AMA 10th edition
In-text citation: (1), (2), (3), etc.
Reference: Ishmuratova MY, Tleukenova SU, Atikeyeva SN, Auelbekova АK, Zhuzbayeva GO, Zhumagaliyeva ZZ. Cryopreservation of Calendula officinalis seeds. Eurasia J Biosci. 2020;14(1), 501-505.

APA 6th edition
In-text citation: (Ishmuratova et al., 2020)
Reference: Ishmuratova, M. Y., Tleukenova, S. U., Atikeyeva, S. N., Auelbekova, А. K., Zhuzbayeva, G. O., & Zhumagaliyeva, Z. Z. (2020). Cryopreservation of Calendula officinalis seeds. Eurasian Journal of Biosciences, 14(1), 501-505.

Chicago
In-text citation: (Ishmuratova et al., 2020)
Reference: Ishmuratova, Margarita Yulaevna, Saltanat Ushkempirovna Tleukenova, Sairan Nikolayevna Atikeyeva, Аlmagul Kaliyevna Auelbekova, Gulmira Okibayevna Zhuzbayeva, and Zharkyn Zharkeyevna Zhumagaliyeva. "Cryopreservation of Calendula officinalis seeds". Eurasian Journal of Biosciences 2020 14 no. 1 (2020): 501-505.

Harvard
In-text citation: (Ishmuratova et al., 2020)
Reference: Ishmuratova, M. Y., Tleukenova, S. U., Atikeyeva, S. N., Auelbekova, А. K., Zhuzbayeva, G. O., and Zhumagaliyeva, Z. Z. (2020). Cryopreservation of Calendula officinalis seeds. Eurasian Journal of Biosciences, 14(1), pp. 501-505.

MLA
In-text citation: (Ishmuratova et al., 2020)
Reference: Ishmuratova, Margarita Yulaevna et al. "Cryopreservation of Calendula officinalis seeds". Eurasian Journal of Biosciences, vol. 14, no. 1, 2020, pp. 501-505.

Vancouver
In-text citation: (1), (2), (3), etc.
Reference: Ishmuratova MY, Tleukenova SU, Atikeyeva SN, Auelbekova АK, Zhuzbayeva GO, Zhumagaliyeva ZZ. Cryopreservation of Calendula officinalis seeds. Eurasia J Biosci. 2020;14(1):501-5.

Abstract

The article presents the results of cryopreservation of seeds of the medical plant Calendula officinalis. Cryopreservation is a highly promising direction of biology, allowing deep freezing of objects with full preservation of life properties. Calendula seeds have different size and weight. The best survival rates are observed for large seeds (germination rate - 77.5%, energy of germination - 55.0%). Assessment of seed survival in the storage rate showed a linear decrease in germination rate and energy of germination during storage. After 1.5 years of storage, germination decreased to 30.0%.As part of the research, the container was optimized for cryopreservation of Calendula officinalis seeds, defrosting conditions, seed humidity and the use of different cryoprotectors. The results showed that the optimal container for freezing in liquid nitrogen was plastic cryotubes; defrosting should be carried out at room temperature; the best survival rates were found at seed humidity 5-6%; the use of cryoprotectors was not feasible. The results of the studies can be used to organize long-term storage of Calendula officinalis seeds at extra low temperatures (liquid nitrogen).

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