Cells catalysis is efficient methodology that has been extensively applied in various biological processes. However, industrial strains are vulnerable to environmental change, leading to poor stability and productivity. In this regards, large potentialities are embedded in immobilized cells. In particular, the immobilization techniques are of great significance in improving the catalytic performance of natural biocatalysts. Effective method of enzyme production by immobilization of microbial cells on solid career in submerged conditions has been developed. It was determined that design of proposed equipment gives the opportunity to increase enzymatic activity of immobilized cells compared to free cells by several times. A cultivation of Aspergillus oryzae M has been carried out for 49 days by immobilization of fungal cells in submerged conditions of growth. Enzymatic activity was enhanced significantly after 6 days of cultivation of immobilized cells and keeps the same value for 49 days of fungal cultivation. The alpha-amylase activity has been increased to 696 U/ml.


  • Alajwad G, Abdal AK, Al-jubori SS, Muslim SN (2020) Screening, extraction and purification for tannase produced from Iraqi Klebsiella pneumonia isolates and molecular detection of tanA gene. Eurasian Journal of Biosciences, 14(1): 259-26.
  • Bayat Z, Hassanshahian M, Cappello S (2015) Immobilization of microbes for bioremediation of crude oil polluted environments: A mini review. Open Microbiology Journal, 9: 48–54. https://doi.org/10.2174/1874285801509010048
  • Blieva R, Akhmetsadykov N, Zhakipbekova A, Kalieva A, Rakhmetova Zh (2019) Optimization of culture media for protease production by Aspergillus fungi. Asian Journal of Agriculture and Biology, 7(2):210-213.
  • Chen H, Wang M, Shen Y, Yao S (2014) Optimization of Two-species Whole-cell Immobilization System Constructed with Marine-derived Fungi and Its Biological Degradation Ability. Chinese Journal of Chemical Engineering 22(2): 187-192. https://doi.org/10.1016/S1004-9541(14)60024-0
  • Dobreva E, Ivanova V, Stefanova M, Tonkova A, Kabaivanova L, Spassova D (1998) Thermostable 1-amylase production by Bacillus licheniformis cells immobilized on polyacrylates with cyclic carbonate groups in the side chain. Microbiological Research, 153 (2): 157-162. https://doi.org/10.1016/S0944-5013(98)80035-9
  • Garde VL, Thomasset B, Barbotin J-N (1981) Electron microscopic evidence of an immobilized living cell system. Enzyme and Microbial Technology, 3(3): 216-218 https://doi.org/10.1016/0141-0229(81)90088-0
  • Hafsan, Agustina L, Natsir N, Ahmad A (2020) The stability of Phytase activity from Burkholderia sp. strain HF.7. Eurasian Journal of Biosciences, 14 (1): 991-994.
  • Kun RS, Gomes ACS, Hildén KS, Cerezo SS, Mäkelä MR, de Vries RP (2019) Developments and opportunities in fungal strain engineering for the production of novel enzymes and enzyme cocktails for plant biomass degradation, Biotechnology Advances, 37(6): 107361. https://doi.org/10.1016/j.biotechadv.2019.02.017
  • Morikawa M (2006) Beneficial biofilm formation by industrial bacteria Bacillus subtilis and related species. Journal of Bioscience & Bioengineering, 101:1–8. https://doi.org/10.1263/jbb.101.1
  • Oliveira AF, Bastos RG, de la Torre LG (2019) Bacillus subtilis immobilization in alginate microfluidic-based microparticles aiming to improve lipase productivity. Biochemical Engineering Journal, 143: 110-120. https://doi.org/10.1016/j.bej.2018.12.014
  • Patent of the Republic of Kazakhstan No. 27164. Device for the cultivation of microorganisms with filamentous structure / R.K. Blieva; applicant and patent holder RSE “Institute of Microbiology and Virology” KN MES RK - 2012 / 1110.1 applications. 24.10.2012; publ. 07/15/2013, Bul. №7. - 3p.
  • Rani G, Paresh G, Harapriya M, Vineet KG, Bhavna C (2013) Microbial a-amylases: a biotechnological perspective. Process Biochemistry, 38: 1599-1616. https://doi.org/10.1016/S0032-9592(03)00053-0
  • Sales E, Pintob M, Dorn M, Feltes BC (2020) The tale of a versatile enzyme: Alpha-amylase evolution, structure, and potential biotechnological applications for the bioremediation of n-alkanes. Chemosphere, 250: 126202. https://doi.org/10.1016/j.chemosphere.2020.126202
  • Sindhu R, Binod P, Madhavan A, Beevi US, Mathew AK, Abraham A, Pandey A, Kumar V (2017) Molecular improvements in microbial α-amylases for enhanced stability and catalytic efficiency. Bioresource Technology, 245: 1740-1748. https://doi.org/10.1016/j.biortech.2017.04.098
  • Villegas PJ (2016). Preliminary Study of Activated Carbon Filters for Pollutants Removal in Diesel Engines. International Journal of Sustainable Energy and Environmental Research, 5(2): 31-45.
  • Yu T, Wang L, Ma F, Yang J, Bai S, You J (2019) Self-immobilized biomixture with pellets of Aspergillus niger Y3 and Arthrobacter. sp ZXY-2 to remove atrazine in water: A bio-functions integration system. Science of The Total Environment, 689: 875-882. https://doi.org/10.1016/j.scitotenv.2019.06.313
  • Zhang Y, Geary T, Simpson BK (2019) Genetically modified food enzymes: a review. Current Opinion in Food Science, 25: 14-18. https://doi.org/10.1016/j.cofs.2019.01.002
  • Żur J, Piński A, Michalska J, Hupert-Kocurek K, Nowak A, Wojcieszyńska G, Guzik U (2020) A whole-cell immobilization system on bacterial cellulose for the paracetamol-degrading Pseudomonas moorei KB4 strain. International Biodeterioration & Biodegradation, 149: 104919. https://doi.org/10.1016/j.ibiod.2020.104919.


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