Cytogenetic studies and cytotoxic effect of Ruta chalepensis extract on Swiss Albion mice and MCF-7 tumor cells

Abstract

Ruta chalepensis is a native herb of the Mediterranean region used in the traditional medicine of many countries. Phytochemical screening has shown the presence of bioactive molecules that are responsible for its pharmacological properties. The present study aimed to investigate the effect of R. chalepensis methanolic extract on metaphase index and micronucleus formation in bone marrow of Swiss albino BALB/c male mice as well as in vitro cytotoxic effect against MCF-7 tumor cell line. The results of cytogenetic evaluations indicated that a treatment with R. chalepensis was associated with a significant increase in metaphase index and reduction in micronucleus formation in dose-dependent pattern comparing with blank control and vehicle control. The results of methylthiazol tetrazolium (MTT) assay on MCF-7 tumor cells indicated a significant increase in cell proliferation inhibition in dose-dependent manner after treatment with R. chalepensis methanolic extract with potent IC50 of 51.31 µg mL-1, comparing with WRI68 normal cell line.

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