Biosynthesis of cellulase with Trichoderma viride and Aspergillus awamori micromycetes in co-cultivation
Zhaksylyk Makhatov 1 * , Bakhytzhan Kedelbayev 1, Peter Lieberzeit 2, Madina Dzhakashyeva 1, Zhanar Elemanova 1, Roza Abildayeva 1, Rustem Altynbekov 1, Usen Akhanov 1, Bibigul Doltayeva 3, Azhar Omirbayeva 3, Zakhro Akhmedova 4
1 M.Auezov South Kazakhstan State University, Shymkent, KAZAKHSTAN2 University of Vienna, Vienna, AUSTRIA3 South-Kazakhstan Medical Academy, Shymkent, KAZAKHSTAN4 Institute of Microbiology of Academy of Sciences of the Republic of Uzbekistan, Tashkent, UZBEKISTAN
* Corresponding Author
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2019 - Volume 13 Issue 2, pp. 1521-1526
  • Published Online: 14 Oct 2019
  • Article Views: 465 | Article Download: 283
  • Open Access Full Text (PDF)
AMA 10th edition
In-text citation: (1), (2), (3), etc.
Reference: Makhatov Z, Kedelbayev B, Lieberzeit P, et al. Biosynthesis of cellulase with Trichoderma viride and Aspergillus awamori micromycetes in co-cultivation. Eurasia J Biosci. 2019;13(2), 1521-1526.

APA 6th edition
In-text citation: (Makhatov et al., 2019)
Reference: Makhatov, Z., Kedelbayev, B., Lieberzeit, P., Dzhakashyeva, M., Elemanova, Z., Abildayeva, R., . . . Akhmedova, Z. (2019). Biosynthesis of cellulase with Trichoderma viride and Aspergillus awamori micromycetes in co-cultivation. Eurasian Journal of Biosciences, 13(2), 1521-1526.

Chicago
In-text citation: (Makhatov et al., 2019)
Reference: Makhatov, Zhaksylyk, Bakhytzhan Kedelbayev, Peter Lieberzeit, Madina Dzhakashyeva, Zhanar Elemanova, Roza Abildayeva, Rustem Altynbekov, Usen Akhanov, Bibigul Doltayeva, Azhar Omirbayeva, and Zakhro Akhmedova. "Biosynthesis of cellulase with Trichoderma viride and Aspergillus awamori micromycetes in co-cultivation". Eurasian Journal of Biosciences 2019 13 no. 2 (2019): 1521-1526.

Harvard
In-text citation: (Makhatov et al., 2019)
Reference: Makhatov, Z., Kedelbayev, B., Lieberzeit, P., Dzhakashyeva, M., Elemanova, Z., Abildayeva, R., . . . Akhmedova, Z. (2019). Biosynthesis of cellulase with Trichoderma viride and Aspergillus awamori micromycetes in co-cultivation. Eurasian Journal of Biosciences, 13(2), pp. 1521-1526.

MLA
In-text citation: (Makhatov et al., 2019)
Reference: Makhatov, Zhaksylyk et al. "Biosynthesis of cellulase with Trichoderma viride and Aspergillus awamori micromycetes in co-cultivation". Eurasian Journal of Biosciences, vol. 13, no. 2, 2019, pp. 1521-1526.

Vancouver
In-text citation: (1), (2), (3), etc.
Reference: Makhatov Z, Kedelbayev B, Lieberzeit P, Dzhakashyeva M, Elemanova Z, Abildayeva R, et al. Biosynthesis of cellulase with Trichoderma viride and Aspergillus awamori micromycetes in co-cultivation. Eurasia J Biosci. 2019;13(2):1521-6.

Abstract

In this article the influence of co-cultivation of the micromycetes strains Tr. viride 121 and A. awamori F-RKM 0719 and on the biosynthesis of the cellulose complex enzymes released by them into the medium with a deep cultivation method. The choice of these microorganisms for the creation of micromycetes consortium is due to their biochemical and morphological characteristics: Tr. viride 121 is a highly active producer of cellulase, xylanases; A. awamori F-RKM 0719 is a highly active producer of a pectolytic enzymes complex as well as celluloses and β-gluconases. The dynamics of enzymes biosynthesis and the growth rate under conditions of co-cultivation is studied. The obtained data testify to the existence of an inverse relationship between the rate of cellulase synthesis and the growth rate of the studied micromycetes consortium cells. Activation of the enzyme synthesis complex was noted when a suspension of methylcellulose was injected into the medium and A.awamori strain F-RKM 0719 was seeded at the end of the exponential growth phase of strain Tr. viride 121. The advantage of mycelial mixed cultures deep cultivation microorganisms experimentally established, which provides an increase in the productivity of strains in 1.5-3.2 times due to more effective utilization of substrates and synergism of hydrolytic enzymes. In addition, the pH and thermostability of cellulolytic enzymes studied in the joint cultivation of microscopic fungi. The results of experiments indicate the stability of cellulases in the pH range of 4.0-5.0 at a temperature of 30-60°C which allows increasing the efficiency of processing plant material biotechnological processes. Preparation of a complete cellulase complex by co-culturing Tr. viride 121 and A. awamori F-RKM 0719 is more economically attractive for deep processing of plant raw materials.

Keywords

biosynthesis mycelial fungus cellulolytic enzymes glucose polysaccharides cultivation consortium

References

  • Abbasi T, Abbasi SA (2010) Biomass energy and the environmental impacts associated with its production and utilization. Renewable and Sustainable Energy Reviews. Elsevier, 14(3): 919-937. https://doi.org/10.1016/j.rser.2009.11.006
  • Al-Kharousi M.M., Sivakumar N., Elshafie A. (2015) Characterization of cellulase enzyme produced by chaetomium sp. Isolated from books and archives. EurAsian Journal of BioSciences, 9: 52-60. https://doi.org/10.5053/ejobios.2015.9.0.7
  • Bazunova MV, Fakhretdinov RK, Galiev LR, et al. (2018) Influence of biodestruction on deformation-strength properties of polymer composites based on secondary polypropylene and natural components of plant origin. Perspektivnye Materialy, 5: 50-59. https://doi.org/10.30791/1028-978x-2018-5-50-59
  • Dzhakasheva MA, Kedelbaev BSh, Liberzeit P (2017) Novel Enzyme Preparation For Winemaking. Proceedings Of Universities Applied Chemistry And Biotechnology, 7(3): 46-53. https://doi.org/10.21285/2227-2925-2017-7-3-46-53
  • GOST (2014) №55298-2012. Enzyme preparations for the food industry. Methods for determining pectolytic activity. Introd. 2014-01-01. Moscow: Standartinform.24.
  • Grunwald P (2014) Industrial Biocatalysis. Pan Stanford Publishing Pte. Ltd., USA: 205-232. https://doi.org/10.1201/b17828
  • Hamelinck CN, Hooijdonk GV, Faaij APC (2005) Prospects for Ethanol from Lignocellulosic Biomass: techno-economic performance in short-, middle- and long-term. Biomass and Bioenergy, 28(4): 384-410. https://doi.org/10.1016/j.biombioe.2004.09.002
  • Heinze T, Seoud OA, El Koschella A (2018) Structure and Properties of Cellulose and Its Derivatives. Cellulose Derivatives: 39-172. https://doi.org/10.1007/978-3-319-73168-1_2
  • Hoelzle RD, Virdis B, Batstone DJ (2014) Regulation mechanisms in mixed and pure culture microbial fermentation. Biotechnology and Bioengineering, 111(11): 2139-2154. https://doi.org/10.1002/bit.25321
  • Ivanova C, Baath JA, Seiboth B, Kubicek CP (2013) Systems Analysis of Lactose Metabolism in Trichoderma reesei Identifies a Lactose Permease That Is Essential for Cellulase Induction. PLoS ONE, 8(5): e62631. https://doi.org/10.1371/journal.pone.0062631
  • Karmakar M, Ray RR (2011) Current trends in research and application of microbial cellulases. Research J. Microbiology, 6(1): 41-53. https://doi.org/10.3923/jm.2011.41.53
  • Kisten AG, Kurdish IK, Bega ZT, Tsarenko IYu (2006) The effects of several factors on the growth of pure and mixed cultures of Azotobacter chroococcum and Bacillus subtilis. Applied Biochemistry and Microbiology, 42(3): 278-283. https://doi.org/10.1134/s0003683806030100
  • König J, Grasser R, Pikor H, Vogel K (2002) Determination of xylanase, β-glucanase, and cellulase activity. Analytical and Bioanalytical Chemistry, 374(1): 80-87. https://doi.org/10.1007/s00216-002-1379-7
  • Kumar A, Aggarwal D, Yadav M, Kumar P, Kumar V (2019) 3. Biotechnological conversion of plant biomass into value-added products. Industrial Biotechnology, 51-72. https://doi.org/10.1515/9783110563337-003
  • Nsengiyumva DS, Balabanov PA, Kiseleva IS (2019) Impact of fungal biologically active substances on plant growth.Proceedings Article published 2019. https://doi.org/10.1063/1.5087372
  • Prasanna BD, Gummadi SN, Vadlani PV. (2016) Biotechnology and Biochemical Engineering. Book published 2016. https://doi.org/10.1007/978-981-10-1920-3
  • Rimareva LV, Serba ЕМ, Overchenko МB, et al. (1970) Multipurpose Utilization of The Aspergillus Oryzae Fungus - The Producer Of The Hydrolase Complex. Vestnik of the Russian agricultural science, 5: 29-33. https://doi.org/10.30850/vrsn/2018/5/29-33
  • Sandhya A, Sridevi A, Suvarnalathadevi P (2019) Biochemical characterization of phytase purified from aspergillus niger S2. EurAsian Journal of BioSciences, 13(1): 99-103.
  • Saparbekova AA, Mutaliyeva BZh, Dzhakasheva MA, Latif AS (2018) Extraction of resveratrol with the enzyme preparation Pectinol F-RKM 0719 produced by the strain Aspergillus awamori F-RKM 0719. Experimental Biology, 77(4): 96-106. https://doi.org/10.26577/eb-2018-4-1367
  • Sharma P, Kumar V, Naik B, Bisht GS (2013) Screening of xylanase activity of Streptomyces albidoflavus PSM-3n isolated from Uttarakhand. EurAsian Journal of BioSciences, 1: 30-40. https://doi.org/10.5053/ejobios.2013.7.0.4
  • Van Nostrand’s Encyclopedia of Chemistry (2015) Enzyme Preparations. https://doi.org/10.1002/0471740039.vec0931
  • Vidogradova SP, Kushnir SN (2003) Biosynthesis of hydrolytic enzymes in the joint cultivation of macro- and micromycetes. Applied Biochemistry & Microbiology, 39(6): 573-575. https://doi.org/10.1023/a:1026226417542
  • Volf I, Popa VI (2018) Biomass as Renewable Raw Material to Obtain Bioproducts of High-Tech Value. Elsevier: 19-54. https://doi.org/10.1016/b978-0-444-63774-1.05001-8
  • Wang Y, Song X, Zhang S, Shu Z, He C, Huang Q, Yao L (2016) Improving the activity of Trichoderma reesei cel7B through stabilizing the transition state. Biotechnol Bioeng, 113(6): 1171-1177. https://doi.org/10.1002/bit.25887

License

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
EurAsian Journal of BioSciences © 2007-2020 | EurAsia J BioSci