blaOXA genotyping of multidrug resistant Pseudomonas aeruginosa isolated from clinical specimens
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2020 - Volume 14 Issue 2, pp. 2941-2948
  • Published Online: 16 Sep 2020
  • Open Access Full Text (PDF)

Abstract

Background: Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen. It is a ubiquitous bacterium that is found and isolated from various environments including plants, animals, soil and humans. This bacterium accounts for 10-15% of the nosocomial infections worldwide and is considered the third most-common organism associated with hospital-acquired infections such as urinary catheter-associated infections, ventilator-associated pneumonia as well as blood, burn and wound infections, particularly involving wound infections in immunocompromised patients. Methodology: One hundred and sixty samples were collected from clinical specimens from the Babylon hospitals during the period of July-2019 to November-2019. These samples included 40 injury samples, 55 diabete-infected foot samples and 65 burn samples. Blood agar (Himedia) and MacConkey agar (Himedia) were used to isolated this bacterium, using the streaking technique and identified depending on their morphological properties (cultural and microscopical), biochemical tests, and then confirmed by PCR-sequencing for universal 16S rRNA gene. An antibiotic susceptibility tests were performed using clinical and laboratory standards institute guideline (2019). Results: The results revealed that only 30 isolates were P. aeruginosa. The highest resistant percentages toward the antibiotics were found with ceftazidime and cefotaxime (96.7 %), while the lowest resistant percentages were found with colistin and polymyxin B (40 %). The ability of these isolates to produce OXA β-lactamase was investigated using PCR, it was found that 26.6% of the isolates belong to OXA-I, 40% of the isolates belong to OXA-III and no one of these isolates belong to OXA-II. Conclusion: The rates of OXA-type β-lactamases producing P. aeruginosa isolates from the clinical specimens were notable, therefore, the management and the treatment strategies should be revised and the proper use of the infection-control measures is needed to reduce the spread of the resistant genes in the isolates of P. aeruginosa.

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