Effect of Mitomycin C on human tenon fibroblast proliferation in contracted socket tissue
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2019 - Volume 13 Issue 2, pp. 807-810
  • Published Online: 07 Jul 2019
  • Article Views: 22 | Article Download: 17
  • Open Access Full Text (PDF)
AMA 10th edition
In-text citation: (1), (2), (3), etc.
Reference: Metita M, Dewi DS, Sujuti H. Effect of Mitomycin C on human tenon fibroblast proliferation in contracted socket tissue. Eurasia J Biosci. 2019;13(2), 807-810.

APA 6th edition
In-text citation: (Metita et al., 2019)
Reference: Metita, M., Dewi, D. S., & Sujuti, H. (2019). Effect of Mitomycin C on human tenon fibroblast proliferation in contracted socket tissue. Eurasian Journal of Biosciences, 13(2), 807-810.

Chicago
In-text citation: (Metita et al., 2019)
Reference: Metita, Mirza, Debby Shintiya Dewi, and Hidayat Sujuti. "Effect of Mitomycin C on human tenon fibroblast proliferation in contracted socket tissue". Eurasian Journal of Biosciences 2019 13 no. 2 (2019): 807-810.

Harvard
In-text citation: (Metita et al., 2019)
Reference: Metita, M., Dewi, D. S., and Sujuti, H. (2019). Effect of Mitomycin C on human tenon fibroblast proliferation in contracted socket tissue. Eurasian Journal of Biosciences, 13(2), pp. 807-810.

MLA
In-text citation: (Metita et al., 2019)
Reference: Metita, Mirza et al. "Effect of Mitomycin C on human tenon fibroblast proliferation in contracted socket tissue". Eurasian Journal of Biosciences, vol. 13, no. 2, 2019, pp. 807-810.

Vancouver
In-text citation: (1), (2), (3), etc.
Reference: Metita M, Dewi DS, Sujuti H. Effect of Mitomycin C on human tenon fibroblast proliferation in contracted socket tissue. Eurasia J Biosci. 2019;13(2):807-10.

Abstract

Background: This study aimed to evaluate the effects of mitomycin C on human tenon fibroblast proliferation in contracted socket tissue.
Material and Methods: Human tenon fibroblasts from a patient who underwent socket reconstruction surgery were cultured in vitro. Cell cultures were exposed to 0.2, 0.4, and 1 mg/ml of mitomycin C solution for 5 min, and serum-free culture medium was used as a control. Proliferation was observed with the MTT cell proliferation assay 24 h after treatment.
Results: A 5 min exposure to mitomycin C at 0.2, 0.4, and 1 mg/ml caused inhibition of fibroblast proliferation (p < 0.05). The dose of mitomycin C was not significantly related to the rate of fibroblast proliferation inhibition. Mitomycin C 0.4 mg/ml caused the greatest inhibition of fibroblast proliferation compared with doses of 0.2 and 1 mg/ml.
Conclusion: Mitomycin C can inhibit tenon fibroblast proliferation of contracted socket tissue. Dose escalation of mitomycin C is not related to the rate of fibroblast proliferation inhibition.

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