Buccal and labial mucosa dysplasia in Wistar rats exposed to sidestream cigarette smoke as early detection of precancerous lesions

Abstract

Background: Sidestream cigarette smoke contains cancer-causing substances. Risks of cancer, especially oral cancer, will most likely increase in people who are exposed to sidestream cigarette smoke. Dysplasia is a histopathological picture that indicates abnormal activities in the normal epithelium. The assessment of epithelial dysplasia in the oral cavity is vital to predicting malignancy development. This study aims to identify the existence of buccal and labial mucosa dysplasia in Wistar rats (Rattus norvegicus) that are exposed to sidestream cigarette smoke by observing the level of dysplasia in both buccal and labial mucosa in the fourth and eight weeks after the initial exposure.
Method: The Wistar rats were divided into three groups: group 1 that was exposed to the smoke for four weeks; group 2 that was exposed to the smoke for eight weeks; and the control group that was not exposed to any smoke. The buccal and labial mucosa of the rats were then examined histopathologically to identify the level of dysplasia, following the 2005 WHO classification. After that, the dysplasia levels were processed quantitatively and were analyzed statistically.
Results: There was a significant result of dysplasia in both group 1 and group 2 if compared to the control group.
Conclusion: The exposure of side stream cigarette smoke to Wistar rats resulted in the existence of buccal and labial mucosa dysplasia.

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