AMA 10th edition
In-text citation: (1), (2), (3), etc.
Reference: Mateyev YZ, Shalginbayev DB, Mateyeva SZ, Ostrikov AN, Terekhina AV, Kopylov MV. Environmental study of fatty acid composition of safflower oil received by cold pressing method. Eurasia J Biosci. 2019;13(1), 385-391.

APA 6th edition
In-text citation: (Mateyev et al., 2019)
Reference: Mateyev, Y. Z., Shalginbayev, D. B., Mateyeva, S. Z., Ostrikov, A. N., Terekhina, A. V., & Kopylov, M. V. (2019). Environmental study of fatty acid composition of safflower oil received by cold pressing method. Eurasian Journal of Biosciences, 13(1), 385-391.

Chicago
In-text citation: (Mateyev et al., 2019)
Reference: Mateyev, Yesmurat Z., Daulet B. Shalginbayev, Sulushash Z. Mateyeva, Alexander N. Ostrikov, Anastasiya V. Terekhina, and Maksim V. Kopylov. "Environmental study of fatty acid composition of safflower oil received by cold pressing method". Eurasian Journal of Biosciences 2019 13 no. 1 (2019): 385-391.

Harvard
In-text citation: (Mateyev et al., 2019)
Reference: Mateyev, Y. Z., Shalginbayev, D. B., Mateyeva, S. Z., Ostrikov, A. N., Terekhina, A. V., and Kopylov, M. V. (2019). Environmental study of fatty acid composition of safflower oil received by cold pressing method. Eurasian Journal of Biosciences, 13(1), pp. 385-391.

MLA
In-text citation: (Mateyev et al., 2019)
Reference: Mateyev, Yesmurat Z. et al. "Environmental study of fatty acid composition of safflower oil received by cold pressing method". Eurasian Journal of Biosciences, vol. 13, no. 1, 2019, pp. 385-391.

Vancouver
In-text citation: (1), (2), (3), etc.
Reference: Mateyev YZ, Shalginbayev DB, Mateyeva SZ, Ostrikov AN, Terekhina AV, Kopylov MV. Environmental study of fatty acid composition of safflower oil received by cold pressing method. Eurasia J Biosci. 2019;13(1):385-91.

Abstract

Safflower is an olive culture of the sunflower family, the most widespread in the environment of arid regions of Central Asia. Its seeds contain 25-37% (in the core 46-60%) of semi-drying oils and up to 12% protein. As an object of study used safflower seeds of varieties Iyrkas. Aim of the present study was to determine the fatty acid composition of safflower oil received by cold pressing method. In order to do this, an SP-2560 column and a “Chromoteck 5000.1” gas chromatograph were used. When analyzing the chromatograms obtained in the study, the most crucial and complex stage is the identification of peaks. To determine the content of each fatty acid, the area normalization method was used. In the process of producing safflower oil in a cold way, waste such as cake is formed. In the study of the amino acid composition of safflower cake using ion-exchange chromatography with postcolumn derivatization of ninhydrid (without tryptophan), a Shimadzu LC-20 Prominence liquid chromatograph was used, and the determination of tryptophan by ion-exchange derivatization of ninhydrin. It is revealed that the protein of the safflower oil cake contains 18 amino acids. All amino acids that make up the safflower cake are α-amino acids. Mass fraction of essential amino acids in safflower oil was 5.66%, in particular, lysine contains 82.15 nmol/ml, methionine 45.10 nmol/ml. The obtained values for the quality characteristics indicate the prospects of using this type of natural oil: directly for food, as well as for production of oilseeds, such as mayonnaise, sauces, spreads; as biofuel; when designing and optimizing compound feed formulations.

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