Silver has a good history of use in medicine and people healthcare. The biosynthesis of silver nanoparticles (AgNPs) was prepared by the ethanolic extract of Hibiscus sabdariffa. The UV, FTIR, and SEM were used for the detection of AgNPs. We have compared the antibacterial activity between the ethanolic extract and nano extract. The antibacterial activity was high in nano extract. DPPH assay was estimated the antioxidant action of nano extract and the effects was showed 82.22 % in 75μg/ml concentration. MTT assay was used for detecting the cytotoxicity of nano extract against Colon cancer, the outcomes showed the moral level of cytotoxic action with increasing concentration.

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