Surface water contamination remains a major worldwide public health concern and may contribute to the dissemination of antibiotic-resistant bacteria. The current study dealt with the assessment of physicochemical parameters in the wastewater treatment plant in Almamierh, in the city of Babylon Province, Iraq. The treated water in this plant is used for agricultural purposes. To evaluate water quality, samples were collected from three sites (input, sedimentation, output) for estimation physicochemical parameters that includes (Water temperature, EC, PH, BOD, COD, TDS). the highest concentration recorded in the first site, especially BOD5 (235 Mg/L), COD (761 Mg/L), TDS (626.432 mg/L), while the EC was the highest value in the second location (1008ms/cm). These samples subjected to bacteriological analysis and a number of bacterial species have been isolated, such as: Escherichia coli, Klebsiella pneumonia, Enterococcus faecalis, pseudomonas aeruginosa. Isolates were tested for resistance to 12 classes of antibiotics, the results showed that the highest resistance levels were to β-lactam antibiotics, followed by penicillin, cephem, Fluoroquinolones, tetracycline, glycopeptides, and erythromycin.

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