Environmental health investigation of the partial purification and study of Guanase in sera of hepatitis B and C patients
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2019 - Volume 13 Issue 1, pp. 633-638
  • Published Online: 15 May 2019
  • Article Views: 63 | Article Download: 111
  • Open Access Full Text (PDF)
AMA 10th edition
In-text citation: (1), (2), (3), etc.
Reference: Rasheed MN, Ali SJ. Environmental health investigation of the partial purification and study of Guanase in sera of hepatitis B and C patients. Eurasia J Biosci. 2019;13(1), 633-638.

APA 6th edition
In-text citation: (Rasheed & Ali, 2019)
Reference: Rasheed, M. N., & Ali, S. J. (2019). Environmental health investigation of the partial purification and study of Guanase in sera of hepatitis B and C patients. Eurasian Journal of Biosciences, 13(1), 633-638.

Chicago
In-text citation: (Rasheed and Ali, 2019)
Reference: Rasheed, Mohammed N., and Susan J. Ali. "Environmental health investigation of the partial purification and study of Guanase in sera of hepatitis B and C patients". Eurasian Journal of Biosciences 2019 13 no. 1 (2019): 633-638.

Harvard
In-text citation: (Rasheed and Ali, 2019)
Reference: Rasheed, M. N., and Ali, S. J. (2019). Environmental health investigation of the partial purification and study of Guanase in sera of hepatitis B and C patients. Eurasian Journal of Biosciences, 13(1), pp. 633-638.

MLA
In-text citation: (Rasheed and Ali, 2019)
Reference: Rasheed, Mohammed N. et al. "Environmental health investigation of the partial purification and study of Guanase in sera of hepatitis B and C patients". Eurasian Journal of Biosciences, vol. 13, no. 1, 2019, pp. 633-638.

Vancouver
In-text citation: (1), (2), (3), etc.
Reference: Rasheed MN, Ali SJ. Environmental health investigation of the partial purification and study of Guanase in sera of hepatitis B and C patients. Eurasia J Biosci. 2019;13(1):633-8.

Abstract

Viral hepatitis is one of the most common type of diseases in the world, it is associated with high risk of cirrhosis and liver cancer, the objective of this study is to evaluate the efficacy of enzyme guanase and purifying enzyme in patients with hepatitis B and C virus. The study included 63 hepatitis B and C patients, it also included 28 people who had no infection and a similar age for patients where it was considered a control group, the age group ranged from 25-55 years for each group of patients and control, measure the level of guanase enzyme in both the patients and control group, the study also included purification of the enzyme from hepatitis B and C patients using ammonium sulphate, ion exchange technology and gel filtering technology, using DEAE-Cellulose and Sepharose-6B, samples were collected between April 2018 and July 2018 at Kirkuk general hospital. The results showed that there was significant increase (P 0.05) in the level of guanase activity in hepatitis B patient compared with control group and a highly significant increase (P 0.05) in the level of guanase activity in hepatitis C patient compared with control group. There was no difference in guanase activity in serum of HBV and HCV both in male and female the present study included partial purification of guanase from serum of hepatitis B and C patients using salt precipitation, ion exchange chromatography and gel filtration by using DEAE- Cellulose and sepharose-6B,the purification degree of HBV and HCV by ammonium sulfate precipitation was(4.358) fold, while it was by Ion exchange (8.939) fold and by gel filtration (39.446) fold, while enzyme yield was (64.084%,50.5%, 41.535%) respectively, and specific activity was (226.179,463.914, 8921,99) pg/ml, also this study included the determination of molecular weight of the purified guanase in serum of HBV and HCV by using standard plot of proteins, the results showed that molecular weight was (71.131KD), the study concluded that guanase can be considered as a predictive function in diagnosis and follow-up of viral hepatitis before and after infection.

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