Effect of the protein mixture based on dry mare’s milk on the state of antioxidant defense system and lipid peroxidation of membranes under small doses of lead affecting a body

Abstract

The article reviews the state of antioxidant system, in the setting of exposure to lead acetate, the daily consumption of a protein mixture based on mare’s milk has contributed to increased urinary excretion of lead and a decrease in its level in blood plasma.
Also, on the 60th day of the protein mixture intake, a positive dynamics of antioxidant status was noted, expressed in a decrease in the MDA and diene conjugates level in the blood red-cells membranes compared to the control variant by 56.4% and 43.75%, respectively, as well as an increase in the total antioxidant activity by 34.35%, the activity of key enzymes of the antioxidant system – superoxide dismutase by 63.37% and catalase by 89.58%. A similar pattern was observed in liver microsomes: on the 60th day of the protein mixture intake, the MDA level has decreased from (0.52±0.03) nmol/mg of protein to (0.28±0.01) nmol/mg of protein, diene conjugates – from (1.6±0.8) nmol/mg of protein to (0.85±0.05) nmol/mg of protein, and the total antioxidant activity, the activity of superoxide dismutase and catalase have increased by 17.48%, 11.34 nmol/mg of protein and 9.75 nmol/mg protein, respectively. 
The beneficial effect of the protein mixture on the phospholipid composition of membranes and decrease in their viscosity and stiffness was noted in the setting of toxic exposure to lead acetate.
The changes justify the feasibility of using the protein mixture as a means of reducing the toxic load of lead compounds on the body.
 

References

  • Abushama HM, Abdelwahab MI, Elhassan AM, Osman OF, Mohamed HSE (2014) Antibody Response to Cutaneous Leishmaniasis in Sudan. The International Journal of Biotechnology, 3(10): 123-137.
  • Astashkina AP (2010) Modern views on the biological role of bifidobacteria and lactobacilli. Bulletin of Voronezh State University, 1: 133-139.
  • Buzoeva MR, Ahpolova VO (2019) The combined effect of zinc and cadmium on the functional state of the kidneys, lipid peroxidation and calcium metabolism in experiments on rats. Bulletin of new medical technologies, 6: 127-133.
  • Carmen R, Juan C (2004) Regulation of antioxidant enzymes: a significant role for melatonin. Journal of Pineal Research, 36: 1-9.
  • Cengiz S, Mammadov R, Aykut C, Taştelen G (2015) Variations in antioxidant enzyme levels of rats exposed to ethanol extracts of Convolvulus species. Idustrial Grops and Products, 74: 304-308.
  • Dodge J, Mitchell C, Hanahan D (1963) The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes. Arch. Biochem. Biophys., 100(1): 119-130.
  • Droge W (2002) Free Radicals in the Physiological Control of Cell Function. Physiol. Rev., 82: 47-95.
  • Duggan Cr, Gannon J, Walker WA (2002) Protective nutrients and functional foods for the gastrointestinal tract. American Journal of Clinical Nutrition, 75(5): 789-808.
  • European Convention for the Protection of Vertebrate Animals Used for Experimental and other Scientific Purposes. ETS No. 123. – Strasbourg, March 18, (1986).
  • Flora G, Gupra D, Tiwari A (2012) Toxicity of lead: a reviewwith recent updates. Interdiscip. Toxicol., 5(2): 47-58.
  • Fortmann SP, Burda BU, Senger CA, Lin JS, Whitlock EP (2013) Vitamin and mineral supplements in the primary prevention of cardiovasculardisease and cancer: an updated systematic evidence review for the U.S. Ann. Intern. Med., 159: 824-834.
  • Friedman M, Brandon DL (2001) Nutritional and Health Benefits of Soy Proteins. J. Agric. Food Chem., 49(3): 1069-1086.
  • Gavrilov VB, Mishkorudnaya MI (1983) Spectrophotometric assay of the blood plasma lipid hydroperoxides. Laboratory science, 3:33-36.
  • Harabawy AS, Mosleh YY (2014) The role of vitamins A, C, E and selenium as antioxidants against genotoxicity and cytotoxicity of cadmium, copper, lead and zinc on erythrocytes of Nile tilapia, Oreochromis niloticus. Ecotoxicol. Environ. Sar., 104: 28-35.
  • Karaseva NV (2009) Development of technology and merchandise assessment of a blown finishing semi-finished product based on vegetable cream with fruit and berry fillings. abstract of a candidate of technical sciences. – Kemerovo: Karavay. – 20 p.
  • Kazenov AM, Maslova MN, Shalabodov AD (1984) Study of the activity of Na/K-ATPase in mammalian red blood cells. Biochemistry, 49(7): 1089-1095.
  • Knut J (2001) Heller Probiotic bacteria in fermented foods; product characteristics and starter organisms tract. American Journal of Clinical Nutrition, 73(2): 374-379.
  • Lowry OH, Roberts NR, Leiner KY, Wu ML, Farr AL, Albers RW (1954) The Quantitative histochemistry of brain. Iii. Ammon’s horn. J. Biol. Chem., 207(1): 39-49.
  • Mousa HM¸Al-Qarawi AA, Ali BH, Abdel Rahman HA, ElMougy SA (2002) Effect of lead exposure on the erythrocytic antioxidant levels in goats. J. Vet. Med. A Physiol. Pathol. Clin. Med., 49(10): 531-534.
  • Nam HK, Young YH, Kyu-Beck L (2015) Environmental heavy metal and chronic kidney disease in the general population. Journal of Korean medical science, 30(3): 272-277.
  • Namazbaeva ZI, Mukashev MA, Pudov AM (2012) Guidelines: Determination of heavy metals in biological material on the analytical voltammetric complex “STA”. – 16 p.
  • Netrebenko OK (2004) Revisiting the choice of therapeutic mixtures for feeding sick children. Questions of modern pediatrics, 3(1): 73-75.
  • Nouioui M, Nouioui A, Araoud M, Milliand M (2019) Biomonitoring chronic lead exposure among battery manufacturing workers in Tunisia. Environ. Sci. Pollut. Res. Int., 26(8): 7980-7993.
  • Ohkawa HO, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem., 95(2): 351-358.
  • Omura T, Sato R (1964) The carbon monoxide-binding pigment of liver microsomes. Journal of Biological Chemistry, 239(7): 2370-2378.
  • Order of the Minister of Health of the Republic of Kazakhstan. On approval of the Rules for preclinical (nonclinical) studies, clinical trials, clinical and laboratory tests of medical devices for in vitro diagnostics, as well as requirements to preclinical and clinical bases: approved April 02, 2018, № 142.
  • Placer DrZ (1968) Lipoperoxydations systeme im biologischen Material 2. Mitt. Bestimmung der Lipoperoxydation im Säugetierorganismus. Molecular nutrition, 12(6): 679-684.
  • Promyslov MSh¸ Demhcuk ML (1990) Determination of total antioxidant activity in blood serum. Medical chemistry issues, 4: 27-29.
  • Rogozhin VV, Kurilyuk TT, Kershengolts BM (n.d.) Patent of the Russian Federation No. 2112241 – Method for determining the concentration of malondialdehyde using thiobarbituric acid. – 199.
  • Rokicki W, et al. (2003) Antioxidant status in newborns and infants suferring from congenital heart defects. Wiad Lek., 56(8): 337-340.
  • Salamon RV, Salamon Sz, Csapo-Kiss Zs, Csapo J (2009) Composition of mare’s colostrum and milk. Fat content, fatty acid composition and vitamin contents. Acta Univ. Sapientiae, Alimentaria, 2: 119-131.
  • Shilina NM, Kon’ IYa (2003) The role of nutrients in the functioning of the body’s antioxidant defense system. Child Nutrition Issues, 1(4): 53-57.
  • Sizensov AN (2012) The use of probiotics against lead intoxication. Bulletin of veterinary, 63(4): 147-148.
  • Sorokina IV, Krysin AP, Khlebnikova TB, Kobrin DS, Popova LY (1997) The role of phenolic antioxidants in improving the resistance of organic systems to free radical oxidation: an analytical review. Novosibirsk. 68 p.
  • Starodumov VL, Kalinina NG, Gorbynov VA (2013) The state of red-cell membranes as an indicator of exposure to lead from environment. Bulletin of the Ivanovo Medical Academy, 18(4): 16-19.
  • Voronina, Ye.A. (2017). “Biochemical effects of cadmium and lead in food on human health”, Modern innovation, 6(20): 35-38.
  • Wang L, Wang H, Li J, et al. (2011) Simultaneous effects of lead and cadmium on primary cultures of rat proximal tubular cells: interaction of apoptosis and oxidative stress. Arch. Environ. Contam. Toxicol., 61(3): 500-511.
  • Zinchuk VV (2014) Physiological basis of nutrition. Journal of Grodno State Medical University, 3: 140-143.

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