Development new multiplex reverse transcription quantitative real time polymerase reaction to detect human norovirus genogroups I and II
AMA 10th edition
In-text citation: (1), (2), (3), etc.
Reference: Mohamed NS, Dahham SN, Maaroof MN. Development new multiplex reverse transcription quantitative real time polymerase reaction to detect human norovirus genogroups I and II. Eurasia J Biosci. 2019;13(1), 333-339.

APA 6th edition
In-text citation: (Mohamed et al., 2019)
Reference: Mohamed, N. S., Dahham, S. N., & Maaroof, M. N. (2019). Development new multiplex reverse transcription quantitative real time polymerase reaction to detect human norovirus genogroups I and II. Eurasian Journal of Biosciences, 13(1), 333-339.

Chicago
In-text citation: (Mohamed et al., 2019)
Reference: Mohamed, Nadira Salman, Shaymaa Naji Dahham, and Mohammed Nadhir Maaroof. "Development new multiplex reverse transcription quantitative real time polymerase reaction to detect human norovirus genogroups I and II". Eurasian Journal of Biosciences 2019 13 no. 1 (2019): 333-339.

Harvard
In-text citation: (Mohamed et al., 2019)
Reference: Mohamed, N. S., Dahham, S. N., and Maaroof, M. N. (2019). Development new multiplex reverse transcription quantitative real time polymerase reaction to detect human norovirus genogroups I and II. Eurasian Journal of Biosciences, 13(1), pp. 333-339.

MLA
In-text citation: (Mohamed et al., 2019)
Reference: Mohamed, Nadira Salman et al. "Development new multiplex reverse transcription quantitative real time polymerase reaction to detect human norovirus genogroups I and II". Eurasian Journal of Biosciences, vol. 13, no. 1, 2019, pp. 333-339.

Vancouver
In-text citation: (1), (2), (3), etc.
Reference: Mohamed NS, Dahham SN, Maaroof MN. Development new multiplex reverse transcription quantitative real time polymerase reaction to detect human norovirus genogroups I and II. Eurasia J Biosci. 2019;13(1):333-9.

Abstract

Norovirus (NV) is considering a highly contagious virus due to the increase in the number of cases of the virus in recent years. Therefore, there is a need to develop rapid, specific and sensitive molecular detection and diagnosis methods. Using the data from the National Center of Bioinformatics Information (NCBI), the sequence of more than 1000 Norovirus genogroupII genome and 39 genome of geno group I were chosen to select a common conserve region for all genotypes within the first group and the second group suitable for the design of primers and probes. The design of the first area of the junction of Open Reading Frame one (ORF) and the open reading frame two (ORF2), and the primers and probes were tested on samples of compared to the primers and using one step reverse transcription real time PCR. The kit designed for multiplex reverse reaction possess no overlap or interaction in the components of primers and probes of the two genogroups, also it is superior on the monoplex mixture of the previous kit, Norovirus being diagnosed in 45% of samples by primers and probes designed in this study, 10% of the samples were belong to the genogroupI and 35% of the samples belong to the genogroupII in comparing with virus results that tested with monoplex previous primers and probes accounted 44% , the genogroupI represented 10% and genogroupII II represented 34% of the tested samples. The use of the multiplex kit contributes to shortening the time and the largest number of samples and reduce amount of the solutions used, more than the monoplex reaction, as it should be performed separately for each genetic group.

References

  • Al-Esami HH, AL-Ramadhan Z, Ahmed AS, Shihab Z, Farhan FK (2018) Improve the surface hardness of the blend PMMA/n-MgTiO3 to resist caries and bacteria. Electronic Journal of General Medicine, 15(6): em91. https://doi.org/10.29333/ejgm/94043
  • Class RI, Parashar UD, Estes MK (2009) Norovirus gastroenteritis. New England Journal of Medicine, 361: 1776-85. https://doi.org/10.1056/NEJMra0804575
  • Ebrahimi N, Rashidinia J (2014) Spline Collocation for Volterra-Fredholm Integro-Differential Equations. UCT Journal of Research in Science, Engineering and Technology, 2(1).
  • Farkasa T, Singha A, Françoise S, Guyaderb L, Rosac LG, Saifd L, McNeala M (2015) Multiplex real-time RT-PCR for the simultaneous detection and quantification of GI, GII and GIV noroviruses, Journal of Virological Methods, 223: 109-14. https://doi.org/10.1016/j.jviromet.2015.07.020
  • Green KY, Ando T, Balayan MS, Berke T, Clarke IN, Estes MK, Maston DO, Nakata S, Neill JD, Studdert MJ, Thiel HJ (2000) Taxonomy of the caliciviruses. J Infect, 181: 322-30. https://doi.org/10.1086/315591
  • Hall AJ, Lopman BA, Payne DC, Patel MM, Gastañaduy PA, Vinjé J, Parasha UD (2013) Norovirus Disease in the United States. J. Emerging. Infect. Dis., 19(8): 1198-1205. https://doi.org/10.3201/eid1908.130465
  • Hoehne M, Schreier E (2006) Detection of Norovirus genogroup I and II by multiplex real-time RT-PCR using a 3’-minor groove binder-DNA probe. BMC infectious diseases, 6(1): 69. https://doi.org/10.1186/1471-2334-6-69
  • Huhtia L, Blazevica V, Puustinenb L, Hemminga M, Salminena M, Vesikaria T (2014) Genetic analyses of norovirus GII.4 variants in Finnish children from 1998 to 2013 Author links open overlay Infection, Genetics and Evolution, 26: 65-71. https://doi.org/10.1016/j.meegid.2014.05.003
  • Kageyama T, Kojima S, Shinohara M, Uchida K, Fukushi S, Hoshino FB, Takeda N, Katayama K (2003) Broadly reactive and highly sensitive assay for Norwalk-like viruses based on real-time quantitative reverse transcription-PCR. J Clin Microbiol, 41: 1548-57. https://doi.org/10.1128/JCM.41.4.1548-1557.2003
  • Kageyama TM, Shinohara K, Uchida S, Fukushi FB, Hoshino S, Kojima R, Takai T, Oka N, Takeda and Katayama K (2004) Coexistence of multiple genotypes, including newly identified genotypes, in outbreaks of gastroenteritis due to Norovirus in Japan. J. Clin. Microbiol., 42: 2988-95. https://doi.org/10.1128/JCM.42.7.2988-2995.2004
  • Kinzelman J, Leittl M (2012) Validity of Composite Sampling for Enumerating E. coli from Recreational Waters by Molecular Methods (qPCR) (In) Significance of Faecal Indicators in Water – A Global Perspective, Kay and Fricker (Eds). Royal Society of Chemistry, London. 188p.
  • Lazaro DR (2010) Detection and quantification of Norovirus by real time PCR. SOP Vita l018.
  • Liu J, Kibiki G, Maro V, Maro A, Kumburu H, Swai N, Taniuchi M, Gratz J, Toney D, Kang G, Houpt E (2011) Multiplex reverse transcription PCR Luminex assay for detection and quantitation of viral agents of gastroenteritis. J Clin Virol., 50(4): 308-13. https://doi.org/10.1016/j.jcv.2010.12.009
  • Mohamed NS, Habeb KA, Nasser FG, Ahmed MA, Ali KL, Najim AT (2013) The incidence of Norovirus compared with Rotavirus in Baghdad City. IJAR, 1: 855-863.
  • Niwa S, Tsukagoshi H, Ishioka T, Sasaki Y, Yoshizumi M, Morita Y, Kimura H, Kozawa K (2014) Triplex real‐time polymerase chain reaction assay for detection and quantification of norovirus (GI and GII) and sapovirus. Microbiol Immunol., 58: 68-71. https://doi.org/10.1111/1348-0421.12107
  • Patel MM, Widdowson M, Glass GI, Akazawa K, Vinjé J, Parashar UD (2008) Systematic Literature Review of Role of Noroviruses in Sporadic Gastroenteritis. Emerg Infect Dis., 14(8): 1224–31. https://doi.org/10.3201/eid1408.071114
  • Pusch D, Oh DY, Wolf S, Dumke R, Schröter-Bobsin U, Höhne M, Röske I, Schreier E (2005) Detection of enteric viruses and bacterial indicators in German environmental waters. Archives of virology, 150(5): 929-47. https://doi.org/10.1007/s00705-004-0467-8
  • Tak AZA, Ehi Y (2018) Assessment of Neurological Diagnoses in Patients Applying to the Health Board. J Clin Exp Invest., 9(3): 126-30. https://doi.org/10.5799/jcei.458760
  • Yuan JS, Reed A, Chen F, Stewart NC (2006) Statistical analysis of real-time PCR data. BMC Bioinformatics, 7: 85. https://doi.org/10.1186/1471-2105-7-85

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