Effect of lead on the viability of red blood cells and amniotic fluid cells of white rats

Abstract

The unfavorable ecological state of the environment at present is as a result of man-made human activity. Among the main pollutants of the habitat at present time is recognized the lead, which has a polytropic effect on the organism. With the help of morphometric and statistical methods, the viability has been explored of red blood cells and amniotic fluid cells of control and experimental animals which are subjected to lead acetate toxicity. To identify the belonging of amniotic fluid cells to stem cells (SC), we carried out their cultivation in the special environment «Amniokar» of the company LLC PanEco Company, Russia. Studies have been carried out using an Axio Imager.M2 digital microscope (ZEISS, Japan) and a Countess ™ automatic cell counter (Invitrogen, USA). The effect of lead acetate led to the formation of aggregates of erythrocytes of blood of the type of «coin columns», to decrease in the concentration of living erythrocytes and their viability. The study of amniotic fluid under intoxication conditions has showed that only the squamous epithelium cells remained in the suspension which has been obtained as a result of centrifugation kept squamous cells only. Lead acetate leads to a decrease in the number of viable red blood cells in the blood, as well as to an increase in their aggregation properties.

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