Biosynthesis of cellulase with Trichoderma viride and Aspergillus awamori micromycetes in co-cultivation
Zhaksylyk Makhatov 1 * , Bakhytzhan Kedelbayev 1, Peter Lieberzeit 2, Madina Dzhakashyeva 1, Zhanar Elemanova 1, Roza Abildayeva 1, Rustem Altynbekov 1, Usen Akhanov 1, Bibigul Doltayeva 3, Azhar Omirbayeva 3, Zakhro Akhmedova 4
1 M.Auezov South Kazakhstan State University, Shymkent, KAZAKHSTAN2 University of Vienna, Vienna, AUSTRIA3 South-Kazakhstan Medical Academy, Shymkent, KAZAKHSTAN4 Institute of Microbiology of Academy of Sciences of the Republic of Uzbekistan, Tashkent, UZBEKISTAN
* Corresponding Author
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2019 - Volume 13 Issue 2, pp. 1521-1526
  • Published Online: 14 Oct 2019
  • Article Views: 229 | Article Download: 140
  • Open Access Full Text (PDF)
AMA 10th edition
In-text citation: (1), (2), (3), etc.
Reference: Makhatov Z, Kedelbayev B, Lieberzeit P, et al. Biosynthesis of cellulase with Trichoderma viride and Aspergillus awamori micromycetes in co-cultivation. Eurasia J Biosci. 2019;13(2), 1521-1526.

APA 6th edition
In-text citation: (Makhatov et al., 2019)
Reference: Makhatov, Z., Kedelbayev, B., Lieberzeit, P., Dzhakashyeva, M., Elemanova, Z., Abildayeva, R., . . . Akhmedova, Z. (2019). Biosynthesis of cellulase with Trichoderma viride and Aspergillus awamori micromycetes in co-cultivation. Eurasian Journal of Biosciences, 13(2), 1521-1526.

Chicago
In-text citation: (Makhatov et al., 2019)
Reference: Makhatov, Zhaksylyk, Bakhytzhan Kedelbayev, Peter Lieberzeit, Madina Dzhakashyeva, Zhanar Elemanova, Roza Abildayeva, Rustem Altynbekov, Usen Akhanov, Bibigul Doltayeva, Azhar Omirbayeva, and Zakhro Akhmedova. "Biosynthesis of cellulase with Trichoderma viride and Aspergillus awamori micromycetes in co-cultivation". Eurasian Journal of Biosciences 2019 13 no. 2 (2019): 1521-1526.

Harvard
In-text citation: (Makhatov et al., 2019)
Reference: Makhatov, Z., Kedelbayev, B., Lieberzeit, P., Dzhakashyeva, M., Elemanova, Z., Abildayeva, R., . . . Akhmedova, Z. (2019). Biosynthesis of cellulase with Trichoderma viride and Aspergillus awamori micromycetes in co-cultivation. Eurasian Journal of Biosciences, 13(2), pp. 1521-1526.

MLA
In-text citation: (Makhatov et al., 2019)
Reference: Makhatov, Zhaksylyk et al. "Biosynthesis of cellulase with Trichoderma viride and Aspergillus awamori micromycetes in co-cultivation". Eurasian Journal of Biosciences, vol. 13, no. 2, 2019, pp. 1521-1526.

Vancouver
In-text citation: (1), (2), (3), etc.
Reference: Makhatov Z, Kedelbayev B, Lieberzeit P, Dzhakashyeva M, Elemanova Z, Abildayeva R, et al. Biosynthesis of cellulase with Trichoderma viride and Aspergillus awamori micromycetes in co-cultivation. Eurasia J Biosci. 2019;13(2):1521-6.

Abstract

In this article the influence of co-cultivation of the micromycetes strains Tr. viride 121 and A. awamori F-RKM 0719 and on the biosynthesis of the cellulose complex enzymes released by them into the medium with a deep cultivation method. The choice of these microorganisms for the creation of micromycetes consortium is due to their biochemical and morphological characteristics: Tr. viride 121 is a highly active producer of cellulase, xylanases; A. awamori F-RKM 0719 is a highly active producer of a pectolytic enzymes complex as well as celluloses and β-gluconases. The dynamics of enzymes biosynthesis and the growth rate under conditions of co-cultivation is studied. The obtained data testify to the existence of an inverse relationship between the rate of cellulase synthesis and the growth rate of the studied micromycetes consortium cells. Activation of the enzyme synthesis complex was noted when a suspension of methylcellulose was injected into the medium and A.awamori strain F-RKM 0719 was seeded at the end of the exponential growth phase of strain Tr. viride 121. The advantage of mycelial mixed cultures deep cultivation microorganisms experimentally established, which provides an increase in the productivity of strains in 1.5-3.2 times due to more effective utilization of substrates and synergism of hydrolytic enzymes. In addition, the pH and thermostability of cellulolytic enzymes studied in the joint cultivation of microscopic fungi. The results of experiments indicate the stability of cellulases in the pH range of 4.0-5.0 at a temperature of 30-60°C which allows increasing the efficiency of processing plant material biotechnological processes. Preparation of a complete cellulase complex by co-culturing Tr. viride 121 and A. awamori F-RKM 0719 is more economically attractive for deep processing of plant raw materials.

Keywords

biosynthesis mycelial fungus cellulolytic enzymes glucose polysaccharides cultivation consortium

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