Determination of congenital cytomegalovirus infection and virus genotypes in newborn urine samples
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2019 - Volume 13 Issue 1, pp. 309-313
  • Published Online: 09 May 2019
  • Article Views: 34 | Article Download: 20
  • Open Access Full Text (PDF)
AMA 10th edition
In-text citation: (1), (2), (3), etc.
Reference: Quasim TM, Alshaikhly AWAR. Determination of congenital cytomegalovirus infection and virus genotypes in newborn urine samples. Eurasia J Biosci. 2019;13(1), 309-313.

APA 6th edition
In-text citation: (Quasim & Alshaikhly, 2019)
Reference: Quasim, T. M., & Alshaikhly, A. W. A. R. (2019). Determination of congenital cytomegalovirus infection and virus genotypes in newborn urine samples. Eurasian Journal of Biosciences, 13(1), 309-313.

Chicago
In-text citation: (Quasim and Alshaikhly, 2019)
Reference: Quasim, Tariq M., and Abdel Wahab A R. Alshaikhly. "Determination of congenital cytomegalovirus infection and virus genotypes in newborn urine samples". Eurasian Journal of Biosciences 2019 13 no. 1 (2019): 309-313.

Harvard
In-text citation: (Quasim and Alshaikhly, 2019)
Reference: Quasim, T. M., and Alshaikhly, A. W. A. R. (2019). Determination of congenital cytomegalovirus infection and virus genotypes in newborn urine samples. Eurasian Journal of Biosciences, 13(1), pp. 309-313.

MLA
In-text citation: (Quasim and Alshaikhly, 2019)
Reference: Quasim, Tariq M. et al. "Determination of congenital cytomegalovirus infection and virus genotypes in newborn urine samples". Eurasian Journal of Biosciences, vol. 13, no. 1, 2019, pp. 309-313.

Vancouver
In-text citation: (1), (2), (3), etc.
Reference: Quasim TM, Alshaikhly AWAR. Determination of congenital cytomegalovirus infection and virus genotypes in newborn urine samples. Eurasia J Biosci. 2019;13(1):309-13.

Abstract

Congenital cytomegalovirus (cCMV) infection may cause significant clinical consequences in newborns, not only at birth, but also later as neurological sequelae. It is very important to establish a strategy for rapid and accurate screening of congenital CMV infections for determining the risk factors that may threaten the newborns life. To our knowledge most of the previous studies of a CMV infection in Iraq have been based on serological and immunological tests. Our current study is one of the few molecular studies focused on the molecular diagnosis of the virus and determination of the dominant viral genotype in Baghdad province. n the recent study active CMV infection was detected in 24 samples (12%) in real time PCR with significant Ct value <35. Amplicons sequencing was indicated about twelve out of 20 (60%) recipients had CMVGT2 (gB2) genotype; 6 out of 20 (30%), recipients had CMVGT4 (gB4); 2 out of 20(10%), CMVGT1 (gB1). Our finding indicated that the most frequent genotypes were gB2 followed by GB4. Real time PCR assay was very accurate, rapid, and sensitive method of CMV early diagnosis, also M-nPCR and sequencing was a specific assay for CMVGT genotyping.

References

  • Allice T, Cerruti F, Pittaluga F, et al. (2008) Evalution of a novel real-time PCR system for cytomegalovirus DNA quantitation on whole blood and correlation with pp 65-antigen test in guiding pre-emptive antiviral treatment. J Virol Methods, 148: 9–16.
  • Zuylen JW, Hamilton ST, Naing Z, Hall B, Shand A, Rawlinsonc WD (2014) Congenital cytomegalovirus infection: Clinical presentation, epidemiology, diagnosis and prevention Med., 7(4): 140–6.
  • Rawlinson WD, Hamilton ST, van Zuylen WJ (2016) Update on treatment of cytomegalovirus infection in pregnancy and of the newborn with congenital cytomegalovirus. Curr Opin Infect Dis., 29(6): 615-24.
  • Pass RF, Arav-Boger R (2018) Maternal and fetal cytomegalovirus infection: diagnosis, management, and prevention. F1000Research, (7): 255.
  • Bate SL, Dollard SC, Cannon MJ (2010) Cytomegalovirus seroprevalence in the United States: the national health and nutrition examination surveys, 1988-2004. Clin. Infect Dis., 50: 1439–47.
  • Gindes L, Teperberg-Oikawa M, Sherman D, Pardo J, Rahav G (2008) congenital Cytomegalovirus infection following primary maternal infection in the third trimester. BJOG, 115: 830–5.
  • Fowler KB, Boppana SB (2006) Congenital Cytomegalovirus (CMV) infection and hearing deficit. J Clin Virol., 35: 226–31.
  • Kimberlin DW, Jester PM, Sanchez PJ, Ahmed A, Arav-Boger R, Michaels MG, et al. (2015) National Institute of Allergy and Infectious Diseases Collaborative Antiviral Study Group. Valganciclovir for symptomatic congenital cytomegalovirus disease. N Engl J Med., 372: 933–43.
  • Peres RMB, Costa RCR, Andrade PD, Bonon HA, Albuquerque DM, de Oliveira C (2010) Surveillance of active human cytomegalovirus infection in hematopoietic stem cell transplantation (HLA sibling identical donor): search for optimal cutoff value by real-time PCR BMC Infect Dis., 10: 147.
  • Tarragó D, Quereda C, Tenorio A (2003) Different Cytomegalovirus Glycoprotein B Genotype Distribution in Serum and Cerebrospinal Fluid Specimens Determined by a Novel Multiplex Nested PCR. J Clin Microbiol., 41(7): 2872–7.
  • Sambrook J, Fritschi EF, Maniatis T (1989) Molecular cloning: a laboratory manual, Cold Spring Harbor Laboratory Press, New York.
  • Alwan A (2011) Sera interleukin-10 and interlukin- 12 level among Iraqi aborted women infected with human cytomegalovirus .Higher Diploma thesis. Health and Medical Tachnical College. Foundation of Technical Education.
  • Mujtaba G, Khurshid A, Sharif S, Alam MM, Aamir UB, Shaukat S, Angez M, Rana MS , Umair M, Shah AA, Zaidi SZ (2016) Distribution of Cytomegalovirus Genotypes among Neonates Born to Infected Mothers in Islamabad, Pakistan. Journal pone: 0156049.
  • Pati S, Pinninti S, Novak Z, Chowdhury N, Patro R, Fowler R, Ross S (2013) Genotypic Diversity and Mixed Infection in Newborn Disease and Hearing Loss in Congenital Cytomegalovirus Infection. Pediatr Infect Dis J., 32(10): 1050–4.
  • Noor Al- Huda AA, Saeed H, El-Haboby BT, AL-Jaff SK, Alsamawi AI, Khadoom LS (2016) Seroprevalence study of toxoplasma gondii, rubella virus and human cytomegalo virus infection among bad obestetric history and primigravida pregnant women in Iraq. World J Pharm Res., 5(9): 207-15.
  • Bernard S, Wiener-Vacher S, Van Den Abbeele T, Teissier N (2015) Vestibular disorders in children with congenital cytomegalovirus infection. Pediatrics, 136: 887–95.
  • Hameed MY, Aziz IH (2016) Detection of Cytomegalovirus in Iraqi Recurrent Miscarriage women World J Pharm Pharm Sci., 5(1): 79-89.
  • Dieamant DC, Bonon SHA, Peres RMB, Costa CRC, Albuquerque DM, Miranda ECM, et al. (2013) Cytomegalovirus (CMV) genotype in allogeneic hematopoietic stem cell transplantation. BMC Infectious Diseases, (13): 310.
  • Arav-Boger R (2015) Strain Variation and Disease Severity in Congenital Cytomegalovirus Infection: In Search of a Viral Marker. Infect Dis Clin North Am., 29(3): 401–14.
  • Daiminger A, Bader U, Enders G (2005) Pre- and periconceptional primary cytomegalovirus infection: risk of vertical transmission and congenital disease. BJOG, 112(2): 166–72.
  • Buxmann H, Hamprecht K, Meyer-Wittkopf M, Friese K (2017) Primary Human Cytomegalovirus (HCMV) Infection in Pregnancy. Dtsch Arztebl Int., 114: 45–52.
  • Sneath PHA, Sokal RR (1973) Numerical Taxonomy. Freeman, San Francisco.
  • Tamura K, Nei M, Kumar S (2004) Prospects for inferring very large phylogenies by using the neighbor-joining method. Proceedings of the National Academy of Sciences (USA), 101: 11030-5.
  • Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution, 30: 2725-9.
  • Renzette N, Bhattacharjee B, Jensen JD, Gibson L, Kowalik TF (2011) Extensive genome-wide variability of human cytomegalovirus in congenitally infected infants. PLoS Pathog., 7: e1001344.
  • Nahar S, Hokama A, Iraha A, Ohira T, Kinjo T, Hirata T, Kinjo T, Parrott, Jiro Fujita GL (2018) Distribution of cytomegalovirus genotypes among ulcerative colitis patients in Okinawa, Japan. Intest Res., 16(1): 90-8.
  • Al-Baiati HM, Jabbar RN, Al-Saady AK (2014) Molecular Detection of Human Cytomegalovirus genes in infertile and breast cancer women in Baghdad province Int. J. Curr. Microbiol App Sci., 3(4): 316-322.
  • Mirzamasoumzadeh B, Mollasadeghi V (2013) Effects of osmotic stress on chlorophylee and proline different wheat cultivars. UCT Journal of Research in Science, Engineering and Technology, 1(1): 12-3.
  • Rehman HU (2015) Vitamin B12 Deficiency: Some Observations, Some Misconceptions. European Journal of General Medicine, 12(3): 261-266. https://doi.org/10.15197/ejgm.01427
  • Uçar M, Vatansever F, Tanık N, Çebiçci MA, Sütbeyaz ST, Sarp Ü, et al. (2015) The comparison of mean platelet volume and other hemogram results in patients with carpal tunnel syndrome. J Clin Exp Invest., 6(2): 154-8. https://doi.org/10.5799/ahinjs.01.2015.02.0508

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