Detection of entamoeba histolytica and cyptosporidium parvium in drinking water of Al-diwaniyah City, Iraq

Abstract

The current study was carried out to detect parasitic contamination depending on presence some intestinal protozoan such as Entamoeba histolytica (in cysts phase) and Cryptosporidium parvium (in Oocysts phase) in drinking water in some neighborhoods in the city of Al-Diwaniyah (Al-Sadr, Al- Nahdah, Al- Zahra, AL- Orouba, A- Zaytoon, Al-Furat) in the Al- Qadisiyah province, (36) samples of water collected (6-samples from each neighborhood), one sample each month at the period from the beginning of January / 2017 to the end of July/2018, method was used concentration and modified Ziehl-Neelsen. The results of the study indicate that the total percentage of water contamination was 5/36 (13.88%), also indicated that the highest percentage was recorded (33.33%) of Entamoeba histolytica-cystsin Al- Nahdah neighborhood, while the lower percentage was (16.66%) in Al-Sadr, Al- Zahra, Al- Orouba neighborhoods, the statistical analysis indicated to significant differences in the percentage of contamination between Al-Nahda and the other neighborhoods at the probability (P≤0.05).
The results of the molecular examination using the conventional polymerase chain reaction indicated that the proportion of the18S rRNA gene (501bp) responsible for the diagnosis of Entamoeba histolytica in drinking water was 80% while The molecular examination results indicated that there was no found of the 18S rRNA gene (347bp) which was responsible for d diagnosis of Cryptosporidium parvium in drinking water.

References

  • AL-Khalidy KAH (2016) Detection of Entamoeba histolytica in patients an infected infants with diarrhea in born and children’s hospital by classic methods and Real time Polymerase Chain Reaction. Al Qadisiyah Journal of Pure Sciences, 20(2).
  • Arash H, Elham H, Mohammad JH (2015) Identification of Entamoebahistolytica by molecular method in surface water of Rasht city, Iran. Iran J Public Health, 44(2): 238-243.
  • Betancourt WQ, Rose JB (2004) Drinking water treatment processes for removal of Cryptosporidiumand Giardia. Veterinary parasitology, 126(1-2): 219-234. https://doi.org/10.1016/j.vetpar.2004.09.002
  • Briefs S (2002) Update on Cryptosporidium and Giardia in wild life [Internet]. Available at: http://www.uga.edu/scwds/topicindex.
  • Ezatollah G., Mahmoud R, Morad R (2015) Prevalence of Entamoeba histolytica/disparin drinking water in the city of Shush, Khuzestan Province in 2011. Int. J. Curr. Microbiol. App. Sci 4(2): 582-588.
  • Guy RA, Payment P, Krull UJ, Horgen PA (2003) Real-Time PCR for Quantification of Giardia and Cryptosporidium in Environmental Water Samples and Sewage. Applied and Environmental microbiology, 69(9): 5178-5185. https://doi.org/10.1128/AEM.69.9.5178-5185.2003
  • Harfoush M (2020) The Impact of Operational Conditions on Commercial Membranes using in Removing Nitrate from Drinking Water. Aquademia, 4(1): ep20014. https://doi.org/10.29333/aquademia/8225
  • Henriksen A, Pholenz JFL (1980) Staining of Cryptosopridia by modified Ziehl-Neelsens technique. acta veterinary J. Scandanavian, 22: 594-596.
  • Lake IR, Bentham G, Kovats R, Nichols GL (2005) Effects of weather and river flow on Cryptosporidiosis. Journal of water and health, 3(4): 469-74.
  • Lejeune M, Rybicka JM, Chadee K (2009) Recent discoveries in the pathogenesis andimmune response toward Entamoeba histolytica fut. Microb: 105-118.
  • Linford AS, Heriberto M, Kafelyn RG, Hanbang Z, Singh V, Willian A, Petri JR (2009) Short hairpin RNA. Mediated knoch down of protein expression in Entamoeba histolytica. J. Microb: 1035-1037.
  • Meyer EA, Jarroll EL (1980) Giardiasis.Am.J. Epidemiol.111: 1-12.
  • Miceli L (2004) Distinguishing between pathogenic and non pathogenic species of Entamoeba. Lab. Med. 35: 613-616. https://doi.org/10.1309/B81NPVAW8Y4BGY11
  • Mohsen W, Yazji S, Al-Jadayel RA (2013) Use of PCR technology in the detection of Giardia on vegetables and irrigated water in rural Damascus. Damascus University Journal of Agricultural Sciences, 29(3).
  • Moon HW, Woodmansee DB (1986) Cryptosporidiosis. J. Am. Med. Assoc. 189(6): 643-646.
  • Niazi AD (2001) Statistical analysis in medical research. Uni. Nahrein Republic of Iraq. 148.
  • Nongyao A, Kitja B, Pathana PB (2004) Effects of Pipper longum Fruits on cecalamoebiasis in mice. J. Ethnopharmacol, 91: 357-360. https://doi.org/10.1016/j.jep.2004.01.014
  • Reynolds DT, Slade R, Sykes NJ, Jonas A, Fricker CR (1999) Detection of Cryptosporidium oocysts in water: techniques for generating precise recovery data. J. of Applied microbiology, 87, 804-813. https://doi.org/10.1046/j.1365-2672.1999.00862.x
  • Soave R, Armstrong D (1986) Cryptosporidium and cryptosporidiosis. J. Infec. Dis., 8: 1012-1023.
  • Taran-Benshoshan M, Ofer N, Dalit V-O, Aharoni A, Revhun M, Nitzan Y, Nasser AM (2015) Cryptosporidium and Giardia removal by secondary and tertiary wastewater treatment. Journal of environmental science and health part a-toxic/hazardous substance & environmental engineering, 50(12): 1265-1273.
  • Thulasi K, Mohamad A, Abd M, Subashini O, Narong J, Hemah A, et al. (2016) Presence of Cryptosporidium parvum and Giardia lamblia in water samples from Southeast Asia: towards an integrated water detection system. Infectious Diseases of Poverty 5: 3. https://doi.org/10.1186/s40249-016-0095-z
  • WHO (2002) Protozoan parasites (Cryptosporidium, Giardia, Cyclospora) Guidelines for drinking water quality Addendum to the second edition of the guidelines for drinking water quality. WHO, Geneva, Switzerland.

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