In this article the influence of co-cultivation of the micromycetes strains Tr. viride 121 and A. awamori F-RKM 0719 and on the biosynthesis of the cellulose complex enzymes released by them into the medium with a deep cultivation method. The choice of these microorganisms for the creation of micromycetes consortium is due to their biochemical and morphological characteristics: Tr. viride 121 is a highly active producer of cellulase, xylanases; A. awamori F-RKM 0719 is a highly active producer of a pectolytic enzymes complex as well as celluloses and β-gluconases. The dynamics of enzymes biosynthesis and the growth rate under conditions of co-cultivation is studied. The obtained data testify to the existence of an inverse relationship between the rate of cellulase synthesis and the growth rate of the studied micromycetes consortium cells. Activation of the enzyme synthesis complex was noted when a suspension of methylcellulose was injected into the medium and A.awamori strain F-RKM 0719 was seeded at the end of the exponential growth phase of strain Tr. viride 121. The advantage of mycelial mixed cultures deep cultivation microorganisms experimentally established, which provides an increase in the productivity of strains in 1.5-3.2 times due to more effective utilization of substrates and synergism of hydrolytic enzymes. In addition, the pH and thermostability of cellulolytic enzymes studied in the joint cultivation of microscopic fungi. The results of experiments indicate the stability of cellulases in the pH range of 4.0-5.0 at a temperature of 30-60°C which allows increasing the efficiency of processing plant material biotechnological processes. Preparation of a complete cellulase complex by co-culturing Tr. viride 121 and A. awamori F-RKM 0719 is more economically attractive for deep processing of plant raw materials.


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