Detection of some biochemical marker in Broncho-Aleveolar Lavage obtained from patients with respiratory diseases
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2020 - Volume 14 Issue 1, pp. 1901-1904
  • Published Online: 30 Jun 2020
  • Open Access Full Text (PDF)


The present study was carried out to Detection some biochemical markers in Broncho- Aleveolar Lavage (BA) that obtained from patients with respiratory diseases , about 39 BAL samples were obtained from patients with different respiratory diseases .All samples were negative for Acid fast Bacilli(AFB). Some biochemical markers are investigated. The results observed that leukocyte esterase (LE) is positive in 31 BAL samples (79.4%). The presence of LE will reflect the presence of pus cells inside BAL fluids. On the other hand, the samples were subjected for detection RNAase III activity. It was seen that RNAase III is observed only in 10 samples of BAL with a mean value (10.4±2.7). The presence of this enzyme means that eosinophil’s are presented in high numbers. Procalcitonin (PCT) is also investigated. It was shown that PCT hormone is ranging from 0.66-1.72 ng/ml in 26 samples, 0.13-0.48 in 7 BAL samples, and < 0.1 in 4 samples whereas 2 samples are free from any amount for PCT. The presence of PCT hormone may be used as biomarker in BAL which means that the infections are caused mainly by bacteria. Besides, Ect-5’-nucleotidse (5’-NT) is found in all BAL samples with a mean value (3.66±0.99) which is present within the normal range of this enzyme in the plasma.


  • Ahmed M, Ibrahim W, Sarafaudi S, Shahzada K, Ahmed S (2019) Diagnostic value of brocho aleveolar lavage in the subset patients with Negative Mycobacterium culture. Int.J.Inf.Dis. 82: 96-101.
  • Becze Z, Moln Z, Nos (2016) Molecular basis of procalcitonine synthesis. J. Human. Virol. Retrovirol, 3(2): 1-6.
  • Bystrom J, Amin K, Bishop-Baily D, et al. (2011) Analysing the eosinophilic Cationic protein-a clue to the function of eosinophil granulocyte.Respiratory.Res.12(10): 1-10.
  • Clogan S, Eltzchig H, Eckle T, Thompson L (2006) Physiological roles for ecto-5- nucleotidase (CD73).Purinergic Signal. 2: 351-360.
  • Court D, GAN J, Liang Y, Shaw G, et al. (2013) RNaseІІІ: Genetics and function; Structure and mechanism. Ann Rev Genetics. 47: 405-431.
  • Creamer A, Kent A, Albur M (2019) Procalcitonine in respiratory diseases. Breathe.15(4): 296-304.
  • Dixon G, Lama-Lopez A, Bincliffe O, Morely A, et al. (2017) The role of PCT in establishing the diagnosis and prognosis of pleural infections. Respir.Res.18: 30-39.
  • Girma N, Mekibib F, Fikre A, Keneni G, Nvpr GR, Gaur P, Ojiewo CO (2017) Heterosis for Nitrogen Fixation and Seed Yield and Yield Components in Chickpea (Cicer arietinum L.). International Journal of Sustainable Agricultural Research, 4(2): 50-57.
  • Heron M, Grutters J, Ten K, Hijdra D, et al. (2012) Bronch-Aleveolar Lavage cells pattern from healthy human lung. Clin. Exp. Immunol.168: 523-531.
  • Karagiorga G, Nakos G, Galiatsou E, Lekka M, et al. (2006) Biochemical parameters of Brocho-aleveolar lavage in fat embolism. Intensive Care Med. 32: 116-123.
  • Ko Y, Wu W, Hsu Dai M, et al. (2009) Serum and pleural fluid PCT in predicting bacterial infections. J. Korean Ned Sci. 24: 398-402.
  • Lee W, Chung W, Hong K, Huh J (2015) Clinical usefulness of BAL cellular analysis. Ann. Lab. Med. 35: 220-227.
  • Meyer K, Raghu G (2011) Bronchaleveolar lavage for evaluation of interistitial lung disease. Eur Respir J. 38: 761-769.
  • Meyer K, Singh K, Baughman P, Brown K, et al. (2012) The clinical utilities of BAL cellular analysis. Am J Crit Care Med: 185: 1004-1014.
  • Rohini K, Srikumar P, Saxana J, Mahesh A, Bhat S (2012) Assessment of serum calicium, phosphorous, CRP and PCT in Tb patients. Int J collaborative Res. 4: 1868-1875.
  • Schuetz P, Wirz Y, Sager R, Christ-crain M, et al. (2017) Procalcitonin to initiate or discontinue antibiotics ARTI. PMC article.
  • Singh K, Khan S, Agarwal S, et al. (2014) The performance of high volume BAL for evaluation and diagnosis of lung diseases. Ann Thorac. Surg. 93: 720-726.
  • Thomas J, Errol M (2019) Corticosterone determination I Brochoaleveolar lavage and its relationship to free and total plasma corticosterone. Annalytical Biochemistry. 567: 27-29.


This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.