Collagen fiber increase due to hydroxyapatite from crab shells (Portunus pelagicus) application in post tooth extraction in Wistar rats
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2020 - Volume 14 Issue 2, pp. 3785-3789
  • Published Online: 30 Sep 2020
  • Open Access Full Text (PDF)

Abstract

Tooth extraction will be followed by alveolar bone resorption process. Loss of alveolar bone support will affect retention, stability and comfort in the use of dentures. This can be solved by doing bone graft with hydroxyapatite (HA) material. This study used HA-derived crab shell (Portunus pelagicus) because it contains protein, calcium carbonate, and chitin. A good synthetic bone graft is the structural and composition similar to natural bone. Bone consists of collagen and HA as the main component and a few percent of the other components. The type of collagen that has an important role in bone formation is type I collagen, because this collagen can undergo the process of mineralization. Determine the enhancement of collagen fibers in the post-extraction after being given hydroxyapatite from crab shells on the 14th and 28th days. 36 Wistar rats were divided into 4 groups consisting of control and the treatment group on day 14 and day 28. HA from crab shell was applied to the rats’ sockets after extraction of lower left incisor. After the 14th and 28th days, the mandible was cut and HPA microscope slide set were made by staining Masson's Trcihome (MT). The collagen fibers was seen using a binocular light microscope at 400x magnification. The research data were analyzed using Kruskall Wallis and Mann-Whitney test. The collagen fibers increased significantly between the control and treatment groups.  Giving hydroxyapatite from crab shells in post extraction Wistar tooth sockets can increase the amount of collagen.

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