Biological study of changes in transaminases and lactate dehydrogenase in the blood of heifers of different cattle breeds
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2019 - Volume 13 Issue 1, pp. 523-527
  • Published Online: 12 May 2019
  • Article Views: 31 | Article Download: 34
  • Open Access Full Text (PDF)
AMA 10th edition
In-text citation: (1), (2), (3), etc.
Reference: Rotmistrovskaya EG, Eremenko VI. Biological study of changes in transaminases and lactate dehydrogenase in the blood of heifers of different cattle breeds. Eurasia J Biosci. 2019;13(1), 523-527.

APA 6th edition
In-text citation: (Rotmistrovskaya & Eremenko, 2019)
Reference: Rotmistrovskaya, E. G., & Eremenko, V. I. (2019). Biological study of changes in transaminases and lactate dehydrogenase in the blood of heifers of different cattle breeds. Eurasian Journal of Biosciences, 13(1), 523-527.

Chicago
In-text citation: (Rotmistrovskaya and Eremenko, 2019)
Reference: Rotmistrovskaya, E. G., and V. I. Eremenko. "Biological study of changes in transaminases and lactate dehydrogenase in the blood of heifers of different cattle breeds". Eurasian Journal of Biosciences 2019 13 no. 1 (2019): 523-527.

Harvard
In-text citation: (Rotmistrovskaya and Eremenko, 2019)
Reference: Rotmistrovskaya, E. G., and Eremenko, V. I. (2019). Biological study of changes in transaminases and lactate dehydrogenase in the blood of heifers of different cattle breeds. Eurasian Journal of Biosciences, 13(1), pp. 523-527.

MLA
In-text citation: (Rotmistrovskaya and Eremenko, 2019)
Reference: Rotmistrovskaya, E. G. et al. "Biological study of changes in transaminases and lactate dehydrogenase in the blood of heifers of different cattle breeds". Eurasian Journal of Biosciences, vol. 13, no. 1, 2019, pp. 523-527.

Vancouver
In-text citation: (1), (2), (3), etc.
Reference: Rotmistrovskaya EG, Eremenko VI. Biological study of changes in transaminases and lactate dehydrogenase in the blood of heifers of different cattle breeds. Eurasia J Biosci. 2019;13(1):523-7.

Abstract

Biologically the most important factor that influences development of dairy and beef cattle breeding is the selection of cattle. For this purpose, a detailed biological study of interior and exterior indicators, which would reflect the breed features of cattle, is required. It is known that blood metabolites are closely correlated with the level of milk production. In addition, metabolic reactions are extremely consistent with each other. A special role is played by transaminases: alanine aminotransferase, aspartate aminotransferase - blood plasma enzymes and lactate dehydrogenase, which catalyzes the oxidation of lactic acid to pyruvic acid. In this regard, it is necessary to study the activity of these enzymes in cattle of different breeds. Objective and tasks of the research. Objective of this paper was to study the activity of AST, ALT and LDH in black-and-white Holstein, Simmental, Aberdeen-Angus heifers, as well as in crossbred heifers (Simmental’s Aberdeen-Angus). The experiments involved all breeds, 10 animals from each, of similar age. The level of feeding of animals was the same and corresponded to the level of their physiological state. Blood for the study of enzymes was collected from the tail vein, before the morning feeding once in a month in the neonatal period, at the age of 3, 6, 12 and 15 months. The activity of the AST, ALT and LDH enzymes was determined on a Saphire 400 biochemical analyzer using standard BioSistems reagent kits. The experimental data were processed using Student’s t-test in Microsoft Office Excel 2007. It follows from the analysis that the activity of ALT, AST and LDH increases with increasing age of the heifers from birth to 15 months of age. In the black-and-white Holstein and Simmental heifers the activity of ALT and AST is lower compared to the compared group of Aberdeen-Angus breed and crossbred animals. Opposite data were obtained in the study of the enzyme LDH; thus, the activity of LDH in the blood of black-and-white Holstein and Simmental heifers is slightly higher compared to the group of Aberdeen-Angus breed and crossbred animals.

References

  • Budinkova AV, Pasechnik AP, Fotiadis FV (1985) Relationship of biochemical parameters of blood of heifers and their growth energy and body weight. Bulletin of All-Russian Institute of Livestock Breeding. Podolsk, (78): 29-31.
  • Dikson M, Webb E (1997) Enzymes. (Trans. LM Ginodman and MI Leviant, ed. AI Oparin) M.: Mir, 1966. 816 p.
  • Ersoy AÖ, Öztaş E, Özler S, Ersoy E, Topçu HO, Fındık RB, et al. (2017) The evaluation of the low risk pregnant women who gave birth to macrosomic infants. J Clin Exp Invest., 8(4): 114-9. https://doi.org/10.5799/jcei.382414
  • Htwe O, Naicker AS, Pei TS (2016) Spinal epidural hematoma due to anticoagulant therapy: a case report and literature review. European Journal of General Medicine, 13(3): 61-2. https://doi.org/10.29333/ejgm/81908
  • Kyselovic J, Debreceni O (1990) Zmeny biochemickych ukazovatel’ov v krynom sere teliat po stresovej zatazi. Acta zootechn. Nitra, (46): 53-9.
  • Lopez Alonso M, Miranda M, Hernandez J, Castillo C, Ayala I, Benedito JL (1997) Seasonal variation of glutathione peroxidase, CK and AST in sheep in a low-selenium region. J. Anim. Feed Sc., 6(3): 343-51. https://doi.org/10.22358/jafs/69530/1997
  • Rabbani M, Bagherzadeh N, Rafiei H (2014) Calculating raw material and work-in-process inventories in MTO. MTS production. UCT Journal of Research in Science, Engineering and Technology, 2(3): 109-16.
  • Radchenkov VP, Matveev VA (1997) Methods of blood test. Methods of bio-chemical analysis (reference book). Borovsk: 165-200.
  • Stoianovskii VG, Aksenova GV, Spitinskii VV (1989) Influence of chlorpromazine and phenazepam on the development of adaptive reactions and productivity in young cattle under transport-induced stress. Abstracts of reports. New pharmacological agents in veterinary medicine: 98-9.
  • Trifonova LR (1976) Activity of cattle blood enzymes associated with sex, age, milk production, and season of the year. Works of North-Western Institute of Dairy and Grassland Farming. Arkhangelsk, 9: 166-71.
  • Vladimirov VL, Milushev RK (1988) Biochemical processes in purebred and crossbred heifers in ontogenesis. Bulletin of scientific papers. All-Russian Institute of Livestock Breeding. Podolsk, 92: 77-80.
  • Vladimirov VL, Milushev RK (1992) Biochemical processes in purebred and crossbred heifers in ontogenesis. Bulletin of scientific papers. All-Russian Institute of Livestock Breeding. Podolsk, 108: 96-9.

License

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.