The article presents the results of cryopreservation of seeds of the medical plant Calendula officinalis. Cryopreservation is a highly promising direction of biology, allowing deep freezing of objects with full preservation of life properties. Calendula seeds have different size and weight. The best survival rates are observed for large seeds (germination rate - 77.5%, energy of germination - 55.0%). Assessment of seed survival in the storage rate showed a linear decrease in germination rate and energy of germination during storage. After 1.5 years of storage, germination decreased to 30.0%.As part of the research, the container was optimized for cryopreservation of Calendula officinalis seeds, defrosting conditions, seed humidity and the use of different cryoprotectors. The results showed that the optimal container for freezing in liquid nitrogen was plastic cryotubes; defrosting should be carried out at room temperature; the best survival rates were found at seed humidity 5-6%; the use of cryoprotectors was not feasible. The results of the studies can be used to organize long-term storage of Calendula officinalis seeds at extra low temperatures (liquid nitrogen).


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