Biological importance of milk oligosaccharides isolated from Gaddi sheep’s milk
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2019 - Volume 13 Issue 2, pp. 1245-1249
  • Published Online: 18 Sep 2019
  • Article Views: 62 | Article Download: 26
  • Open Access Full Text (PDF)
AMA 10th edition
In-text citation: (1), (2), (3), etc.
Reference: Shahi S, Singh SK. Biological importance of milk oligosaccharides isolated from Gaddi sheep’s milk. Eurasia J Biosci. 2019;13(2), 1245-1249.

APA 6th edition
In-text citation: (Shahi & Singh, 2019)
Reference: Shahi, S., & Singh, S. K. (2019). Biological importance of milk oligosaccharides isolated from Gaddi sheep’s milk. Eurasian Journal of Biosciences, 13(2), 1245-1249.

Chicago
In-text citation: (Shahi and Singh, 2019)
Reference: Shahi, Sanyogita, and Shirish Kumar Singh. "Biological importance of milk oligosaccharides isolated from Gaddi sheep’s milk". Eurasian Journal of Biosciences 2019 13 no. 2 (2019): 1245-1249.

Harvard
In-text citation: (Shahi and Singh, 2019)
Reference: Shahi, S., and Singh, S. K. (2019). Biological importance of milk oligosaccharides isolated from Gaddi sheep’s milk. Eurasian Journal of Biosciences, 13(2), pp. 1245-1249.

MLA
In-text citation: (Shahi and Singh, 2019)
Reference: Shahi, Sanyogita et al. "Biological importance of milk oligosaccharides isolated from Gaddi sheep’s milk". Eurasian Journal of Biosciences, vol. 13, no. 2, 2019, pp. 1245-1249.

Vancouver
In-text citation: (1), (2), (3), etc.
Reference: Shahi S, Singh SK. Biological importance of milk oligosaccharides isolated from Gaddi sheep’s milk. Eurasia J Biosci. 2019;13(2):1245-9.

Abstract

Carbohydrate is a are the firewood of life Complex carbohydrates of glycoproteins, glycolipids and bacterial polysaccharides encode information relevant to cell interaction and that the information is decoded by binding to lectins and other proteins. Bioactive carbohydrates are crucial in mediating essential biological processes, and their biosynthesis is an essential aspect to develop for a global view of their biological functions. Recent scientific studies have demonstrated that sugars also play fundamental roles in the regulation of biological activity and consequently, in the cause and treatment of many diseases as well as in drug action. Oligosaccharides are essential constituents of all living beings i.e. bacteria, fungi, plants and placental mammals’ milk. The milk is a rich source of oligosaccharides and their composition depends on their origin in which mammals the milk belongs. Buffalo milk oligosaccharides have resistivity against parasitic infections. Donkey milk oligosaccharides have ability to stimulate non-specific and specific immunological resistance. Goat milk oligosaccharides is useful for intestinal protection and repair after a damage caused by DSS (Dextron sodium sulphate)-induced colitis and their implication in human intestinal inflammation. Goat milk oligosaccharides have anti-inflammatory effects. The cows’ milk oligosaccharides reduce the adhesion of enterotoxic Escherichia coli strains of the calf. Keeping in mind all bioactive properties of different origin, Gaddi sheep’s milk was collected and processed by Modified method of Kobata and Ginsburg and then it was purified by Sephadex G-25 Gel column. The homogeneity is confirmed by reverse phase high performance chromatography and supported by thin layer chromatography. The acetylation of oligosaccharides mixture followed by the silica gel chromatography led to isolation of a novel oligosaccharides. The biological activity of Gaddi sheep’s milk oligosaccharides was investigated by MTT assay, it is a type of colorimetric assay that measures the reduction of yellow 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) by mitochondrial succinate dehydrogenase. The MTT enters the cells and passes into the mitochondria where it is reduced to an insoluble, coloured (dark purple) formazan product. The cells are then solubilised with an organic solvent (e.g. isopropanol) and the released, solubilised formazan reagent is measured spectrophotometrically. Since reduction of MTT can only occur in metabolically active cells the level of activity is a measure of the viability of the cells.

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