AMA 10th edition
In-text citation: (1), (2), (3), etc.
Reference: Ding J, Ma X, Ai R, et al. Detection of LPXTG protein genes on food-borne listeria monocytogenes. Eurasia J Biosci. 2019;13(2), 1201-1203.

APA 6th edition
In-text citation: (Ding et al., 2019)
Reference: Ding, J., Ma, X., Ai, R., Zhang, D., Xu, Z., Zhang, J., . . . Wang, T. (2019). Detection of LPXTG protein genes on food-borne listeria monocytogenes. Eurasian Journal of Biosciences, 13(2), 1201-1203.

Chicago
In-text citation: (Ding et al., 2019)
Reference: Ding, Jian, Xun Ma, Ridengcaicike Ai, Dan Zhang, Zhengkun Xu, Jihong Zhang, Weidong Chen, Linaer Gu, and Tao Wang. "Detection of LPXTG protein genes on food-borne listeria monocytogenes". Eurasian Journal of Biosciences 2019 13 no. 2 (2019): 1201-1203.

Harvard
In-text citation: (Ding et al., 2019)
Reference: Ding, J., Ma, X., Ai, R., Zhang, D., Xu, Z., Zhang, J., . . . Wang, T. (2019). Detection of LPXTG protein genes on food-borne listeria monocytogenes. Eurasian Journal of Biosciences, 13(2), pp. 1201-1203.

MLA
In-text citation: (Ding et al., 2019)
Reference: Ding, Jian et al. "Detection of LPXTG protein genes on food-borne listeria monocytogenes". Eurasian Journal of Biosciences, vol. 13, no. 2, 2019, pp. 1201-1203.

Vancouver
In-text citation: (1), (2), (3), etc.
Reference: Ding J, Ma X, Ai R, Zhang D, Xu Z, Zhang J, et al. Detection of LPXTG protein genes on food-borne listeria monocytogenes. Eurasia J Biosci. 2019;13(2):1201-3.

Abstract

Background: Listeria monocytogenes (Lm) is well known as one of the most important foodborne pathogens, pose a great threat to the health of human and various livestock and poultry. Lm have a variety of virulence-associated proteins that closely involved in pathogenicity on the cell wall. One type of surface proteins which have a conserved LPXTG motif at COOH-terminal cell can be cleaved by sortase and anchored peptidoglycan to present on the cell surface to play a role in virulence.
Objective: To detect the LPXTG protein genes on Food-Borne Listeria monocytogenes strains that were isolated from the different foods.
Method: 41 of primers was designed and synthesized for putative LPXTG surface protein. It was detected by PCR amplify and gel electrophoresis imaging system.
Result: The results showed that the rate of carrying LPXTG protein genes in different LM isolates are different. 12.5% Lm isolates (3/24) carrying more than 85% LPXTG protein genes. The detection rate of LPXTG protein genes are different. Among the 41 LPXTG protein genes, only 7 genes were 100% detection; 3 genes were under 20%; the Lmo1115 gene was not detected. It is of importance for studying the pathogenicity of food-borne Lm to test the distribution of LPXTG motif surface proteins.

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License

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