Detection of some intestinal parasites in the wastewater of some health centers (popular clinics) in some areas of Al-Diwaniyah City, Iraq
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2020 - Volume 14 Issue 1, pp. 2115-2121
  • Published Online: 30 Jun 2020
  • Open Access Full Text (PDF)

Abstract

This study diagnosed the intestinal parasites (IPs) present in the wastewater of four health centers (popular clinics) of AL-Diwaniya city; Al-Hadara district center (C1), Al-Nahda district center (C2), Al-Iskan district center (C3), Al-Orouba district center (C4) for the period from 1/6 /2019 until 1/12-2019. Total of 140 samples were collected (35 samples per health center) and samples were examined in direct smear method with both iodine and saline to detect cysts and Formalin-ether concentration technique (FECT) to detect helminthes eggs. The total presence of parasites in the wastewater of the centers was 37.85%, where the highest contamination of wastewater was 42.7857% in Center 1 and the lowest 31.42% in the C3. The recorded intestinal parasites (IP) were Enterobius vermicularis, Entamoeba coli, Giardia lamblia, Ascaris lumbricoids in proportions of 12.14%, 2.857%, 14.28%, 8.57%, respectively, the results of comparison between the direct smear method and formalin-ether concentration technique (FECT) indicated that the proportion of the presence of parasites in those waters was 22.85%, 15% respectively. The results also showed that the use of PCR-technique the presence of intestinal parasites was 35.849%, where the gene 18s rRNA appeared in percentage (41.176%, 75%, 0% 45%) for Enterobius vermicularis, Ascaris lumbricoids, Entamoeba coli, Giardia lamblia, respectively.

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