Effect of Spirulina platensis extract on Vascular Endothelial Growth Factor (VEGF) expression in corneal inflammation in rat (Rattus novergicus) strain wistar
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2019 - Volume 13 Issue 2, pp. 823-829
  • Published Online: 07 Jul 2019
  • Article Views: 381 | Article Download: 174
  • Open Access Full Text (PDF)
AMA 10th edition
In-text citation: (1), (2), (3), etc.
Reference: Aldina R, Haryati SW. Effect of Spirulina platensis extract on Vascular Endothelial Growth Factor (VEGF) expression in corneal inflammation in rat (Rattus novergicus) strain wistar. Eurasia J Biosci. 2019;13(2), 823-829.

APA 6th edition
In-text citation: (Aldina & Haryati, 2019)
Reference: Aldina, R., & Haryati, S. W. (2019). Effect of Spirulina platensis extract on Vascular Endothelial Growth Factor (VEGF) expression in corneal inflammation in rat (Rattus novergicus) strain wistar. Eurasian Journal of Biosciences, 13(2), 823-829.

Chicago
In-text citation: (Aldina and Haryati, 2019)
Reference: Aldina, Rosy, and Shanti Widya Haryati. "Effect of Spirulina platensis extract on Vascular Endothelial Growth Factor (VEGF) expression in corneal inflammation in rat (Rattus novergicus) strain wistar". Eurasian Journal of Biosciences 2019 13 no. 2 (2019): 823-829.

Harvard
In-text citation: (Aldina and Haryati, 2019)
Reference: Aldina, R., and Haryati, S. W. (2019). Effect of Spirulina platensis extract on Vascular Endothelial Growth Factor (VEGF) expression in corneal inflammation in rat (Rattus novergicus) strain wistar. Eurasian Journal of Biosciences, 13(2), pp. 823-829.

MLA
In-text citation: (Aldina and Haryati, 2019)
Reference: Aldina, Rosy et al. "Effect of Spirulina platensis extract on Vascular Endothelial Growth Factor (VEGF) expression in corneal inflammation in rat (Rattus novergicus) strain wistar". Eurasian Journal of Biosciences, vol. 13, no. 2, 2019, pp. 823-829.

Vancouver
In-text citation: (1), (2), (3), etc.
Reference: Aldina R, Haryati SW. Effect of Spirulina platensis extract on Vascular Endothelial Growth Factor (VEGF) expression in corneal inflammation in rat (Rattus novergicus) strain wistar. Eurasia J Biosci. 2019;13(2):823-9.

Abstract

Background: Spirulina is a microalga known as has anti-inflammatory, anti-angiogenic, antioxidant, antibacterial and radioprotective properties. The aim of this study was to elucidate the differences in VEGF expression in response to various dosages of Spirulina platensis (SP) topical treatment in normal and corneal inflammation in the rat.
Material and Methods: This was an experimental study. SP powdered extract was obtained by maceration method using water as a solvent. Effect on VEGF expression was analyzed after SP aqueous extract topical treatment in corneal inflammation rat model for four times a day, over seven days. Corneal inflammation was induced by basic chemical trauma using 1N NaOH. VEGF expression was analyzed by histopathology: cornea samples were made into microscope slides and stained with immunofluorescent stain. Quantification was aided with confocal laser microscopy.
Results: Significant differences of VEGF expression were observed after topical treatment with 50 µg/mL, 100 µg/mL, and 200 µg/mL dosages. A significant correlation was also observed between SP treatment dosages and VEGF expression.
Conclusion: Topical SP treatments at 50 µg/mL, 100 µg/mL, and 200 µg/mL concentrations have anti-angiogenic effects by reducing VEGF expression in the cornea inflammation model in rats.

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