CAT gene polymorphism and some antioxidant levels after exercise in athletes of Babylon Gym Sport

Abstract

The multi-phenotype species has been recognized as several essential genetic different factors attributed to oxidative performance and stress in endurance events. Antioxidants like peroxidase of glutathione (GPX1), peroxide of dimethoxy (SOD) and catalase (CAT), decrease the organism’s oxidation levels. Catalase has a significant function in the reduction of hydrogen peroxide in cells of red blood, and the CAT gene determines its activity. This study concluded the role of free radicals in leading to stress of oxidative throughout practice, the current investigation was conducted in Hilla City to perform demographics, several blood variables and Catalase (CAT) gene polymorphism in athletes, the outcomes demonstrate that there are substantial variances in some demographic heterogeneity between control and athletes and all blood variables Superoxide Dismutase (SOD), Malondialdehyde, (MDA) were very high value for athletes, by using (PCR-SSCP) technique, (CAT) gene polymorphism no substantial connotation with athletes in four alleles.

References

  • Abushama HM, Abdelwahab MI, Elhassan AM, Osman OF, Mohamed HSE (2014) Antibody Response to Cutaneous Leishmaniasis in Sudan. The International Journal of Biotechnology, 3(10): 123-137.
  • Ahn J, Gammon MD, Santella RM (2005) Associations between breast cancer risk and the catalase genotype, fruit and vegetable consumption, and supplement use. Am J Epidemiol. 162: 943-952.
  • Castaldo SA, da Silva AP, Matos A, Inacio A, Bicho M, Medeiros R, Alho I, Bicho MC (2015) The role of CYBA (p22phox) and catalase genetic polymorphisms and their possible epistatic interaction in cervical cancer. Tumor biology. 36: 909-914.
  • Cooper CE, Vollaar NBJ, Choueiri T, Wilson MT (2002) Exercise, free radicals and oxidative stress. Biochem. Soc. Transactions., 30: 280-285.
  • Davey MW, Stals E, Panis B, Keulemans J, Swennen RL (2005). High-throughput determination of malondialdehyde in plant tissues. Analytical Biochemistry. 347 (2): 201-207.
  • Del Rio D, Stewart AJ, Pellegrini N (2005) A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress. Nutr Metab Cardiovasc Dis. 15(4): 316-328.
  • Dietrich M, Block G, Hudes M (2002). Antioxidant supplementation decreases lipid peroxidation biomarker F(2)-isoprostanes in plasma of smokers. Cancer Epidemiol Biomarkers Prev. 11: 7-13.
  • Farmer EE, Davoine C (2007). “Reactive electrophile species”. Curr. Opin. Plant Biol. 10 (4): 380-386.
  • Farney TM, McCarthy CG, Canale RE, Schilling BK, Whitehead PN, Bloomer RJ. (2012). Absence of blood oxidative stress in trained men after strenuous exercise. Med Sci Sports Exerc. 44(10): 1855-1863.
  • Gomez-Cabrera MC, Domenech E, Viña J (2008) Moderate exercise is an antioxidant: Upregulation of antioxidant genes by training. Free Radic Biol Med. 15;44(2): 126-131.
  • Goyal MM, Basak A (2010) Human catalase: looking for a complete identity. Protein & cell. 1: 888-897.
  • Ji L (1999) Antioxidants and oxidative stress in exercise. Proc Soc Exp Biol Med. 222: 283-292.
  • König D, Berg A (2002) Exercise and oxidative stress: Is there a need for additional antioxidants. Öster J Sports Med. 3(1): 6-15.
  • McBride JM, Kraemer WJ, Triplett-McBride T, Sebastianelli W (1997) Effect of resistance exercise on free radical production. Medicine and Science in Sports and Exercise. 30: 67-72. (cited by Viitala et al., 2004).
  • Nair V, O’Neil CL, Wang PG (2008) Malondialdehyde”, Encyclopedia of Reagents for Organic Synthesis, 2008, John Wiley & Sons, New York. https://doi.org/10.1002/047084289X.rm013
  • Radak Z, Pucsuk J, Boros S (2000) Exercise preconditioning against hydrogen peroxide-induced oxidative damage in proteins of rat myocardium. Arch Biochem Biophys. 376: 248-251.
  • Rodriguez MC, Rosenfeld J, Tarnopolsky MA (2003) Plasma malondialdehyde increases transiently after ischemic forearm exercise. Med Sci Sports Exerc. 35(11): 1859-1865.
  • Rokitzki L, Logemann E, Huber G, Keck E, Keul J (1994) αTocopherol supplementation in racing cyclists during extreme endurance training. Internat. J. Sport Nut. 4: 253-264. (cited by Viitalaet al., 2004).
  • Rousseau AS, Margaritis I, Arnaud J, Faure H, Roussel AM (2006). Physical activity alters antioxidant status in exercising elderly subjects. J Nutr Biochem 17: 463-470.
  • Tate DJ Jr, Miceli MV, Newsome DA (1995) Phagocytosis and H2O2 induce catalase and metallothionein gene expression in human retinal pigment epithelial cells. Invest Ophthalmol Vis Sci. 36: 1271-1279.
  • Viitala PE, Newhouse IJ, LaVoie N, Gottardo C (2004) The effects of antioxidant vitamin supplementation on resistance exercise induced lipid peroxidation in trained and untrained participants. Lipids Health Dis. 3: 14.
  • Young IS, Woodside JV (2001) Antioxidants in health and disease. J Clin Pathol. 54: 176-186.
  • Zhou XF, Cui J, DeStefano AL (2005) Polymorphisms in the promoter region of the catalase gene and essential hypertension. Dis Markers. 21: 3-7.

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