The expansion of cancer disease, cardiovascular disease, diabetes, and recently COVID-19 have guided scientists toward the utilization of natural products from plants because of cheap and availability. Flavonoids one of the most important compounds extracted from the plant such as genus Stachys in this research, which illustrate that the MtOH extract of Stachys sp. has 103.36 mg of flavonoids content and also the result of scavenging ability of extract on hydrogen peroxide show the highest inhibition percentage (38%) belongs to (2 mg/ml) of extract. The result of reducing power activity illustrates high reducing activity for concentrations (10-50 µg/ml). DNA damage protection experiment showed the ability of Stachys sp. MtOH extract to protect human genomic DNA at two concentrations (2 and 1 mg/ml). Molecular docking using AutoDock 4.2 software was used to explain the binding mode between two selected flavonoids (apigenin and luteolin) in Stachy extract to help us understand the ability of flavonoids for DNA protection, results illustrate the best conformation with free binding energy (-6.86 & -7.11) for apigenin and luteolin, respectively. The results also show the formation of hydrogen bonds with the DNA (1N37) double helix.

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