Detection of some biochemical marker in Broncho-Aleveolar Lavage obtained from patients with respiratory diseases
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2020 - Volume 14 Issue 1, pp. 1901-1904
  • Published Online: 30 Jun 2020
  • Open Access Full Text (PDF)

Abstract

The present study was carried out to Detection some biochemical markers in Broncho- Aleveolar Lavage (BA) that obtained from patients with respiratory diseases , about 39 BAL samples were obtained from patients with different respiratory diseases .All samples were negative for Acid fast Bacilli(AFB). Some biochemical markers are investigated. The results observed that leukocyte esterase (LE) is positive in 31 BAL samples (79.4%). The presence of LE will reflect the presence of pus cells inside BAL fluids. On the other hand, the samples were subjected for detection RNAase III activity. It was seen that RNAase III is observed only in 10 samples of BAL with a mean value (10.4±2.7). The presence of this enzyme means that eosinophil’s are presented in high numbers. Procalcitonin (PCT) is also investigated. It was shown that PCT hormone is ranging from 0.66-1.72 ng/ml in 26 samples, 0.13-0.48 in 7 BAL samples, and < 0.1 in 4 samples whereas 2 samples are free from any amount for PCT. The presence of PCT hormone may be used as biomarker in BAL which means that the infections are caused mainly by bacteria. Besides, Ect-5’-nucleotidse (5’-NT) is found in all BAL samples with a mean value (3.66±0.99) which is present within the normal range of this enzyme in the plasma.

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