Carbamylated darbepoetin in combination with ethoxydol attenuates doxorubicin-induced cardiomyopathy in rats
AMA 10th edition
In-text citation: (1), (2), (3), etc.
Reference: Kolesnichenko PD, Popova IA, Sheblykin DV, et al. Carbamylated darbepoetin in combination with ethoxydol attenuates doxorubicin-induced cardiomyopathy in rats. Eurasia J Biosci. 2019;13(2), 957-963.

APA 6th edition
In-text citation: (Kolesnichenko et al., 2019)
Reference: Kolesnichenko, P. D., Popova, I. A., Sheblykin, D. V., Aldeen Azeez, A. M., Soldatov, V. O., Povetkin, S. V., . . . Stepchenko, A. A. (2019). Carbamylated darbepoetin in combination with ethoxydol attenuates doxorubicin-induced cardiomyopathy in rats. Eurasian Journal of Biosciences, 13(2), 957-963.

Chicago
In-text citation: (Kolesnichenko et al., 2019)
Reference: Kolesnichenko, Pavel D., Irina A. Popova, Dmitriy V. Sheblykin, Alhamzah Muhi Aldeen Azeez, Vladislav O. Soldatov, Sergey V. Povetkin, Galina A. Lazareva, and Alexandr A. Stepchenko. "Carbamylated darbepoetin in combination with ethoxydol attenuates doxorubicin-induced cardiomyopathy in rats". Eurasian Journal of Biosciences 2019 13 no. 2 (2019): 957-963.

Harvard
In-text citation: (Kolesnichenko et al., 2019)
Reference: Kolesnichenko, P. D., Popova, I. A., Sheblykin, D. V., Aldeen Azeez, A. M., Soldatov, V. O., Povetkin, S. V., . . . Stepchenko, A. A. (2019). Carbamylated darbepoetin in combination with ethoxydol attenuates doxorubicin-induced cardiomyopathy in rats. Eurasian Journal of Biosciences, 13(2), pp. 957-963.

MLA
In-text citation: (Kolesnichenko et al., 2019)
Reference: Kolesnichenko, Pavel D. et al. "Carbamylated darbepoetin in combination with ethoxydol attenuates doxorubicin-induced cardiomyopathy in rats". Eurasian Journal of Biosciences, vol. 13, no. 2, 2019, pp. 957-963.

Vancouver
In-text citation: (1), (2), (3), etc.
Reference: Kolesnichenko PD, Popova IA, Sheblykin DV, Aldeen Azeez AM, Soldatov VO, Povetkin SV, et al. Carbamylated darbepoetin in combination with ethoxydol attenuates doxorubicin-induced cardiomyopathy in rats. Eurasia J Biosci. 2019;13(2):957-63.

Abstract

Introduction: Doxorubicin is the drug of choice in the treatment of many malignant neoplasms, but its use is limited due to the risk of developing severe cardiomyopathy. This problem necessitates the prevention, early diagnosis and treatment of cardiomyopathy.
Material and Methods: The study was conducted on 80 male white Wistar rats, which were administered the following drugs during the experiment: doxorubicin (Teva) at a dose of 20 mg /kg, ethoxydol at a dose of 50 mg/kg, carbamylated darbepoetin at a dose of 50 mg/kg. The hearts of the rats were perfused in the installation of a Langendorf-isolated heart. All rats were recorded contractility indicators: maximum intraventricular pressure (IVP) (mm Hg), minimum IVP (mm Hg), average IVP (mm Hg), pulse IVP (mm. Hg), heart rate (HR, beats/min), maximum myocardial contraction rate (+ dP/dtmax, mm Hg/sec), maximum myocardial relaxation rate (-dP/dtmax, mm Hg/sec.), a test was performed with high-frequency stimulation. To assess the reserve capacity of the myocardium, we used the Tension-Time Index (tTTI) test of the “voltage over time” index.
Results: When used as a cardioprotector of ethoxydol at a dose of 50 mg/kg, a decrease in the toxic effect of DR and an improvement in performance by 14.8% compared with the group of DR. The degree of change in contractility indicators compared with the positive control group was 48.7%. As a result of CDEPO at a dose of 50 mg/kg, there is also a positive trend in the change in myocardial contractility, but to a lesser extent than in ethoxydol. The difference in performance compared with the DR group is 8.6%. The greatest cardioprotective effect was achieved by the introduction of a combination of DR and CDEPO. The increase in contractility compared with the doxorubicin group was 23.9%. The severity of changes in contractility indicators decreased to 38.5% compared with the positive control group. This trend in the dynamics of myocardial contractility indices can be traced both in the conditions of perfusion with norcalcium and hypercalcium solutions.
Conclusion: The most pronounced cardioprotective effect on the model of doxorubicin cardiomyopathy is determined by using a combination of carbamylated darbepoetin at a dose of 50 mkg/kg with ethoxydol at a dose of 50 mg/kg.

References

  • Bogus SK, Galenko-Yaroshevsky PA, Suzdalev KF, Sukoyan GV, Abushkevich VG (2018) 2-phenyl-1-(3-pyrrolidin-1-il-propyl)-1 H-indole hydrochloride (SS-68): Antiarrhythmic and cardioprotective activity and its molecular mechanisms of action (Part I). Research Results in Pharmacology, 4(2): 133-150. https://doi.org/10.3897/rrpharmacology.4.28592
  • Danilenko L, Klochkova G, Kizilova I, Pokrovskii M (2016) Metabolic cardioprotection: new concepts in implementation of cardioprotective effects of meldonium. Research Results in Pharmacology, 2(3): 95-100. https://doi.org/10.18413/2500-235X-2016-2-3-95-100
  • Danilenko LM (2018) Doxorubicin-associated Cardiomyopathy: New Approaches to Pharmacological Correction Using 3-(2,2,2-trimethylhydrazinium) Propionate Derivatives. Research Results in Pharmacology, 4(1): 81-86. https://doi.org/10.3897/rrpharmacology.4.25530
  • Danilenko LM, Timokhina AS, Khavanski AV, Pokrovskiy MV, Dovgan AP, Kolesnichenko, PD, et al. (2018) Method of analysis cardioprotective activity of pharmacological agents. Certificate of state registration of the PC, reg. № 2643104 from 30.01.2018. Rospatent
  • Denisuk TA, Pokrovskii MV, Philippova OV, Dolzhikov AA, Pokrovskaia TG, Korokin MV, et al. (2015) Endothelio- and cardioprotective effects of HMG-CoA reductase inhibitors under the condition of endotoxin-induced endothelial dysfunction. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 6(5): 1542-1547.
  • Denisyuk T, Lazareva G, Provotorov V, Pokrovskaya T (2016) Endothelium and cardioprotective effects of HMG-Co-A-reductase in combination with l-arginine in endothelial dysfunction modeling. Research Results in Pharmacology, 2(1): 4-8. https://doi.org/10.18413/2313-8971-2016-2-1-4-8
  • Dzhimak SS, Basov AA, Volchenko NN, Samkov AA, Fedulova LV, Baryshev MG (2017) Changes in the functional activity of mitochondria isolated from the liver of rat that passed the preadaptation to ultra-low deuterium concentration. Doklady Biochemistry and Biophysics, 476: 323–5. https://doi.org/10.1134/S1607672917050088
  • Gumanova NG, Artyushkova EB, Metel’skaya VA, Kochkarov VI, Pokrovskaya TG, Danilenko LM, et al. (2007) Effect of antioxidants pQ510 and resveratrol on regulatory function of the endothelium in rats with modeled arterial hypertension. Bulletin of Experimental Biology and Medicine, 143(6), 678-681. https://doi.org/10.1007/s10517-007-0212-x
  • Gureev VV, Alehin SA, Pokrovskiy MV, Dolghikov AA, Korokin MV, Gudyrev OS, Kolesnik IM (2014) Remote ischemic preconditioning correction in ADMA-like gestosis model. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 5(5): 1095-1098.
  • Kolesnichenko PD, Gudyrev OS, Zhuchenko MA, Zhernakova NI, Faitelson AV, Pershina MA, Soldatov VO (2018) Evaluation of carbamylated darbepoetin acute toxicity. International Journal of Research in Pharmaceutical Sciences, 9 (3): 725-730.
  • Kolesnichenko PD, Pershina MA, Gorbunova NS, Soldatov VO, Reznikov KM, Xenofontov AO (2018) The value of the redox potential of liquids in the dissolution of drugs in them. Russian Journal of Biopharmaceuticals, 10(4): 46-50.
  • Kolesnichenko PD, Reznikov KM, Zhhernakova NI, Stepchenko AA, Popova IA (2018) The Value Changes Redox System the Body Fluid Media for Life Processes and the Action of Drugs. Journal of International Pharmaceutical Research, 45: 440-444.
  • Korokina LV, Zhernakova NI, Korokin MV, Pokopejko ON (2018) Principles of pharmacological correction of pulmonary arterial hypertension. Research Results in Pharmacology, 4(2): 59-76. https://doi.org/10.3897/rrpharmacology.4.27732
  • Kukes VG, Goroshko VA (2013) Analysis of the effectiveness of the antioxidant action of the medicinal tools etilmetilgidroksipiridina malate (ethoxydole). Medicines and Rational Pharmacotherapy, 4: 26-28. (In Russian English abstract)
  • Majorova AV, Sysuev BB, Sokolova-Merkur’eva AV, Evseeva SB, Nesterova NI, Veselova VA, Puchenkova OA, Soldatov VO (2018) Gel with ectoine improves wound healing on a thermal burn model in rats. Journal of International Pharmaceutical Research, 45: 454-462.
  • Peresypkina A, Pazhinsky A, Pokrovskii M, Beskhmelnitsyna E, Pobeda A, Korokin M (2019) Correction of experimental retinal ischemia by l-isomer of ethylmethylhydroxypyridine malate. Antioxidants, 8(2). https://doi.org/10.3390/antiox8020034
  • Pokrovskii MV, Korokin MV, Kudryavtsev KV, Pokrovskaya TG, Gudyrev OS, Gureev VV, et al. (2017) Study of endothelial protective activity of phenol-derived thrombin and arginase-2 inhibitors KUD-259 and KUD-974. Bulletin of Experimental Biology and Medicine, 163(4): 436-438. https://doi.org/10.1007/s10517-017-3822-y
  • Ragulina V, Kostina D, Dovgan A, Burda Y, Nadezhdin S (2017) Nuclear factor kappa b as a potential target for pharmacological correction endothelium-associated pathology. Research Results in Pharmacology, 3(1): 114-124. https://doi.org/10.18413/2500-235X-2017-3-1-114-124
  • Rajkumar DSR, Densingh S, Gudyrev O, Faitelson A, Stepchenko A, Dolzhikov A, Povetkin S (2016) Study of the influence of L-norvaline, rosuvastatin and their combination on the level of microcirculation in bone tissue in experimental osteoporosis and fractures on its background. Research Results in Pharmacology, 2(1): 20-24. https://doi.org/10.18413/2313-8971-2016-2-1-20-24
  • Shabelnikova A (2016) Correction of ischemic damage to the retina on application of pharmacological preconditioning of recombinant erythropoietin. Research Results in Pharmacology, 2(2): 67-90. https://doi.org/10.18413/2313-8971-2016-2-2-67-90
  • Shabelnikova AS, Lutsenko VD, Pokrovskii MV, Peresipkina AA, Korokin MV, Gudyrev OS, et al. (2015) Protective effects of recombinant erythropoietin in ischemia of the retina: The role of mechanisms of preconditioning. Research Journal of Medical Sciences, 9(4): 200-203. https://doi.org/10.3923/rjmsci.2015.200.203
  • Shabelnikova AS, Peresypkina AA, Pokrovskiy MV, Kashuba AS, Netrebenko AS (2014) Analysis of the protective properties of erythropoetin and nicorandil on the basis of the model of the retina ischemia/reperfusion. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 5(6): 1335-1339.
  • Soldatov VO, Shmykova EA, Pershina MA, Ksenofontov AO, Zamitsky YM, Kulikov AL, Peresypkina AA, Dovgan AP, Belousova YV (2018) Imidazoline receptors agonists: possible mechanisms of endothelioprotection. Research Results in Pharmacology, 4(2): 11-18. https://doi.org/10.3897/rrpharmacology.4.27221
  • Trauelsen M, Rexen Ulven E, Hjorth SA, Brvar M, Monaco C, Frimurer TM, et al. (2017) Receptor structure-based discovery of non-metabolite agonists for the succinate receptor GPR91. Molecular Genetics and Metabolism, 6(12): 1585-1596. https://doi.org/10.1016/j.molmet.2017.09.005

License

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.