Establishment of mode parameters of extraction of pumpkin pectin-containing extract by enzyme method
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2020 - Volume 14 Issue 2, pp. 4261-4269
  • Published Online: 21 Oct 2020
  • Open Access Full Text (PDF)


Almaty is the largest megapolis of Kazakhstan, located in a lowland between the mountain ranges of the Northern Tien Shan, where is atmospheric air poorly blown. Therefore, air pollution during weak natural ventilation, especially with salts of heavy metals, has a great influence on the growth of various diseases, especially the respiratory tract and cardiovascular diseases. The degree of increase of the content of heavy metals in the body of pigeons depending on their habitat was experimentally established. The total protein in the blood plasma of pigeons from ecologically safe zones of the city averaged 50 - 55 g / l. With increasing environmental pollution and the accumulation of heavy metals in the body of pigeons, there was a tendency to a decrease in total protein in the range of 35 - 39 g / l. For the correction of pathological conditions of the human body, the prevention and rehabilitation of intoxication, the development of enterosorbents is necessary, one of which is various pectin products.
Based on the conducted experiments, the regime parameters of obtaining pumpkin pectin-containing extract by the enzymatic method were established. Rational regimes for the extraction of pectin from pumpkin pomace of the Karina variety (temperature 40-41 ° C, dose of Pectinase from Aspergillus niger enzyme preparation 2.0%, medium pH 6.0, exposure time 4 hours) were established.


  • Burger, J., & Gochfeld, M. (1993). Lead and cadmium accumulation in eggs and fledgling seabirds in the New York Bight. Environmental Toxicology and Chemistry: An International Journal, 12(2), 261-267.
  • Cai, F., & Calisi, R. M. (2016). Seasons and neighborhoods of high lead toxicity in New York City: the feral pigeon as a bioindicator. Chemosphere, 161, 274-279.
  • Carocci A (2016). [id.]. Lead Toxicity, Antioxidant Defense and Environment Springer, Cham, 45–67 s.
  • Cui J(2018). [et al.]. Metal concentrations in homing pigeon lung tissue as a biomonitor of atmospheric pollution // Ecotoxicology. No 2 (27). C. 169–174.
  • Dmowski, K. (1999). Birds as bioindicators of heavy metal pollution: review and examples concerning European species. ACTA ORNITHOLOGICA-POLSKA AKADEMIA NAUK-ORIGINAL EDITION-, 34, 1-26.
  • Donchenko L.V (2018). Functional food technology: / textbook for high schools / - M. Yurayt Publishing House, 176p.
  • Elezaj I., I MehmetiAp.S., Milaimi A. Metal Bioaccumulation, Enzymatic Activity, Total Protein and Hematology of Feral Pigeon (Columba Livia), Living in the Courtyard of Ferronickel Smelter in Drenas. 2011.
  • Francisco N. De, Ruiz Troya J.D., Agüera E.I. Lead and lead toxicity in domestic and free living birds // Avian Pathology. 2003. No. 1 (32). C. 3–13.
  • Frantz A. [id.]. Contrasting levels of heavy metals in the feathers of urban pigeons from close habitats suggest limited movements at a restricted scale // Environmental Pollution. 2012. (168). C. 23–28.
  • Hoff Brait C.H., AntoniosiFilho N.R. Use of feathers of feral pigeons (Columba livia) as a technique for metal quantification and environmental monitoring // Environmental Monitoring and Assessment. 2011. No. 1–4 (179). C. 457-467.
  • Huff G.R (2008). [and etc.]. Effects of Escherichia coli challenge and transport stress on hematology and serum chemistry values of three genetic lines of turkeys // Poultry Science. No. 11 (87). C. 2234–2241.
  • Kizatova, M. Z., Alibayeva, B. N., Azimova, S. T., Iskakova, G. K., Nabiyeva, Z. S., & Uvakasova, G. T. (2019). The Impact of the Ecological Situation on Blood Parameters of Pigeons. International Journal of Psychosocial Rehabilitation, 23(1).
  • Kondratenko V.V., Kondratenko T.Yu (2019). Features of the formation of sorption properties of pectin substances from different types of pumpkin. Bulletin of the South Ural State University. Series: Food TechnologyNo. 4. S.5-12.
  • Lierz M (2003). Avian renal disease: pathogenesis, diagnosis, and therapy // The veterinary clinics of North America. Exotic animal practice. No. 1 (6). C. 29–55, v.
  • Markowski M (2013). [id.]. Avian feathers as bioindicators of the exposure to heavy metal contamination of food. // Bulletin of environmental contamination and toxicology. No. 3 (91). C. 302–5.
  • Mikheeva, A. V (2013). Isolation of pectin from plant materials and the study of its certain chemical properties // Bulletin of the Voronezh State University, series: chemistry. Biology. Pharmacy. No. 2. - P.55-62.
  • Mitra P (2017). [id.]. Clinical and molecular aspects of lead toxicity: An update // Critical Reviews in Clinical Laboratory Sciences. No. 7–8 (54). C. 506-528.
  • Nabiyeva Zh.S., Merdzhanov P., Angelova-Romova M., Zlatanov M., Antova G., Stoyanova A., Kizatova M., Karadzhov G (2013). Lipid Composition during the germination of Kazakhstan maize hybrid (Study of lipid composition Kazakhstan hybrids of corn during germination) // Bulgarian Journal of Agricultural Science, ISSN: 1310-0351, 19 (No 4) P. 780-784.
  • Nam D.-H., Lee D.-P (2006). Monitoring for Pb and Cd pollution using feral pigeons in rural, urban, and industrial environments of Korea // Science of The Total EnvironmentNo. 1-3 (357). C. 288–295.
  • Ohi G. (1974).[et al.]. The pigeon, a sensor of lead pollution // Bulletin of Environmental Contamination and ToxicologyNo. 1 (12). C. 92–98.
  • RafatiRahimzadeh M (2017). [et al.]. Cadmium toxicity and treatment: An update. // Caspian journal of internal medicine. No 3 (8). C. 135-145.
  • Rani A. [et al.]. Cellular mechanisms of cadmium-induced toxicity: a review // International Journal of Environmental Health Research. 2014. No. 4 (24). C. 378-399.
  • Russell A (2018). [id.]. A Spatial Survey of Environmental Indicators for Kazakhstan: An Examination of Current Conditions and Future Needs // International Journal of Environmental Research. No 5 (12). C. 735-748.
  • Sears M.E (2013). Chelatation: Harnessing and enhancing heavy metal detoxification-A review. // The Scientific World Journal, Vol. Articles ID 219840, 13 p.
  • Shukesheva SE. Uzakov Ya.M., Chernukha IM, Nabiyeva Zh.S., Kozybayeva A (2018). Investigation of the influence starter cultures on the acceleration ripering period finished meat products // Acta Technica, Vol. 63 - No. 1B. - R.1-8.
  • Silin V.E., Tsuglenok N.V., Tsuglenok G.I (2014). Technology for the production of pectin from squeezed red currants (Ribesrubrum) // Bulletin of the Krasnoyarsk State Agrarian University. No. 7. - P. 195-197.
  • Sobol I.V. (2016).The use of highly purified sunflower pectin in functional foods. Food Engineering and Technology, Volume 43, No. 4, P.
  • Sobol I.V., Rodionova L.Ya., Barysheva I.N (2016). Studying the possibility of obtaining high purity pectin extracts // Scientific journal KubGAU. No. 123 (09). - S. 54-59.
  • Vanamala J., Glagolenko A., Yang P., Carroll RJ, Murphy ME, Newman RA, Ford JR, Braby LA, Chapkin RS, Turner ND, Lupton JR,(2008). Dietary fish and pectin enhance colonocyte apoptosis in part through suppression of PRAR {delta} / PGE2 and elevation of PGE3.// Carcinogenesis, 29 (4), p. 790-796. PMC2659531.
  • Yang H., Shu Y. Cadmium transporters in the kidney and cadmium-induced nephrotoxicity. // International journal of molecular sciences. 2015. No. 1 (16). C. 1484–94.


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