Determination of genetic diversity of grapevine fanleaf virus in vineyards of East Azerbaijan Province

Abstract

Grapevine fanleaf virus (GFLV) is one of the oldest viral diseases of vineyards and it is more common in a few provinces of Iran such as East Azerbaijan (Moghan and Maragheh) and Isfahan. The name of this virus is derived from the symptoms in the leaves of this virus-infected grape tree. In the current study, it was tried to investigate the genetic variation of GFLV in East Azerbaijan province (located in Iran). For this purpose, 310 leaf samples were randomly collected during the growing season of 2017-2018. Using specific antibodies raised against Iranian isolate of GFLV, 197 samples were found to be infected with GFLV in indirect ELISA. Nucleotide sequence identities of 87-90% were found between the coat protein region of isolates of this study and that of deposited in the GenBank previously.

References

  • Arastehfar A, Daneshnia F, Zomorodian K, Najafzadeh MJ, Khodavaisy S., Zarrinfar H, ... Salehi M (2019) Low level of antifungal resistance in Iranian isolates of Candida glabrata recovered from blood samples in a multicenter study from 2015 to 2018 and potential prognostic values of genotyping and sequencing of PDR1. Antimicrobial agents and chemotherapy 63(7): e02503-18.
  • Baid A (2016) ELISA-A mini review. Res. Rev. J. Pharm. Anal 5: 1-8.
  • Boom RCJA, Sol CJ, Salimans MM, Jansen CL, Wertheim-van Dillen PM, Van der Noordaa JPME (1990) Rapid and simple method for purification of nucleic acids. Journal of clinical microbiology 28(3): 495-503.
  • Digiaro M, Elbeaino T, Martelli GP (2017) Grapevine fanleaf virus and other Old World nepoviruses. In Grapevine Viruses: Molecular Biology, Diagnostics and Management (pp. 47-82). Springer, Cham.
  • Fattouch S, Acheche H, M’hirsi S, Mellouli L, Bejar S, Marrakchi M, Marzouki N (2005) RT-PCR–RFLP for genetic diversity analysis of Tunisian Grapevine fanleaf virus isolates in their natural host plants. Journal of virological methods 127(2): 126-132.
  • Girma, N., Mekibib, F., Fikre, A., Keneni, G., Nvpr, G. R., Gaur, P., & Ojiewo, C. O. (2017). Heterosis for Nitrogen Fixation and Seed Yield and Yield Components in Chickpea (Cicer arietinum L.). International Journal of Sustainable Agricultural Research, 4(2), 50-57.
  • Hemmer C, Djennane S, Ackerer L, Hleibieh K, Marmonier A, Gersch S, ... Gertz C (2018) Nanobody‐mediated resistance to Grapevine fanleaf virus in plants. Plant biotechnology journal 16(2): 660-671.
  • Kierzkowski D, Runions A, Vuolo F, Strauss S, Lymbouridou R, Routier-Kierzkowska AL, ... Zhang Z (2019) A growth-based framework for leaf shape development and diversity. Cell 177(6): 1405-1418.
  • Martelli GP (Ed.). (1993). Graft-transmissible diseases of grapevines: handbook for detection and diagnosis. Food & Agriculture Org.
  • Nourinezhad ZS, Shams BM, Sokhandan BN, Pazhouhandeh M (2012) Identification And Detection Of Iranian Isolates Of Grapevine Fanleaf Virus Using Green-Grafting And Rt-Pcr.
  • Osterbaan LJ, Choi J, Kenney J, Flasco M, Vigne E, Schmitt-Keichinger C, ... Fuchs M (2019) The Identity of a Single Residue of the RNA-Dependent RNA Polymerase of Grapevine Fanleaf Virus Modulates Vein Clearing in Nicotiana benthamiana. Molecular Plant-Microbe Interactions 32(7): 790-801.
  • Pourrahim R, Farzadfar S, Golnaraghi AR, Ahoonmanesh A (2007) Incidence and distributions of Grapevine fanleaf virus in north-east of Iran. Plant Pathology Journal 6(3): 254-259.
  • Rupnik-Cigoj M, Jež-Krebelj A, Castellarin SD, Trošt K, Sivilotti P, Pompe-Novak M (2018) Grapevine fanleaf virus affects grape (Vitis vinifera) berry anthocyanin content via the transcriptional regulation of anthocyanin biosynthetic genes. Functional Plant Biology 45(7): 771-782.
  • Salami SA, Ebadi A, Zamani Z, Habibi MK (2009) Incidence of Grapevine Fanleaf Virusin Iran: A Survey Study and Pro-duction of Virus-Free Material Using Meristem Culture and Thermotherapy.
  • Samaali BM, Toumi A, Hamdane AM, Kallel S (2019) Study of distribution and analysis of the transmission of Grapevine fanleaf virus in northern Tunisian vineyards. Journal of New Sciences 64: 4040-4047.
  • Schmitt-Keichinger C, Hemmer C, Berthold F, Ritzenthaler C (2017) Molecular, Cellular, and Structural Biology of Grapevine fanleaf virus. In Grapevine Viruses: Molecular Biology, Diagnostics and Management (pp. 83-107). Springer, Cham.
  • Silva G, Lecourt J, Clover GRG, Seal SE (2017) First report of Grapevine fanleaf virus infecting grapevine in the United Kingdom. New Disease Reports 36(9).
  • Sokhandan BN, Pashaei A, DOULATI BH (2011) Characterization of the full length coat protein gene of Iranian Grapevine fanleaf virus isolates, genetic variation and phylogenetic analysis.
  • Vigne E, Bergdoll M, Guyader S, Fuchs M (2004) Population structure and genetic variability within isolates of Grapevine fanleaf virus from a naturally infected vineyard in France: evidence for mixed infection and recombination. Journal of General Virology 85(8): 2435-2445.
  • Wingard SA (1928) Hosts and symptoms of ring spot, a virus disease of plants.

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