F., Al-Sabawi, N. A., & Alkhalidi, E. F. (2020). Influence of an 810-nm diode laser on the temperature changes of the external root surface: An in vitro study. Journal of International Society of Preventive and Community Dentistry, 10(4), 445.
  • Siqueira Jr, J. F. (2001). Aetiology of root canal treatment failure: why well‐treated teeth can fail. International endodontic journal, 34(1), 1-10.
  • Susila, A. V., Sugumar, R., Chandana, C. S., & Subbarao, C. V. (2016). Combined effects of photodynamic therapy and irrigants in disinfection of root canals. Journal of biophotonics, 9(6), 603-609.
  • Tabassum, S., & Khan, F. R. (2016). Failure of endodontic treatment: The usual suspects. European journal of dentistry, 10(1), 144.
  • Tennert, C., Drews, A. M., Walther, V., Altenburger, M. J., Karygianni, L., Wrbas, K. T.,... & Al-Ahmad, A. (2015). Ultrasonic activation and chemical modification of photosensitizers enhances the effects of photodynamic therapy against Enterococcus faecalis root-canal isolates. Photodiagnosis and photodynamic therapy, 12(2), 244-251.
  • Tennert, C., Feldmann, K., Haamann, E., Al-Ahmad, A., Follo, M., Wrbas, K. T.,... & Altenburger, M. J. (2014). Effect of photodynamic therapy (PDT) on Enterococcus faecalis biofilm in experimental primary and secondary endodontic infections. BMC oral health, 14(1), 1-8.
  • Trindade, A. C., De Figueiredo, J. A. P., Steier, L., & Weber, J. B. B. (2015). Photodynamic therapy in endodontics: a literature review. Photomedicine and laser surgery, 33(3), 175-182.


This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.