Effect of adding different levels of crushed laurel leaves (Laurus nobilis) to the diet on some blood parameters for broiler chickens


This experiment was conducted in the field of Poultry birds belonging to Department of Animal Production, College of Agriculture, Al-Qasim Green University in order to study the effect of adding different levels of crushed laurel leaves (Laurus nobilis) to the diet of broiler chickens on some physiological traits for blood. A 180 unsexed broiler chickens (ross 380 strain), with one-day age were used, where they were randomly divided into four treatments, with a rate of 45 birds per treatment and each treatment consisted of three replicates (15 birds per replicate). The treatments of experiment were as follows: First treatment (control) without adding crushed laurel leaves to the diet, second treatment: Adding crushed laurel leaves with amount of (1 g / kg feed), third treatment: Adding crushed laurel leaves with amount of (2 g / kg feed) and treatment Fourth: Adding crushed laurel leaves with amount of (3 g / kg feed). The experiment included the following traits: Red blood cell count, white blood cell count, Haematocrit, hemoglobin concentration, White Blood Cell Differential Count, estimating the mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and the mean corpuscular hemoglobin concentration (MCHC). The results indicated that adding laurel leaves with the rate of (2 and 3 g / kg feed) led to significant improvement (p <0.05) in the red and white blood cell counts, hemoglobin concentration, Haematocrit, the value of mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC), a significant decrease (p <0.05) in the Heterophil/lymphocyte ratio (H/L ratio). From the present experiment, it is concluded that adding laurel leaves with the rate of (2, 3 g / kg feed) to the diet can lead to improve some blood parameters for broiler chickens.


  • Adeyeye, A. S., Akanbi, W. B., Sobola, O. O., Lamidi, W. A., & Olalekan, K. K. (2016). Comparative effect of organic and in-organic fertilizer treatment on the growth and tuberyeild of sweet potato (Ipomea batata L). International Journal of Sustainable Agricultural Research, 3(3), 54-57.
  • Al-Armoush H, Omari M (1999) Herbs in the book (medical uses therapeutic cosmetic - manufacturing) Dar Alnafees for printing, publishing, and distribution. First Edition. Beirut. Lebanon.
  • Al-Darraji HJ (1995) Studying some physiological traits and thermal resistance for broilers chickens (Fawbro) and comparing it with some commercial broilers chickens. Master Thesis, College of Agriculture, University of Baghdad.
  • Al-Hassani DH (2000) Physiology of poultry birds. Dar Al Kutub For Printing & Publishing. Baghdad.
  • Al-Naimi JH (2010) Treatment with fruit trees and shrubs. Dar Al-Kutub and Documents. Baghdad. Iraq.
  • Al-Nedawi N, Abdul Latif A (2003) Effect of adding black bean seeds (Nigella sativa L.) or its oil to the diet on some productive and physiological traits for the male of broiler chickens (Fawbro). Master Thesis. College of Agriculture. Baghdad.
  • Archer RK (1965) Haematological techniques for use on animals. Oxford Book Scientific Publications.
  • Burton RR, Guion CW (1968) The differential leukocyte blood count. Its precision and individuality in the chicken. Poultry Science 47: 1945-1949.
  • Craig WJ (1999) Health-promoting properties of common herbs. Anim. J. Clin. Nutr 70(Suppl.): 491-499.
  • Duncan BD (1955) Multiple range and multiple f-test Biometrics 11: 1-42.
  • Erturk O, Ozbucak TB, Bayrak A (2006) Antimicrobial activities of some medicinal essential oils. Herba Polonica 52(1/2): 58-66.
  • Jackie W (2003) Broiler chickens: Blanching productions and Welfare. Alberta Farm Animal Care (AFAC) association. Website: www.afac.ab.ca
  • Karaalp M, Genc N (2013) Bay Laurel (Laurus Nobilis L.) In Japanese Quails Feeding. 2. Fatty Acid Content and Oxidative Stability of Breast Meat. Bulgarian Journal of Agricultural Science 19(3): 606-610.
  • Kivcak B, Mert T (2002) Preliminary evaluation of cytotoxic properties of Laurus nobilis leaf extracts. Fitoterapia 73: 242-243.
  • Musa K, Mahfuz E, Nusret G, Metin S, Musa Y, Mehmet O (2011) Bay Laurel (Laurus nobilis L.) in Japanese quails feeding 1. Performance and egg quality parameters. Journal of Animal and Veterinary Advances 10(4): 1883-1889.
  • National Research Council (NRC) (1994) Nutrient requirement of poultry then. National Academy press. Washington. D. C. USA.
  • Natt MP, Herrick CA (1952) A New blood diluent for counting the erythrocytes and leucocytes of the chicken. Poultry Science 31: 735-738.
  • Nazia MAC, Perween T (2006) Bactericidal Activity of black pepper, bay leaf, aniseed and coriander against oral isolates. J. Pharm. Sci 19(3): 214-216.
  • Ojala T, Remes S, Haansuu P, Vuorela H, Hiltumen R, Haahtela K, Vuorela P (2000) Antimicrobil activity of some coldmarin containing herbal plants growing in Finland.J.Ethnopharmacology 73: 299-305.
  • Qureshi MA, Havenstein GB (1994) A comparison the immune performance of a1991 commercial broiler with a1957 random bred strain when typical 1957 and 1991 broiler diets. Poult. Sci 73: 312-319.
  • Rezapour-Nasrabad R (2018). Transitional care model: managing the experience of hospital at home. Electronic Journal of General Medicine, 15(5): em73. https://doi.org/10.29333/ejgm/93445
  • SAS (2010) SAS/ STAT Users Guide for Personal Computers Release 9.1 SAS. Institute Inc. Cary and N.C USA.
  • Shen PF, Patterson LT (1983) A simplified wright stain technique for routine avian blood smear staining. Poultry Science 62: 923-924.
  • Sturkie PD (1986) Avian Physiology 4th ed. New York, Heidelberg Berlin, Springer Verlage.
  • Varley H, Gowenlock AH, Bell M (1980) Practical Clinical Biochemistry. 5th ed. William Heinemann Medical Books LTD, London.


This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.