Combination of feed protein level and laserpuncture induction of broodstock catfish (Clarias sp.) to increase estrogen, vitellogenin, and egg quality
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2019 - Volume 13 Issue 2, pp. 769-779
  • Published Online: 07 Jul 2019
  • Article Views: 604 | Article Download: 373
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AMA 10th edition
In-text citation: (1), (2), (3), etc.
Reference: Hariani D, Slamet WK P. Combination of feed protein level and laserpuncture induction of broodstock catfish (Clarias sp.) to increase estrogen, vitellogenin, and egg quality. Eurasia J Biosci. 2019;13(2), 769-779.

APA 6th edition
In-text citation: (Hariani & Slamet WK, 2019)
Reference: Hariani, D., & Slamet WK, P. (2019). Combination of feed protein level and laserpuncture induction of broodstock catfish (Clarias sp.) to increase estrogen, vitellogenin, and egg quality. Eurasian Journal of Biosciences, 13(2), 769-779.

In-text citation: (Hariani and Slamet WK, 2019)
Reference: Hariani, Dyah, and Pungky Slamet WK. "Combination of feed protein level and laserpuncture induction of broodstock catfish (Clarias sp.) to increase estrogen, vitellogenin, and egg quality". Eurasian Journal of Biosciences 2019 13 no. 2 (2019): 769-779.

In-text citation: (Hariani and Slamet WK, 2019)
Reference: Hariani, D., and Slamet WK, P. (2019). Combination of feed protein level and laserpuncture induction of broodstock catfish (Clarias sp.) to increase estrogen, vitellogenin, and egg quality. Eurasian Journal of Biosciences, 13(2), pp. 769-779.

In-text citation: (Hariani and Slamet WK, 2019)
Reference: Hariani, Dyah et al. "Combination of feed protein level and laserpuncture induction of broodstock catfish (Clarias sp.) to increase estrogen, vitellogenin, and egg quality". Eurasian Journal of Biosciences, vol. 13, no. 2, 2019, pp. 769-779.

In-text citation: (1), (2), (3), etc.
Reference: Hariani D, Slamet WK P. Combination of feed protein level and laserpuncture induction of broodstock catfish (Clarias sp.) to increase estrogen, vitellogenin, and egg quality. Eurasia J Biosci. 2019;13(2):769-79.


This study determined the combined impact of various dietary protein levels (30, 35, 40%) in adult catfish feed and laserpuncture induction on enhancing the estrogen and vitellogenin levels, and egg quality, based on the fertilization rate (FR), hatching rate (HR), and survival rate (SR) after spawning. The test fish were 8–9-month-old FI hybrids obtained by cross-breeding a mature Sangkuriang female with a mature Paiton male. In total, 172 female (900–1500 g body weight) and 172 male (1140–1750 g body weight) catfish were collected from UPBAT Kepanjen District, Malang, Indonesia. Fish fed diets with increased protein levels and exposed to laserpuncture induction had significantly enhanced estrogen and vitellogenin blood serum levels (P<0.001) in weeks 3 and 6 compared to 6 weeks for the negative control. Based on the egg quality data, the addition of 40% protein in the diet of the reproductively mature females together with laserpuncture induction produced the highest FR, HR, and SR (P<0.05) compared to protein levels 30 and 35%, and the negative control.


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