Detection of entamoeba histolytica and cyptosporidium parvium in drinking water of Al-diwaniyah City, Iraq


The current study was carried out to detect parasitic contamination depending on presence some intestinal protozoan such as Entamoeba histolytica (in cysts phase) and Cryptosporidium parvium (in Oocysts phase) in drinking water in some neighborhoods in the city of Al-Diwaniyah (Al-Sadr, Al- Nahdah, Al- Zahra, AL- Orouba, A- Zaytoon, Al-Furat) in the Al- Qadisiyah province, (36) samples of water collected (6-samples from each neighborhood), one sample each month at the period from the beginning of January / 2017 to the end of July/2018, method was used concentration and modified Ziehl-Neelsen. The results of the study indicate that the total percentage of water contamination was 5/36 (13.88%), also indicated that the highest percentage was recorded (33.33%) of Entamoeba histolytica-cystsin Al- Nahdah neighborhood, while the lower percentage was (16.66%) in Al-Sadr, Al- Zahra, Al- Orouba neighborhoods, the statistical analysis indicated to significant differences in the percentage of contamination between Al-Nahda and the other neighborhoods at the probability (P≤0.05).
The results of the molecular examination using the conventional polymerase chain reaction indicated that the proportion of the18S rRNA gene (501bp) responsible for the diagnosis of Entamoeba histolytica in drinking water was 80% while The molecular examination results indicated that there was no found of the 18S rRNA gene (347bp) which was responsible for d diagnosis of Cryptosporidium parvium in drinking water.


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